l-阿拉伯糖代谢与毒力调节相结合促进肠道病原体适应性-动脉网

Metabolism of l-arabinose converges with virulence regulation to promote enteric pathogen fitness

Nature 等信源发布 2024-05-25 20:16



可切换为仅中文







AbstractVirulence and metabolism are often interlinked to control the expression of essential colonisation factors in response to host-associated signals. Here, we identified an uncharacterised transporter of the dietary monosaccharide ʟ-arabinose that is widely encoded by the zoonotic pathogen enterohaemorrhagic Escherichia coli (EHEC), required for full competitive fitness in the mouse gut and highly expressed during human infection.

摘要毒力和代谢通常是相互关联的，以控制响应宿主相关信号的必需定植因子的表达。在这里，我们确定了膳食单糖ʟ-阿拉伯糖的未表征转运蛋白，该转运蛋白由人畜共患病原体肠出血性大肠杆菌（EHEC）广泛编码，是小鼠肠道完全竞争适应性所必需的，并且在人类感染期间高度表达。

Discovery of this transporter suggested that EHEC strains have an enhanced ability to scavenge ʟ-arabinose and therefore prompted us to investigate the impact of this nutrient on pathogenesis. Accordingly, we discovered that ʟ-arabinose enhances expression of the EHEC type 3 secretion system, increasing its ability to colonise host cells, and that the underlying mechanism is dependent on products of its catabolism rather than the sensing of ʟ-arabinose as a signal.

这种转运蛋白的发现表明EHEC菌株具有增强的清除β-阿拉伯糖的能力，因此促使我们研究这种营养素对发病机制的影响。因此，我们发现ʟ-阿拉伯糖增强了EHEC 3型分泌系统的表达，增加了其定殖宿主细胞的能力，并且其潜在机制取决于其分解代谢产物，而不是将ʟ-阿拉伯糖感知为信号。

Furthermore, using the murine pathogen Citrobacter rodentium, we show that ʟ-arabinose metabolism provides a fitness benefit during infection via virulence factor regulation, as opposed to supporting pathogen growth. Finally, we show that this mechanism is not restricted to ʟ-arabinose and extends to other pentose sugars with a similar metabolic fate.

此外，使用鼠病原体柠檬酸杆菌（Citrobacter rodentium），我们表明ʟ-阿拉伯糖代谢通过毒力因子调节在感染期间提供了适应性益处，而不是支持病原体的生长。最后，我们表明，这种机制不仅限于ʟ-阿拉伯糖，还扩展到其他具有类似代谢命运的戊糖。

This work highlights the importance integrating central metabolism with virulence regulation in order to maximise competitive fitness of enteric pathogens within the host-niche..

这项工作强调了将中枢代谢与毒力调节相结合的重要性，以最大限度地提高肠道病原体在宿主生态位内的竞争适应性。。

IntroductionThe mammalian gastrointestinal tract poses a formidable barrier to infection by foreign pathogens. Invaders must sidestep a combination of intrinsic host defences as well as overcome colonisation resistance by the native gut microbiota1. Accordingly, bacterial pathogens have evolved many unique strategies to effectively compete with the microbiota and cause infection within a favourable host-niche2.

引言哺乳动物胃肠道对外来病原体的感染构成了强大的屏障。入侵者必须避开内在宿主防御的组合，并克服天然肠道微生物群的定殖阻力1。因此，细菌病原体已经发展出许多独特的策略来有效地与微生物群竞争并在有利的宿主-niche2内引起感染。

This includes a combination of pathogen-specific virulence mechanisms and metabolic adaptations that increase within-host fitness. Importantly, virulence and fitness factor regulation often coincide and are dynamically controlled in response to the host environment to maximise competitiveness therein3,4,5.Enterohaemorrhagic Escherichia coli (EHEC) is a zoonotic pathogen that is carried asymptomatically by ruminant mammals and transmitted to human hosts typically via contaminated meat and fresh produce.

这包括病原体特异性毒力机制和在宿主适应性内增加的代谢适应的组合。重要的是，毒力和适应因子的调节通常是一致的，并根据宿主环境进行动态控制，以最大程度地提高其竞争力3,4,5。肠出血性大肠杆菌（EHEC）是一种人畜共患病原体，由反刍哺乳动物无症状携带，通常通过受污染的肉类和新鲜农产品传播给人类宿主。

In humans, EHEC causes severe diarrhoeal illness and, in extreme cases, renal failure6,7. EHEC is a member of the attaching and effacing (A/E) family of pathogens, which intimately colonise the colonic epithelium forming characteristic pedestal-like lesions on the surface of host cells8,9. A/E pathogenesis is defined by the activity of a type 3 secretion system (T3SS) encoded on a ~35 kb horizontally acquired island known as the locus of enterocyte effacement (LEE)10,11,12.

在人类中，肠出血性大肠杆菌会导致严重的腹泻病，在极端情况下还会导致肾衰竭6,7。EHEC是附着和消除（a/E）病原体家族的成员，其紧密定植于结肠上皮，在宿主细胞表面形成特征性的基座状病变8,9。A/E发病机制由〜35编码的3型分泌系统（T3SS）的活性定义 kb水平获得的岛被称为肠上皮细胞消失（LEE）的基因座10,11,12。

This T3SS translocates more than 30 effector proteins - encoded on the LEE and several additional horizontally acquired elements, termed O-islands (OIs) - that collectively subvert host-cell function13,14,15,16. The murine pathogen Citrobacter rodentium also encodes the LEE and has been adopted as the relevant surrogate model to study EHEC pathogenesis in vivo due to its dependency on the T3SS, .

