AbstractBackgroundFusobacterium nucleatum inhabits the oral cavity and affects the progression of gastrointestinal cancer. Our prior findings link F. nucleatum to poor prognosis in oesophageal squamous cell carcinoma via NF-κB pathway. However, its role in oesophagogastric junction and gastric adenocarcinoma remains unexplored.

摘要背景有核梭杆菌存在于口腔中，影响胃肠道癌症的进展。我们先前的发现通过NF-κB途径将F.nucleatum与食管鳞状细胞癌的不良预后联系起来。然而，它在食管胃交界处和胃腺癌中的作用尚未探索。

We investigated whether F. nucleatum influences these cancers, highlighting its potential impact.MethodsTwo cohorts of EGJ and gastric adenocarcinoma patients (438 from Japan, 380 from the USA) were studied. F. nucleatum presence was confirmed by qPCR, FISH, and staining. Patient overall survival (OS) was assessed based on F.

我们调查了F.nucleatum是否影响这些癌症，突出了其潜在的影响。方法研究了两组EGJ和胃腺癌患者（438名来自日本，380名来自美国）。F、 通过qPCR，FISH和染色证实了核的存在。根据F评估患者总生存期（OS）。

nucleatum positivity. EGJ and gastric adenocarcinoma cell lines were exposed to F. nucleatum to study molecular and phenotypic effects, validated in xenograft mouse model.ResultsIn both cohorts, F. nucleatum-positive EGJ or gastric adenocarcinoma patients had notably shorter OS. F. nucleatum positivity decreased in more acidic tumour environments.

核阳性。将EGJ和胃腺癌细胞系暴露于F.nucleatum以研究分子和表型效应，并在异种移植小鼠模型中验证。结果在这两个队列中，F.nucleatum阳性EGJ或胃腺癌患者的OS明显较短。F、 在更酸性的肿瘤环境中，细胞核阳性率降低。

Cancer cell lines with F. nucleatum showed enhanced proliferation and NF-κB activation. The xenograft model indicated increased tumour growth and NF-κB activation in F. nucleatum-treated cells. Interestingly, co-occurrence of F. nucleatum and Helicobacter pylori, a known risk factor, was rare.ConclusionsF.

具有F.nucleatum的癌细胞系显示出增强的增殖和NF-κB活化。异种移植模型表明F.nucleatum处理的细胞中肿瘤生长和NF-κB活化增加。。结论SF。

nucleatum can induce the NF-κB pathway in EGJ and gastric adenocarcinomas, leading to tumour progression and poor prognosis..

细胞核可诱导EGJ和胃腺癌中的NF-κB通路，导致肿瘤进展和预后不良。。

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Fig. 1: Fusobacterium nucleatum (F. nucleatum) exacerbates the prognosis of oesophagogastric junction (EGJ) and gastric cancers.Fig. 2: Number of Fusobacterium nucleatum (F. nucleatum)-positive cases or high F. nucleatum DNA cases and acidic environment in oesophageal cancer, oesophagogastric junction (EGJ) cancer, and gastric cancer.Fig.

图1：核梭杆菌（F.nucleatum）加剧了食管胃交界处（EGJ）和胃癌的预后。图2：食管癌，食管胃连接（EGJ）癌和胃癌中有核梭杆菌（F.nucleatum）阳性病例或高F.nucleatum DNA病例和酸性环境的数量。图。

3: Fusobacterium nucleatum (F. nucleatum) promotes cell proliferation, migration, and invasion in oesophagogastric junction (EGJ) and gastric cancer cell lines.Fig. 4: The NF-κB pathway and in vivo model.Fig. 5: Association of Fusobacterium nucleatum (F. nucleatum) and Helicobacter pylori (H. pylori) in upper gastrointestinal cancer..

3： 。图4:NF-κB途径和体内模型。图5：上消化道癌中有核梭杆菌（F.nucleatum）和幽门螺杆菌（H.pylori）的关联。。

Data availability

数据可用性

The datasets generated and/or analysed during the current study are available from the corresponding author on reasonable request.

在当前研究期间生成和/或分析的数据集可根据合理要求从通讯作者处获得。

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Download referencesAcknowledgementsWe thank J. Iacona, Ph.D., from Edanz (https://jp.edanz.com/ac) for editing this manuscript.FundingThis work was supported in part by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (grant numbers 17H04273, 17K19702, and 17KK0195 to Y.B.).Author informationAuthors and AffiliationsDepartment of Gastroenterological Surgery, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto, 860-8556, JapanYoshihiro Hara, Yoshifumi Baba, Eri Oda, Kazuto Harada, Kohei Yamashita, Tasuku Toihata, Keisuke Kosumi, Masaaki Iwatsuki, Yuji Miyamoto & Hideo BabaDivision of Translational Research and Advanced Treatment Against Gastrointestinal Cancer, Kumamoto University, 1-1-1 Honjo, Chuoku, Kumamoto, 860-8556, JapanYoshifumi BabaDepartment of Gastrointestinal Medical Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USAKohei Yamashita & Jaffer A.

