The mechanisms of antibiotic action can be highly context specific. Using in-cell cryo-electron tomography (cryo-ET) of Mycoplasma pneumoniae — a causative agent of respiratory disease — we visualized the context-dependent action of the ribosome-targeting antibiotic chloramphenicol simultaneously at atomic, molecular and cellular levels.

抗生素作用的机制可能具有高度的背景特异性。使用肺炎支原体（呼吸道疾病的病原体）的细胞内冷冻电子断层扫描（cryo-ET），我们在原子，分子和细胞水平上同时观察了核糖体靶向抗生素氯霉素的背景依赖性作用。

This work highlights how in-cell structural biology can expand our understanding of antibiotic mechanisms of action..

这项工作强调了细胞结构生物学如何扩大我们对抗生素作用机制的理解。。

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Fig. 1: Chloramphenicol-bound ribosomes from inside M. pneumoniae cells.

图1：来自肺炎支原体细胞内部的氯霉素结合的核糖体。

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。自然方法18186-193（2021）。本文报道了从细胞冷冻ET数据实现核糖体高分辨率重建的算法突破。文章。

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Download referencesAdditional informationPublisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.This is a summary of: Xue, L., Spahn, C. M. T., Schacherl, M. & Mahamid, J. Structural insights into context-dependent inhibitory mechanisms of chloramphenicol in cells.

下载参考其他信息出版商的注释Springer Nature对于已发布地图和机构隶属关系中的管辖权主张保持中立。这是以下内容的总结：Xue，L.，Spahn，C.M.T.，Schacherl，M。＆Mahamid，J。对氯霉素在细胞中的上下文依赖性抑制机制的结构见解。

Nat. Struct. Mol. Biol. https://doi.org/10.1038/s41594-024-01441-0 (2024).Rights and permissionsReprints and permissionsAbout this articleCite this article Visualizing antibiotic action in a pathogenic bacterium at atomic to cellular scale..

自然结构。分子生物学。https://doi.org/10.1038/s41594-024-01441-0（2024年）。权利和许可打印和许可本文引用本文，在原子到细胞的范围内可视化病原菌中的抗生素作用。。

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