该T3SS易位超过30种效应蛋白-编码在LEE上和另外几个水平获得的元件，称为O岛（OIs）-共同破坏宿主细胞功能13,14,15,16。鼠类病原体柠檬酸杆菌（Citrobacter rodentium）也编码LEE，由于其对T3SS的依赖性，已被用作研究体内EHEC发病机理的相关替代模型。

Data availability

数据可用性

The transcriptomic sequencing data is available from the NCBI Gene Expression Omnibus under the accession number GSE262155. The source data for Figs. 2–6, Supplementary Figs. 3–5 and 7–12 are provided as a Source Data file. Publicly available genome sequences were obtained from the NCBI Sequence Read Archive. Source data are provided with this paper..

转录组测序数据可从NCBI Gene Expression Omnibus获得，登录号为GSE262155。图2-6、补充图3-5和7-12的源数据作为源数据文件提供。公开可用的基因组序列是从NCBI序列读取档案中获得的。本文提供了源数据。。

ReferencesCaballero-Flores, G., Pickard, J. M. & Núñez, G. Microbiota-mediated colonization resistance: mechanisms and regulation. Nat. Rev. Microbiol. 21, 347–360 (2023).Article

参考文献Caballero-Flores，G.，Pickard，J.M。＆Núñez，G。微生物群介导的定植抗性：机制和调节。自然修订版微生物学。21347-360（2023）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Baümler, A. J. & Sperandio, V. Interactions between the microbiota and pathogenic bacteria in the gut. Nature 535, 85–93 (2016).Article

Baümler，A.J。＆Sperandio，V。肠道微生物群与病原菌之间的相互作用。《自然》535,85-93（2016）。文章

ADS

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Connolly, J. P. R., Brett Finlay, B. & Roe, A. J. From ingestion to colonization: the influence of the host environment on regulation of the LEE encoded type III secretion system in enterohaemorrhagic Escherichia coli. Front. Microbiol. 6, 568 (2015).Article

Connolly，J.P.R.，Brett-Finlay，B。＆Roe，A.J。从摄入到定植：宿主环境对肠出血性大肠杆菌中LEE编码的III型分泌系统调节的影响。正面。微生物。6568（2015）。文章

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Turner, N. C. A., Connolly, J. P. R. & Roe, A. J. Control freaks—signals and cues governing the regulation of virulence in attaching and effacing pathogens. Biochem. Soc. Trans. 47, 229–238 (2018).Article

Turner，N.C.A.，Connolly，J.P.R。＆Roe，A.J。控制畸形信号和线索，控制附着和消除病原体的毒力调节。生物化学。社会事务。47229-238（2018）。文章

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Wale, K. R., Cottam, C., Connolly, J. P. & Roe, A. J. Transcriptional and metabolic regulation of EHEC and Citrobacter rodentium pathogenesis. Curr. Opin. Microbiol. 63, 70–75 (2021).Article

Wale，K.R.，Cottam，C.，Connolly，J.P。＆Roe，A.J。EHEC和柠檬酸杆菌发病机制的转录和代谢调控。货币。奥平。微生物。63，70-75（2021）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Kaper, J. B., Nataro, J. P. & Mobley, H. L. Pathogenic Escherichia coli. Nat. Rev. Microbiol. 2, 123–140 (2004).Article

Kaper，J.B.，Nataro，J.P。＆Mobley，H.L。致病性大肠杆菌。自然修订版微生物学。2123-140（2004）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Croxen, M. A. & Finlay, B. B. Molecular mechanisms of Escherichia coli pathogenicity. Nat. Rev. Microbiol. 8, 26–38 (2010).Article

Croxen，M.A。＆Finlay，B.B。大肠杆菌致病性的分子机制。自然修订版微生物学。8，26-38（2010）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Moon, H. W., Whipp, S. C., Argenzio, R. A., Levine, M. M. & Giannella, R. A. Attaching and effacing activities of rabbit and human enteropathogenic Escherichia coli in pig and rabbit intestines. Infect. Immun. 41, 1340–1351 (1983).Article

Moon，H.W.，Whipp，S.C.，Argenzio，R.A.，Levine，M.M。和Giannella，R.A。猪和兔肠中兔和人肠致病性大肠杆菌的附着和消除活性。感染。免疫。411340-1351（1983）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Kenny, B. et al. Enteropathogenic E. coli (EPEC) transfers its receptor for intimate adherence into mammalian cells. Cell 91, 511–520 (1997).Article

肠道致病性大肠杆菌（EPEC）将其受体转移到哺乳动物细胞中，使其紧密粘附。细胞91511-520（1997）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Jerse, A. E., Yu, J., Tall, B. D. & Kaper, J. B. A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proc. Natl Acad. Sci. USA 87, 7839–7843 (1990).Article

Jerse，A.E.，Yu，J.，Tall，B.D。＆Kaper，J.B。肠致病性大肠杆菌的遗传位点，是在组织培养细胞上产生附着和消除病变所必需的。程序。国家科学院。科学。美国877839-7843（1990）。文章

ADS

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Mcdaniel, T. K., Jarvis, K. G., Donnenberg, M. S. & Kaper, J. B. A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens. Proc. Natl Acad. Sci. USA 92, 1664–1668 (1995).Article

Mcdaniel，T.K.，Jarvis，K.G.，Donnenberg，M.S。＆Kaper，J.B。一种在多种肠道细菌病原体中保守的肠细胞消失的遗传基因座。程序。国家科学院。科学。美国921664-1668（1995）。文章

ADS

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Deng, W. et al. Dissecting virulence: systematic and functional analyses of a pathogenicity island. Proc. Natl Acad. Sci. USA 101, 3597–3602 (2004).Article