下载参考文献致谢我们感谢Edanz的J.Iacona博士(https://jp.edanz.com/ac)。资助这项工作部分得到了日本科学促进会科学研究资助（Y.B.的资助号17H04273、17K19702和17KK0195）。作者信息作者和附属机构熊本大学医学研究生院胃肠外科，1-1-1 Honjo，Chuo ku，Kumamoto，860-8556，JapanYoshihiro Hara，Yoshifumi Baba，Eri Oda，Kazuto Harada，Kohei Yamashita，Tasuku Toihata，Keisuke Kosumi，Masaaki Iwatsuki，Yuji Miyamoto＆Hideo BabaDivision转化研究和胃肠道癌症的高级治疗，熊本大学，1-1-1 Honjo，Chuoku，Kumamoto，860-8556，JapanYoshifumi BabaDepartment of Gintestinal Medical Oncology，德克萨斯大学MD安德森癌症中心，德克萨斯州休斯顿，USAKohei Yamashita＆Jaffer A。

AjaniDepartment of Microbiology, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto, 860-8556, JapanHiroyasu Tsutsuki & Tomohiro SawaDepartment of Pathology, UT M. D. Anderson Cancer Center, Houston, USAQiong Gan & Rebecca E. WatersDepartment of Cell Pathology, Graduate School of Medical Sciences, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto, 860-8556, JapanYoshihiro KomoharaAuthorsYoshihiro HaraView author publicationsYou can also search for this author in.

熊本大学医学研究生院微生物学系，熊本中央区本州1-1-1，860-8556，日本海罗亚苏·津津斯基和智弘·萨瓦病理学系，UT安德森癌症中心，休斯顿，USAQiong Gan＆Rebecca E.WatersDepartment of Cell Pathology，Graduate School of Medical Sciences，Faculty of Life Sciences，Kumamoto，860-8556，JapanYoshihiro KomoharaAuthorYoshihiro HaraView author Publications你也可以搜索这个作者。

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PubMed Google ScholarContributionsYH conducted experiments and contributed to manuscript writing. YB provided guidance and supervision throughout the project. EO, KH, KY, TT, KK, MI, YM, and QG, REW were involved in data collection and analysis. HT and TS provided guidance on microbial experimental techniques.

PubMed Google ScholarContributionsYH进行了实验并为稿件撰写做出了贡献。YB在整个项目中提供指导和监督。EO，KH，KY，TT，KK，MI，YM和QG，REW参与了数据收集和分析。HT和TS为微生物实验技术提供了指导。

YK assisted in the confirmation of pathological specimens. JAA and HB supervised the project. All authors contributed to the conception, design, and interpretation of the study, and critically reviewed and approved the final manuscript.Corresponding authorsCorrespondence to.

YK协助确认病理标本。JAA和HB监督了该项目。所有作者都为研究的概念，设计和解释做出了贡献，并批判性地审查和批准了最终手稿。通讯作者通讯。

Yoshifumi Baba or Hideo Baba.Ethics declarations

吉文芭芭或秀雄芭芭。道德宣言

Competing interests

相互竞争的利益

The authors declare no competing interests.

作者声明没有利益冲突。

Ethics approval and consent to participate

道德批准和同意参与

Informed consent was obtained from all the participants. This study was conducted in accordance with the guidelines of the Declaration of Helsinki and approved by the Institutional Review Board of Kumamoto University (#1365).

获得了所有参与者的知情同意。这项研究是根据赫尔辛基宣言的指导方针进行的，并得到熊本大学机构审查委员会的批准（#1365）。

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Additional informationPublisher的注释Springer Nature在已发布的地图和机构隶属关系中的管辖权主张方面保持中立。补充信息补充材料补充数字权利和许可Pringer Nature或其许可人（例如协会或其他合作伙伴）根据与作者或其他权利持有人的出版协议对本文拥有专有权；本文接受稿件版本的作者自行存档仅受此类出版协议和适用法律的条款管辖。转载和许可本文引用本文Hara，Y.，Baba，Y.，Oda，E。

et al. Presence of Fusobacterium nucleatum in relation to patient survival and an acidic environment in oesophagogastric junction and gastric cancers..

等。有核梭杆菌的存在与患者生存以及食管胃交界处和胃癌中的酸性环境有关。。

Br J Cancer (2024). https://doi.org/10.1038/s41416-024-02753-0Download citationReceived: 25 February 2024Revised: 24 May 2024Accepted: 05 June 2024Published: 12 July 2024DOI: https://doi.org/10.1038/s41416-024-02753-0Share this articleAnyone you share the following link with will be able to read this content:Get shareable linkSorry, a shareable link is not currently available for this article.Copy to clipboard.

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