NEW HAVEN, Conn. and RESEARCH TRIANGLE PARK, N.C., Jan. 23, 2024 /PRNewswire/ -- AtlasXomics and EpiCypher announce their partnership to develop CUT&Tag kits and assay services for spatial epigenomics applications. These assays will be developed on AtlasXomics' DBiT-seq platform using CUT&Tag reagents and antibodies from EpiCypher, expanding the spatial 'omics toolbox to histone post translational modifications (PTMs), transcription factors, and other chromatin regulators..

康涅狄格州纽黑文和新泽西州三角研究公园。C、 ，2024年1月23日/PRNewswire/--AtlasXomics和EpiCypher宣布合作开发用于空间表观基因组学应用的切割和标记试剂盒和分析服务。这些分析将在AtlasXomics的DBiT-seq平台上使用来自EpiCypher的CUT＆Tag试剂和抗体进行开发，将空间“组学工具箱”扩展到组蛋白翻译后修饰（PTM），转录因子和其他染色质调节剂。。

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AtlasXomics and EpiCypher announce partnership to develop CUT&Tag kits and services for spatial epigenomics applications

AtlasXomics和EpiCypher宣布合作开发用于空间表观基因组学应用的切割和标记工具包和服务

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AtlasXomics' spatial CUT&Tag profiling of RNA Pol II binding patterns in mouse brain

AtlasXomics对小鼠大脑中RNA Pol II结合模式的空间切割和标签分析

Spatial technologies enable joint analyses of cellular and molecular heterogeneity from intact tissues. The application of ultra-sensitive chromatin mapping assays, such as CUT&Tag, to spatial techniques is critical to the study of biological mechanisms in development and disease. CUT&Tag (Cleavage Under Targets and Tagmentation) was developed at Fred Hutchinson Cancer Center by Dr.

空间技术可以对完整组织的细胞和分子异质性进行联合分析。超灵敏染色质作图分析（例如CUT＆Tag）在空间技术中的应用对于研究发育和疾病的生物学机制至关重要。切割和标签（靶标和标签下的切割）是由弗雷德·哈钦森癌症中心（FredHutchinsonCancerCenter）博士开发的。

Steven Henikoff (a molecular biologist and Howard Hughes Medical Institute Investigator) and commercialized by EpiCypher. This approach uses a fusion of Tn5 transposase, protein A, and protein G (pAG-Tn5) to cleave and add sequencing adapters at antibody-bound chromatin. Due to its remarkable sensitivity and adaptability, CUT&Tag has emerged as the leading platform for single-cell and spatial epigenomics applications..

史蒂文·海尼科夫（分子生物学家和霍华德·休斯医学研究所研究员），并被EpiCypher商业化。该方法使用Tn5转座酶，蛋白a和蛋白G（pAG-Tn5）的融合体在抗体结合的染色质上切割和添加测序衔接子。由于其显着的灵敏度和适应性，CUT＆Tag已成为单细胞和空间表观基因组学应用的领先平台。。

AtlasXomics' DBiT-seq (Deterministic Barcoding in Tissue for Spatial Omics Sequencing) platform was originally developed by Professor Rong Fan, Harold Hodgkinson Professor of Biomedical Engineering at Yale University. DBiT-seq utilizes a combination of microfluidics and next-generation sequencing for high-resolution spatial profiling in tissues.

AtlasXomics的DBiT-seq（用于空间组学测序的组织中的确定性条形码）平台最初由耶鲁大学生物医学工程哈罗德·霍奇金森教授荣凡教授开发。DBiT-seq利用微流体和下一代测序的组合来进行组织中的高分辨率空间分析。

DBiT-seq is rapidly gaining traction in the field, with successful applications in a wide range of tissues in over 50 laboratories worldwide. AtlasXomics has commercialized DBiT-seq for spatial ATAC-seq assays to map chromatin accessibility and is partnering with EpiCypher to introduce spatial CUT&Tag for profiling chromatin proteins, including histone PTMs and transcription factors..

DBiT-seq在该领域迅速获得关注，在全球50多个实验室的广泛组织中成功应用。AtlasXomics已将DBiT-seq商业化用于空间ATAC-seq分析，以绘制染色质可及性，并与EpiCypher合作引入空间切割和标签，用于分析染色质蛋白，包括组蛋白PTM和转录因子。。

'By seamlessly integrating molecular biology with histology, the DBiT-seq platform is revolutionizing epigenomics research,' stated Colin Ng, Vice President of Business Development at AtlasXomics. 'We have developed robust spatial assays that incorporate EpiCypher's proprietary pAG-Tn5 enzyme and high-efficiency antibodies to provide the optimal balance of sensitivity and specificity required for in-depth exploration of histone modifications and chromatin-associated proteins.

AtlasXomics商业发展副总裁柯林·宁（ColinNg）表示：“通过将分子生物学与组织学无缝结合，DBiT-seq平台正在彻底改变表观基因组学研究。”我们已经开发出强大的空间分析方法，该方法结合了EpiCypher专有的pAG-Tn5酶和高效抗体，以提供深入探索组蛋白修饰和染色质相关蛋白所需的灵敏度和特异性的最佳平衡。

Together, we will bring spatial CUT&Tag assays to market, including an expanded set of validated antibodies to provide customers with immediate access to diverse critical targets.'EpiCypher holds key rights to CUT&Tag technology and a proprietary nucleosome-based approach to develop ultra-efficient antibodies for spatial and single cell CUT&Tag assays.

总之，我们将把空间切割和标签检测推向市场，包括一套扩展的经过验证的抗体，以使客户能够立即获得各种关键目标。”EpiCypher拥有CUT＆Tag技术和专有的基于核小体的方法的关键权利，以开发用于空间和单细胞CUT＆Tag分析的超高效抗体。

'Spatial CUT&Tag depends on specific and efficient core reagents to capture more data per cell, which allows deeper biological insights,' said Dr. Martis Cowles, Chief Business Officer at EpiCypher. 'We are very impressed with the DBiT-seq platform and look forward to working with AtlasXomics to maximize its impact on chromatin science and precision medicine.'AtlasXomics and EpiCypher have focused initial development efforts on fresh frozen samples.

EpiCypher首席商务官马蒂斯·考尔斯（MartisCowles）博士说，空间切割和标记取决于特定而高效的核心试剂，以捕获每个细胞更多的数据，从而可以获得更深入的生物学见解我们对DBiT-seq平台印象深刻，期待着与AtlasXomics合作，最大限度地发挥其对染色质科学和精准医学的影响。”AtlasXomics和EpiCypher将初步开发工作集中在新鲜冷冻样品上。

Their long-term goal is to optimize spatial CUT&Tag for formalin-fixed paraffin-embedded (FFPE) samples, thereby greatly enhancing the versatility of these assays for clinical applications.About EpiCypher – EpiCypher was founded in response to the growing demand for high-quality reagents to study chromatin regulation and enable epigenetics-focused drug discovery.

他们的长期目标是优化福尔马林固定石蜡包埋（FFPE）样品的空间切割和标签，从而大大提高这些测定在临床应用中的多功能性。关于EpiCypher–EpiCypher的成立是为了应对对高质量试剂不断增长的需求，以研究染色质调控并实现以表观遗传学为重点的药物发现。

The Company is at the forefront of chromatin mapping technologies with the CUTANA™ platform for ultra-sensitive ChIC, CUT&RUN, and CUT&Tag profi.

该公司在CUTANA染色质作图技术方面处于领先地位™ 超灵敏别致、切割和运行以及切割和标记profi的平台。