Deng，W。等人。解剖毒力：致病岛的系统和功能分析。程序。国家科学院。科学。美国1013597-3602（2004）。文章

ADS

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Dean, P. & Kenny, B. The effector repertoire of enteropathogenic E. coli: ganging up on the host cell. Curr. Opin. Microbiol. 12, 101–109 (2009).Article

Dean，P。＆Kenny，B。肠致病性大肠杆菌的效应库：在宿主细胞上聚集。货币。奥平。微生物。12101-109（2009）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Tobe, T. et al. An extensive repetoire of type III secretion effectors in Escherichia coli O157 and the role of lambdoid phages in their dissemination. Proc. Natl Acad. Sci. USA 103, 14941–14946 (2006).Article

Tobe，T。等人。大肠杆菌O157中III型分泌效应子的广泛重复以及lambdoid噬菌体在其传播中的作用。程序。国家科学院。科学。美国10314941–14946（2006）。文章

ADS

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Perna, N. T. et al. Genome sequence of enterohaemorrhagic Escherichia coli O157:H7. Nature 409, 529–533 (2001).Article

Perna，N.T.等人。肠出血性大肠杆菌O157:H7的基因组序列。自然409529-533（2001）。文章

ADS

CAS

中科院

PubMed

Google Scholar

谷歌学者

Wong, A. R. C. et al. Enteropathogenic and enterohaemorrhagic Escherichia coli: Even more subversive elements. Mol. Microbiol. 80, 1420–1438 (2011).Article

Wong，A.R.C.等人，《肠致病性和肠出血性大肠杆菌：更具颠覆性的元素》。分子微生物。801420-1438（2011）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Collins, J. W. et al. Citrobacter rodentium: Infection, inflammation and the microbiota. Nat. Rev. Microbiol. 12, 612–623 (2014).Article

Collins，J.W.等人，《啮齿动物柠檬酸杆菌：感染，炎症和微生物群》。自然修订版微生物学。12612-623（2014）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Mullineaux-Sanders, C. et al. Citrobacter rodentium–host–microbiota interactions: immunity, bioenergetics and metabolism. Nat. Rev. Microbiol. 17, 701–715 (2019).Article

Mullineaux Sanders，C.等人，《柠檬酸杆菌-宿主-微生物群的相互作用：免疫，生物能学和代谢》。自然修订版微生物学。17701-715（2019）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Crepin, V. F., Collins, J. W., Habibzay, M. & Frankel, G. Citrobacter rodentium mouse model of bacterial infection. Nat. Protoc. 11, 1851–1876 (2016).Article

Crepin，V.F.，Collins，J.W.，Habibzay，M。＆Frankel，G.柠檬酸杆菌啮齿动物细菌感染小鼠模型。自然协议。111851-1876（2016）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Kamada, N. et al. Regulated virulence controls the ability of a pathogen to compete with the gut microbiota. Science 336, 1325–1329 (2012).Article

Kamada，N。等人调节毒力控制病原体与肠道微生物群竞争的能力。《科学》3361325–1329（2012）。文章

ADS

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Connolly, J. P. R. et al. Host-associated niche metabolism controls enteric infection through fine-tuning the regulation of type 3 secretion. Nat. Commun. 9, 4187 (2018).Article

Connolly，J.P.R。等人。宿主相关的生态位代谢通过微调3型分泌的调节来控制肠道感染。国家公社。94187（2018）。文章

ADS

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Njoroge, J. W., Nguyen, Y., Curtis, M. M., Moreira, C. G. & Sperandio, V. Virulence meets metabolism: Cra and KdpE gene regulation in enterohemorrhagic Escherichia coli. MBio 3, e00280–00212 (2012).Article

Njoroge，J.W.，Nguyen，Y.，Curtis，M.M.，Moreira，C.G。＆Sperandio，V。毒力满足代谢：肠出血性大肠杆菌中的Cra和KdpE基因调控。MBio 3，e00280–00212（2012）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Curtis, M. M. et al. The gut commensal bacteroides thetaiotaomicron exacerbates enteric infection through modification of the metabolic landscape. Cell Host Microbe 16, 759–769 (2014).Article

Curtis，M.M.等人。肠道共生类杆菌thetaiotaomicron通过改变代谢景观加剧肠道感染。细胞宿主微生物16759-769（2014）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Menezes-Garcia, Z., Kumar, A., Zhu, W., Winter, S. E. & Sperandio, V. L-Arginine sensing regulates virulence gene expression and disease progression in enteric pathogens. Proc. Natl Acad. Sci. USA 117, 12387–12393 (2020).Article

Menezes-Garcia，Z.，Kumar，A.，Zhu，W.，Winter，S.E。＆Sperandio，V.L-精氨酸感应调节肠道病原体的毒力基因表达和疾病进展。程序。国家科学院。科学。美国11712387–12393（2020）。文章

ADS

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Connolly, J. P. R. et al. The host metabolite D-serine contributes to bacterial niche specificity through gene selection. ISME J. 9, 1039–1051 (2015).Article

Connolly，J.P.R.等人。宿主代谢物D-丝氨酸通过基因选择促进细菌生态位特异性。ISME J.91039–1051（2015）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

O’Boyle, N., Connolly, J. P. R., Tucker, N. P. & Roe, A. J. Genomic plasticity of pathogenic Escherichia coli mediates D-serine tolerance via multiple adaptive mechanisms. Proc. Natl Acad. Sci. USA 117, 22484–22493 (2020).Article

O'Boyle，N.，Connolly，J.P.R.，Tucker，N.P。＆Roe，A.J。致病性大肠杆菌的基因组可塑性通过多种适应机制介导D-丝氨酸耐受性。程序。国家科学院。科学。美国11722484–22493（2020）。文章

