Research unveils cost-efficient Flongle sequencing's potential for rapid hantavirus genome-based diagnostics and phylogeographical surveillanceSEOUL, South Korea, Feb. 29, 2024 /PRNewswire/ -- Orthohantaviruses, highly transmissible zoonotic pathogens, are notorious for causing hemorrhagic fever with renal syndrome (HFRS) in Eurasia and hantavirus cardiopulmonary syndrome in the Americas.

研究揭示了具有成本效益的Flongle测序技术在基于汉坦病毒基因组的快速诊断和系统地理学监测方面的潜力。2024年2月29日，韩国首尔/新闻网/--正汉坦病毒是一种高度传播的人畜共患病原体，因在欧亚大陆引起肾综合征出血热（HFRS）和在美洲引起汉坦病毒心肺综合征而臭名昭著。

With a significant impact on public health, they have been extensively researched for effective outbreak control and intervention strategies. Gyeonggi Province in South Korea has reported a significant number of HFRS cases, making it a critical area for epidemiological surveillance and understanding the genomic diversity of orthohantaviruses..

它们对公共卫生产生重大影响，已被广泛研究以有效控制疫情和干预策略。韩国京畿道省报告了大量HFRS病例，使其成为流行病学监测和了解正汉坦病毒基因组多样性的关键领域。。

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Insights from novel HTNV rapid amplicon-based Flongle sequencing helps enhance preparedness in HFRS-endemic areas and contribute to a high-resolution phylogeographical map of Hantaan orthohantavirus

基于新型HTNV快速扩增子的Flongle测序的见解有助于增强HFRS流行地区的防备能力，并有助于绘制汉坦正汉坦病毒的高分辨率系统地理图

To gain insights into the prevalence, viral loads, and genetic variations of Hantaan orthohantavirus (HTNV), a team of researchers led by Prof. Jin-Won Song from the Department of Microbiology, Korea University College of Medicine, conducted a study in Gyeonggi Province. Notably, the study showcased the application of Flongle sequencing, an innovative, cost-efficient, and rapid method, for detecting HTNV genomes.

为了深入了解汉坦正汉坦病毒（HTNV）的流行、病毒载量和遗传变异，韩国大学医学院微生物学系金元松教授领导的一组研究人员在京畿道进行了一项研究。值得注意的是，该研究展示了Flongle测序的应用，这是一种创新，经济高效且快速的方法，用于检测HTNV基因组。

'We developed a rapid and sensitive on-site diagnostic using a nanopore-based Flongle chip with a reasonable cost of around $100. This approach enables virtually whole-genome sequencing of HTNV within 3 hours,' explains Prof. Song. Their work culminated in a research paper, released online on January 4, 2024 and published in Volume 96, Issue 1 of the Journal of Medical Virology in January 2024..

“我们使用基于纳米孔的Flongle芯片开发了一种快速灵敏的现场诊断方法，合理的成本约为100美元。宋教授解释说，这种方法可以在3小时内对HTNV进行几乎全基因组测序。他们的工作以一篇研究论文告终，该论文于2024年1月4日在线发布，并于2024年1月在《医学病毒学杂志》第1期第96卷上发表。。

The study's approach is rooted in the adoption of next-generation sequencing technologies, with a spotlight on the Oxford MinION nanopore sequencer. The comprehensive sampling strategy involved the capture of rodents and shrews from diverse regions in the ROK using live traps. Subsequently, the team employed a multifaceted approach, incorporating mitochondrial DNA analysis, indirect immunofluorescence antibody tests, and various molecular methods for species identification and virus detection.

这项研究的方法植根于采用下一代测序技术，重点是牛津MinION纳米孔测序仪。综合采样策略涉及使用活诱捕器从韩国不同地区捕获啮齿动物和sh。随后，该团队采用了多方面的方法，包括线粒体DNA分析，间接免疫荧光抗体测试以及用于物种鉴定和病毒检测的各种分子方法。

This comprehensive methodology facilitated a thorough understanding of viral prevalence and genetic diversity..

这种全面的方法有助于彻底了解病毒流行和遗传多样性。。

The epidemiological surveillance conducted in Gyeonggi Province from 2017 to 2018 uncovered a significant proportion of Apodemus agrarius, a common rodent species. Notably, 12.4% of A. agrarius displayed seropositivity for HTNV, showcasing the virus's presence in the region. Flongle sequencing proved instrumental in obtaining full-length genomic sequences from positive samples, with impressive coverage rates and accuracy comparable to Illumina sequencing.

2017年至2018年在京畿道进行的流行病学监测发现，很大一部分黑线姬鼠是一种常见的啮齿动物。值得注意的是，12.4%的A.agrarius对HTNV表现出血清阳性，表明该病毒在该地区存在。事实证明，Flongle测序有助于从阳性样品中获得全长基因组序列，其覆盖率和准确性与Illumina测序相当。

The phylogeographical analysis uncovers well-supported evolutionary divergence among HTNV tripartite genomes. Genetic clustering and incongruences in evolutionary patterns emphasize the virus's differential evolution for each genomic segment.Prof. Song emphasizes the significance of this research by stating, 'We believe our findings provide important insights into on-site diagnostics, genome-based surveillance, and the evolutionary dynamics of orthohantaviruses to mitigate hantaviral outbreaks in HFRS-endemic areas in the ROK.

系统地理学分析揭示了HTNV三方基因组之间支持良好的进化分歧。遗传聚类和进化模式的不一致强调了病毒对每个基因组片段的差异进化。宋教授强调了这项研究的重要性，他说：“我们相信我们的发现为现场诊断，基于基因组的监测以及正汉坦病毒的进化动力学提供了重要的见解，以减轻韩国HFRS流行地区的汉坦病毒爆发。

Our study pioneers the integration of cost-efficient Flongle sequencing into hantavirus diagnostics, offering a rapid and accurate tool for on-site detection. This innovation has the potential to transform how we approach and manage hantavirus outbreaks.'While the study provides groundbreaking insights, researchers acknowledge certain limitations, including the need for further sensitivity testing of Flongle-based diagnostics and more extensive genomic and epidemiological data in certain endemic areas.This research marks a significant step forward in our understanding of orthohantaviruses, laying the foundation for more targeted control strategies against HFRS outbreaks.

我们的研究率先将具有成本效益的Flongle测序整合到汉坦病毒诊断中，为现场检测提供了快速准确的工具。这项创新有可能改变我们处理和管理汉坦病毒爆发的方式。”虽然这项研究提供了开创性的见解，但研究人员承认存在某些局限性，包括需要对基于Flongle的诊断进行进一步的敏感性测试，以及在某些流行地区提供更广泛的基因组和流行病学数据。这项研究标志着我们对正汉坦病毒的理解向前迈出了重要一步，为针对HFRS爆发的更有针对性的控制策略奠定了基础。

The findings emphasize the pivotal role of genomics in disease surveillance and offer hope for more effective responses to emer.

这些发现强调了基因组学在疾病监测中的关键作用，并为更有效地应对emer提供了希望。