ADS

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Conway, T. & Cohen, P. S. Commensal and pathogenic Escherichia coli Metabolism in the gut. Microbiol. Spectr. 3, MBP-0006-2014 (2015).Article

Conway，T。＆Cohen，P。S。肠道中的共生和致病性大肠杆菌代谢。微生物。光谱。3，MBP-0006-2014（2015）。文章

Google Scholar

谷歌学者

Fabich, A. J. et al. Comparison of carbon nutrition for pathogenic and commensal Escherichia coli strains in the mouse intestine. Infect. Immun. 76, 1143–1152 (2008).Article

Fabich，A.J.等人。小鼠肠道中致病性和共生大肠杆菌菌株的碳营养比较。感染。免疫。761143-1152（2008）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Crozier, L. et al. The role of l-arabinose metabolism for Escherichia coli o157:H7 in edible plants. Microbiology 167, 1–12 (2021).Article

Crozier，L。等人。食用植物中大肠杆菌o157:H7的L-阿拉伯糖代谢的作用。微生物学167,1-12（2021）。文章

Google Scholar

谷歌学者

Mayer, C. & Boos, W. Hexose/pentose and hexitol/pentitol metabolism. EcoSal Plus https://doi.org/10.1128/ecosalplus.3.4.1 (2005).John, M. et al. Use of in vivo-induced antigen technology for identification of Escherichia coli O157:H7 proteins expressed during human infection. Infect.

Mayer，C。＆Boos，W。己糖/戊糖和己糖醇/奔腾醇代谢。EcoSal Plushttps://doi.org/10.1128/ecosalplus.3.4.1（2005年）。John，M.等人。使用体内诱导抗原技术鉴定人类感染期间表达的大肠杆菌O157:H7蛋白。感染。

Immun. 73, 2665–2679 (2005).Article .

有免疫力73, 2665-2679 (2005).第[UNK]条。

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Segura, A. et al. Transcriptomic analysis reveals specific metabolic pathways of enterohemorrhagic Escherichia coli O157:H7 in bovine digestive contents. BMC Genom. 19, 766 (2018).Article

Segura，A。等人。转录组学分析揭示了肠出血性大肠杆菌O157:H7在牛消化内容物中的特定代谢途径。BMC基因组。19766（2018）。文章

Google Scholar

谷歌学者

Rice, A. J., Park, A. & Pinkett, H. W. Diversity in ABC transporters: type I, II and III importers. Crit. Rev. Biochem. Mol. Biol. 49, 426–437 (2014).Article

Rice，A.J.，Park，A。＆Pinkett，H.W。ABC运输商的多样性：I型，II型和III型进口商。暴击。生物化学评论。分子生物学。49426-437（2014）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Drousiotis, K. et al. Characterization of the l-arabinofuranose-specific GafABCD ABC transporter essential for l-arabinose-dependent growth of the lignocellulose-degrading bacterium Shewanella sp. ANA-3. Microbiology 169, 3 (2023).Article

Drousiotis，K。等人。木质纤维素降解细菌Shewanella sp。ANA-3的l-阿拉伯糖依赖性生长所必需的l-阿拉伯呋喃糖特异性GafABCD ABC转运蛋白的表征。微生物学169,3（2023）。文章

Google Scholar

谷歌学者

Zschiedrich, C. P., Keidel, V. & Szurmant, H. Molecular mechanisms of two-component signal transduction. J. Mol. Biol. 428, 3752–3775 (2016).Article

Zschiedrich，C.P.，Keidel，V。＆Szurmant，H。双组分信号转导的分子机制。J、分子生物学。4283752-3775（2016）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Schleif, R. AraC protein, regulation of the l-arabinose operon in Escherichia coli, and the light switch mechanism of AraC action. FEMS Microbiol. Rev. 34, 779–796 (2010).Article

Schleif，R。AraC蛋白，大肠杆菌中l-阿拉伯糖操纵子的调节以及AraC作用的光开关机制。FEMS微生物。第34779-796版（2010年）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Mullineaux-Sanders, C. et al. Citrobacter rodentium relies on commensals for colonization of the colonic mucosa. Cell Rep. 21, 3381–3389 (2017).Article

Mullineaux-Sanders，C。等人。啮齿动物柠檬酸杆菌依赖共生体来定殖结肠粘膜。Cell Rep.213381–3389（2017）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Carlson-Banning, K. M. & Sperandio, V. Catabolite and oxygen regulation of enterohemorrhagic Escherichia coli virulence. MBio 7, e01852–16 (2016).Article

Carlson Banning，K.M。＆Sperandio，V。肠出血性大肠杆菌毒力的分解代谢和氧调节。MBio 7，e01852–16（2016）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Freter, R., Brickner, H., Fekete, J., Vickerman, M. M. & Carey, K. E. Survival and implantation of Escherichia coli in the intestinal tract. Infect. Immun. 39, 686–703 (1983).Article

Freter，R.，Brickner，H.，Fekete，J.，Vickerman，M.M。＆Carey，K.E。大肠杆菌在肠道中的存活和植入。感染。免疫。39686-703（1983）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Yip, A. Y. G. et al. Antibiotics promote intestinal growth of carbapenem-resistant Enterobacteriaceae by enriching nutrients and depleting microbial metabolites. Nat. Commun. 14, 5094 (2023).Article

Yip，A.Y.G.等人。抗生素通过富集营养物质和消耗微生物代谢物来促进碳青霉烯类耐药肠杆菌科的肠道生长。国家公社。145094（2023）。文章

ADS

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Liu, B. et al. Enterohaemorrhagic E. coli utilizes host- and microbiota-derived L-malate as a signaling molecule for intestinal colonization. Nat. Commun. 14, 7227 (2023).Article

Liu，B。等人。肠出血性大肠杆菌利用宿主和微生物群衍生的L-苹果酸作为肠道定植的信号分子。国家公社。147227（2023）。文章

ADS

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Schutte, J. B., de Jong, J., van Weerden, E. J. & Tamminga, S. Nutritional implications of l -arabinose in pigs. Br. J. Nutr. 68, 195–207 (1992).Article

Schutte，J.B.，DeJong，J.，van Weerden，E.J。＆Tamminga，S。l-阿拉伯糖对猪的营养意义。英国营养学杂志。68195-207（1992）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Schwalm, N. D., Townsend, G. E. & Groisman, E. A. Multiple signals govern utilization of a polysaccharide in the gut bacterium bacteroides thetaiotaomicron. MBio https://doi.org/10.1128/mbio.01342-16 (2016).Pereira, G. V. et al. Degradation of complex arabinoxylans by human colonic Bacteroidetes.

Schwalm，N.D.，Townsend，G.E。和Groisman，E.A。多种信号控制肠道细菌bacteroides thetaiotaomicron中多糖的利用。MBio公司https://doi.org/10.1128/mbio.01342-16（2016年）。Pereira，G.V.等人。人类结肠拟杆菌对复杂阿拉伯木聚糖的降解。

Nat. Commun. 12, 459 (2021).Article .

Nat.普通。12459（2021）。文章。

ADS

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Martens, E. C. et al. Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts. PLoS Biol. 9, e1001221 (2011).Article

Martens，E.C.等人。两种人类肠道共生体对植物细胞壁多糖的识别和降解。《公共科学图书馆·生物学》。9，e1001221（2011）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Rogowski, A. et al. Glycan complexity dictates microbial resource allocation in the large intestine. Nat. Commun. 6, 7481 (2015).Article

Rogowski，A。等人。聚糖的复杂性决定了大肠中微生物资源的分配。国家公社。67481（2015）。文章

ADS

CAS

中科院

PubMed

Google Scholar

谷歌学者

Kyle, J. L., Parker, C. T., Goudeau, D. & Brandl, M. T. Transcriptome analysis of escherichia coli O157:H7 exposed to lysates of lettuce leaves. Appl. Environ. Microbiol. 76, 1375–1387 (2010).Article

Kyle，J.L.，Parker，C.T.，Goudeau，D。和Brandl，M.T。暴露于莴苣叶裂解物的大肠杆菌O157:H7的转录组分析。应用。环境。微生物。761375-1387（2010）。文章

ADS

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Petty, N. K. et al. The Citrobacter rodentium genome sequence reveals convergent evolution with human pathogenic Escherichia coli. J. Bacteriol. 192, 525–538 (2010).Article

啮齿动物柠檬酸杆菌基因组序列揭示了与人类致病性大肠杆菌的趋同进化。J、细菌。192525-538（2010）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Harper, L. et al. Staphylococcus aureus responds to the central metabolite pyruvate to regulate virulence. MBio https://doi.org/10.1128/mbio.02272-17 (2018).Jiang, L. et al. Salmonella Typhimurium reprograms macrophage metabolism via T3SS effector SopE2 to promote intracellular replication and virulence.

Harper，L。等人。金黄色葡萄球菌对中枢代谢物丙酮酸有反应以调节毒力。MBio公司https://doi.org/10.1128/mbio.02272-17（2018年）。Jiang，L。等人。鼠伤寒沙门氏菌通过T3SS效应子SopE2重编程巨噬细胞代谢，以促进细胞内复制和毒力。

Nat. Commun. 12, 879 (2021).Article .

Nat.普通。12879（2021）。文章。

ADS

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Wiebe, M. A. et al. Serine deamination is a new acid tolerance mechanism observed in uropathogenic Escherichia coli. MBio 13, e02963-22 (2022).Article

Wiebe，M.A.等人。丝氨酸脱氨是在尿路致病性大肠杆菌中观察到的一种新的耐酸机制。MBio 13，e02963-22（2022）。文章

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Ruddle, S. J., Massis, L. M., Cutter, A. C. & Monack, D. M. Salmonella-liberated dietary L-arabinose promotes expansion in superspreaders. Cell Host Microbe 31, 405–417.e5 (2023).Article

Ruddle，S.J.，Massis，L.M.，Cutter，A.C。和Monack，D.M。沙门氏菌释放的膳食L-阿拉伯糖促进了超级扩张器的扩张。细胞宿主微生物31405–417.e5（2023）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Neumann, M. et al. Deprivation of dietary fiber in specific-pathogen-free mice promotes susceptibility to the intestinal mucosal pathogen Citrobacter rodentium. Gut Microbes 13, 1966263 (2021).Article

Neumann，M.等人。在无特定病原体的小鼠中剥夺膳食纤维会促进对肠粘膜病原体柠檬酸杆菌的易感性。肠道微生物131966263（2021）。文章

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Desai, M. S. et al. A dietary fiber-deprived gut microbiota degrades the colonic mucus barrier and enhances pathogen susceptibility. Cell 167, 1339–1353.e21 (2016).Article

Desai，M.S.等人。缺乏膳食纤维的肠道微生物群会降解结肠粘液屏障并增强病原体的易感性。细胞1671339-1353.e21（2016）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

An, J. et al. Western-style diet impedes colonization and clearance of Citrobacter rodentium. PLoS Pathog. 17, e1009497 (2021).Article

An，J.等人。西式饮食阻碍了柠檬酸杆菌的定殖和清除。PLoS Pathog。17，e1009497（2021）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Jimenez, A. G., Ellermann, M., Abbott, W. & Sperandio, V. Diet-derived galacturonic acid regulates virulence and intestinal colonization in enterohaemorrhagic Escherichia coli and Citrobacter rodentium. Nat. Microbiol. 5, 368–378 (2020).Article

Jimenez，A.G.，Ellermann，M.，Abbott，W。＆Sperandio，V。饮食衍生的半乳糖醛酸调节肠出血性大肠杆菌和柠檬酸杆菌的毒力和肠道定植。自然微生物。5368-378（2020）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Datsenko, K. A. & Wanner, B. L. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl Acad. Sci. USA 97, 6640–6645 (2000).Article

Datsenko，K.A。＆Wanner，B.L。使用PCR产物一步灭活大肠杆菌K-12中的染色体基因。程序。国家科学院。科学。美国976640–6645（2000）。文章

ADS

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Livak, K. J. & Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 25, 402–408 (2001).Article

Livak，K.J。＆Schmittgen，T.D。使用实时定量PCR和2-ΔΔCT方法分析相对基因表达数据。方法25402-408（2001）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Patro, R., Duggal, G., Love, M. I., Irizarry, R. A. & Kingsford, C. Salmon provides fast and bias-aware quantification of transcript expression. Nat. Methods 14, 417–419 (2017).Article

Patro，R.，Duggal，G.，Love，M.I.，Irizarry，R.A。＆Kingsford，C.Salmon提供了快速且有偏见的转录本表达定量。自然方法14417-419（2017）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Love, M. I., Huber, W. & Anders, S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 15, 550 (2014).Article

Love，M.I.，Huber，W。＆Anders，S。用DESeq2缓和了RNA-seq数据的倍数变化和分散估计。基因组生物学。15550（2014）。文章

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Szklarczyk, D. et al. The STRING database in 2021: customizable protein-protein networks, and functional characterization of user-uploaded gene/measurement sets. Nucleic Acids Res. 49, D605–D612 (2021).Article

Szklarczyk，D。等人。2021年的STRING数据库：可定制的蛋白质-蛋白质网络，以及用户上传的基因/测量集的功能表征。核酸研究49，D605–D612（2021）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Ewels, P., Magnusson, M., Lundin, S. & Käller, M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics 32, 3047–3048 (2016).Article

Ewels，P.，Magnusson，M.，Lundin，S.＆Käller，M.MultiQC：在一份报告中总结多种工具和样品的分析结果。生物信息学323047-3048（2016）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Wood, D. E. & Salzberg, S. L. Kraken: ultrafast metagenomic sequence classification using exact alignments. Genome Biol. 15, R46 (2014).Article

Wood，D.E。＆Salzberg，S.L.Kraken：使用精确比对的超快宏基因组序列分类。基因组生物学。15，R46（2014）。文章

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Bolger, A. M., Lohse, M. & Usadel, B. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 30, 2114–2120 (2014).Article

Bolger，A.M.，Lohse，M。和Usadel，B。Trimmomatic：用于Illumina序列数据的柔性修剪器。生物信息学302114-2120（2014）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Huang, W., Li, L., Myers, J. R. & Marth, G. T. ART: a next-generation sequencing read simulator. Bioinformatics 28, 593–594 (2012).Article

Huang，W.，Li，L.，Myers，J.R。和Marth，G.T。ART：下一代测序读取模拟器。生物信息学28593-594（2012）。文章

PubMed

Google Scholar

谷歌学者

Li, H. & Durbin, R. Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics 25, 1754–1760 (2009).Article

Li，H。＆Durbin，R。使用Burrows-Wheeler变换进行快速准确的短读比对。生物信息学251754-1760（2009）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Li, H. et al. The sequence alignment/map format and SAMtools. Bioinformatics 25, 2078–2079 (2009).Article

Li，H。等人。序列比对/图谱格式和SAMtools。生物信息学252078-2079（2009）。文章

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

McKenna, A. et al. The genome analysis toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data. Genome Res. 20, 1297–1303 (2010).Article

McKenna，A.等人，《基因组分析工具包：用于分析下一代DNA测序数据的MapReduce框架》。基因组研究201297-1303（2010）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Depristo, M. A. et al. A framework for variation discovery and genotyping using next-generation DNA sequencing data. Nat. Genet. 43, 491–501 (2011).Article

Depristo，M.A。等人。使用下一代DNA测序数据进行变异发现和基因分型的框架。纳特·吉内特。43491-501（2011）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Quinlan, A. R. & Hall, I. M. BEDTools: a flexible suite of utilities for comparing genomic features. Bioinformatics 26, 841–842 (2010).Article

Quinlan，A.R。＆Hall，I.M。BEDTools：用于比较基因组特征的灵活实用程序套件。生物信息学26841-842（2010）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Cingolani, P. et al. A program for annotating and predicting the effects of single nucleotide polymorphisms, SnpEff: SNPs in the genome of Drosophila melanogaster strain w1118; iso-2; iso-3. Fly 6, 80–92 (2012).Article

Cingolani，P。等人。注释和预测单核苷酸多态性影响的程序，SnpEff：黑腹果蝇w1118株基因组中的SNP；iso-2；iso-3。Fly 6，80–92（2012）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Sarovich, D. S. & Price, E. P. SPANDx: a genomics pipeline for comparative analysis of large haploid whole genome re-sequencing datasets. BMC Res. Notes 7, 618 (2014).Song, L., Florea, L. & Langmead, B. Lighter: fast and memory-efficient sequencing error correction without counting. Genome Biol.

Sarovich，D.S.＆Price，E.P.SPANDx：用于比较分析大型单倍体全基因组重测序数据集的基因组学管道。BMC Res.注释7618（2014）。Song，L.，Florea，L。＆Langmead，B。Lighter：快速且内存高效的测序错误校正，无需计数。基因组生物学。

15, 509 (2014).Article .

15509（2014）。文章。

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Magoč, T. & Salzberg, S. L. FLASH: fast length adjustment of short reads to improve genome assemblies. Bioinformatics 27, 2957–2963 (2011).Article

Magoč，T。＆Salzberg，S.L。FLASH：快速调整短读数的长度以改善基因组组装。生物信息学272957–2963（2011）。文章

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Bankevich, A. et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J. Comput. Biol. 19, 455–477 (2012).Article

Bankevich，A。et al。SPAdes：一种新的基因组组装算法及其在单细胞测序中的应用。J、计算机。生物学19455-477（2012）。文章

MathSciNet

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Walker, B. J. et al. Pilon: an integrated tool for comprehensive microbial variant detection and genome assembly improvement. PLoS ONE 9, e112963 (2014).Article

Walker，B.J.等人。Pilon：用于全面微生物变异检测和基因组组装改进的综合工具。PLoS ONE 9，e112963（2014）。文章

ADS

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Gurevich, A., Saveliev, V., Vyahhi, N. & Tesler, G. QUAST: quality assessment tool for genome assemblies. Bioinformatics 29, 1072–1075 (2013).Article

Gurevich，A.，Saveliev，V.，Vyahhi，N。＆Tesler，G。QUAST：基因组组装的质量评估工具。生物信息学291072-1075（2013）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Larsen, M. V. et al. Multilocus sequence typing of total-genome-sequenced bacteria. J. Clin. Microbiol. 50, 1355–1361 (2012).Article

Larsen，M.V。等人。全基因组测序细菌的多位点序列分型。临床杂志。微生物。501355-1361（2012）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Stamatakis, A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30, 1312–1313 (2014).Article

Stamatakis，A。RAxML版本8：用于系统发育分析和大型系统发育后分析的工具。生物信息学301312-1313（2014）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Jumper, J. et al. Highly accurate protein structure prediction with AlphaFold. Nature 596, 583–589 (2021).Article

Jumper，J.等人。使用AlphaFold进行高度准确的蛋白质结构预测。自然596583-589（2021）。文章

ADS

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Evans, R. et al. Protein complex prediction with AlphaFold-Multimer. Preprint at bioRxiv https://doi.org/10.1101/2021.10.04.463034 (2022).Edgar, R. C. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 32, 1792–1797 (2004).Article

Evans，R。等人。使用AlphaFold多聚体预测蛋白质复合物。bioRxiv预印本https://doi.org/10.1101/2021.10.04.463034（2022年）。Edgar，R.C.MUSCLE：具有高精度和高通量的多序列比对。核酸研究321792-1797（2004）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol. 35, 1547–1549 (2018).Article

Kumar，S.，Stecher，G.，Li，M.，Knyaz，C。和Tamura，K。MEGA X：跨计算平台的分子进化遗传学分析。分子生物学。进化。351547-1549（2018）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Download referencesAcknowledgementsWe are very grateful to Dr Nicky O’Boyle, Dr Kate Beckham and Dr Kesha Josts for their critical appraisal of the work. We also would like to thank Professor Janet Quinn for microscope access, the Comparative Biology Centre staff for their support with the animal studies and the Newcastle University Genomics Core Facility for RNA-sequencing.

下载参考文献致谢我们非常感谢Nicky O'Boyle博士，Kate Beckham博士和Kesha Josts博士对这项工作的批判性评估。我们还要感谢Janet Quinn教授的显微镜访问，比较生物学中心工作人员对动物研究的支持以及纽卡斯尔大学基因组学RNA测序核心设施。

This work was supported by a Springboard Award from the Academy of Medical Sciences/Wellcome Trust [SBF005\1029], a Royal Society Research Grant [RGS\R2\202100], a Medical Research Council Career Development Award [MR/X007197/1] and a Faculty Fellowship (Newcastle University) awarded to J.P.R.C. E.C.L was supported by a Wellcome Trust Discovery Award [226644/Z/22/Z].

这项工作得到了医学科学院/惠康基金会的跳板奖[SBF005-1029]，皇家学会研究基金[RGS-202100]，医学研究委员会职业发展奖[MR/X007197/1]和授予J.P.R.C.的教师奖学金（纽卡斯尔大学）的支持。E.C.L得到了惠康基金会发现奖[226644/Z/22/Z]的支持。

C.C. was supported by a PhD studentship from the Barbour Foundation.Author informationAuthors and AffiliationsNewcastle University Biosciences Institute, Newcastle University, NE2 4HH, Newcastle-upon-Tyne, UKCurtis Cottam, Elisabeth C. Lowe & James P. R. ConnollyInstitute of Environmental Science and Research, Wellington, New ZealandRhys T.

C、 C.得到了巴伯基金会的博士生资助。作者信息作者和附属机构纽卡斯尔大学生物科学研究所，纽卡斯尔大学，NE2 4HH，泰恩河畔纽卡斯尔，UKCurtis Cottam，Elisabeth C.Lowe＆James P.R.ConnollyInstitute of Environmental Science and Research，惠灵顿，新西兰Rhys T。

WhiteAustralian Infectious Diseases Research Centre and School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD, AustraliaRhys T. White & Scott A. BeatsonNewcastle University Translation and Clinical Research Institute, Newcastle University, NE2 4HH, Newcastle-upon-Tyne, UKLauren C.

澳大利亚昆士兰大学澳大利亚传染病研究中心和化学与分子生物科学学院，澳大利亚昆士兰布里斯班Arhys T.White＆Scott A.BeatsonNewcastle University Translation and Clinical Research Institute，纽卡斯尔大学，NE2 4HH，纽卡斯尔泰恩河畔纽卡斯尔，UKLauren C。

Beck & Christopher J. StewartDepartment of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, AustraliaRhys GrinterAuthorsCurtis CottamView author publicationsYou can also search for this author in.

贝克和克里斯托弗·斯图尔特（Beck＆ChristopherJ.Stewart）莫纳什大学生物医学发现研究所微生物学系，澳大利亚维多利亚州克莱顿（Clayton），Arhys GrinterauthorsCurtisCottamview作者出版物您也可以在中搜索这位作者。

PubMed Google ScholarRhys T. WhiteView author publicationsYou can also search for this author in

PubMed Google ScholarRhys T.WhiteView作者出版物您也可以在

PubMed Google ScholarLauren C. BeckView author publicationsYou can also search for this author in

PubMed Google ScholarLauren C.BeckView作者出版物您也可以在

PubMed Google ScholarChristopher J. StewartView author publicationsYou can also search for this author in

PubMed Google ScholarChristopher J.StewartView作者出版物您也可以在

PubMed Google ScholarScott A. BeatsonView author publicationsYou can also search for this author in

PubMed Google ScholarScott A.BeatsonView作者出版物您也可以在

PubMed Google ScholarElisabeth C. LoweView author publicationsYou can also search for this author in

PubMed Google ScholarElisabeth C.LoweView作者出版物您也可以在

PubMed Google ScholarRhys GrinterView author publicationsYou can also search for this author in

PubMed Google ScholarRhys GrinterView作者出版物您也可以在

PubMed Google ScholarJames P. R. ConnollyView author publicationsYou can also search for this author in

PubMed谷歌学者James P.R.ConnollyView作者出版物您也可以在

PubMed Google ScholarContributionsC.C. and J.P.R.C conceptualised and designed the research. C.C., R.T.W. and J.P.R.C. performed the research. C.C., R.T.W., L.C.B., R.G. and J.P.R.C. analysed the data. C.J.S., S.A.B., R.G., E.C.L. and J.P.R.C. contributed reagents/analytical tools.

PubMed谷歌学术贡献中心。C、 J.P.R.C对这项研究进行了概念化和设计。C、 C.，R.T.W.和J.P.R.C.进行了这项研究。C、 C.，R.T.W.，L.C.B.，R.G.和J.P.R.C.分析了数据。C、 J.S.，S.A.B.，R.G.，E.C.L.和J.P.R.C.贡献了试剂/分析工具。

C.C. and J.P.R.C. wrote the paper with input from all other authors.Corresponding authorCorrespondence to.

C、 C.和J.P.R.C.在所有其他作者的意见下撰写了这篇论文。对应作者对应。

James P. R. Connolly.Ethics declarations

詹姆斯·P·R·康诺利。道德宣言

Competing interests

相互竞争的利益

The authors declare no competing interests.

作者声明没有利益冲突。

Peer review

同行评审

Peer review information

同行评审信息

Nature Communications thanks David Gally and Gavin Thomas for their contribution to the peer review of this work. A peer review file is available.

Nature Communications感谢David Gally和Gavin Thomas为这项工作的同行评审做出的贡献。同行评审文件可用。

Additional informationPublisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.Supplementary informationSupplementary InformationPeer Review FileDescription of Additional Supplementary FilesSupplementary Data 1Reporting SummarySource dataSource DataRights and permissions.

Additional informationPublisher的注释Springer Nature在已发布地图和机构隶属关系中的管辖权主张方面保持中立。补充信息补充信息同行评审文件其他补充文件的描述补充数据1报告摘要源数据源数据权限。

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.

开放获取本文是根据知识共享署名4.0国际许可证授权的，该许可证允许以任何媒体或格式使用，共享，改编，分发和复制，只要您对原始作者和来源给予适当的信任，提供知识共享许可证的链接，并指出是否进行了更改。

The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.

To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/..

要查看此许可证的副本，请访问http://creativecommons.org/licenses/by/4.0/..

Reprints and permissionsAbout this articleCite this articleCottam, C., White, R.T., Beck, L.C. et al. Metabolism of l-arabinose converges with virulence regulation to promote enteric pathogen fitness.

转载和许可本文引用本文Cottam，C.，White，R.T.，Beck，L.C.等人。L-阿拉伯糖的代谢与毒力调节相融合，以促进肠道病原体的适应性。

Nat Commun 15, 4462 (2024). https://doi.org/10.1038/s41467-024-48933-7Download citationReceived: 18 December 2023Accepted: 16 May 2024Published: 25 May 2024DOI: https://doi.org/10.1038/s41467-024-48933-7Share this articleAnyone you share the following link with will be able to read this content:Get shareable linkSorry, a shareable link is not currently available for this article.Copy to clipboard.

《国家公社》154462（2024）。https://doi.org/10.1038/s41467-024-48933-7Download引文接收日期：2023年12月18日接收日期：2024年5月16日发布日期：2024年5月25日OI：https://doi.org/10.1038/s41467-024-48933-7Share本文与您共享以下链接的任何人都可以阅读此内容：获取可共享链接对不起，本文目前没有可共享的链接。复制到剪贴板。

Provided by the Springer Nature SharedIt content-sharing initiative

由Springer Nature SharedIt内容共享计划提供

CommentsBy submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

评论通过提交评论，您同意遵守我们的条款和社区指南。如果您发现有虐待行为或不符合我们的条款或准则，请将其标记为不合适。

全球产业链接平台

重庆市渝北区金星科技大厦A区5楼512室

联系电话：023-67139735（重庆）

关于我们

产品服务