We developed click editors, comprising HUH endonucleases, DNA-dependent DNA polymerases and CRISPR–Cas9 nickases, which together enable programmable precision genome engineering from simple DNA templates.

我们开发了click编辑器，包括HUH核酸内切酶，DNA依赖性DNA聚合酶和CRISPR-Cas9切口酶，它们共同实现了从简单DNA模板进行可编程精确基因组工程。

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Access Nature and 54 other Nature Portfolio journalsGet Nature+, our best-value online-access subscription24,99 € / 30 dayscancel any timeLearn moreSubscription info for Chinese customersWe have a dedicated website for our Chinese customers. Please go to naturechina.com to subscribe to this journal.Go to naturechina.comBuy this articlePurchase on Springer LinkInstant access to full article PDFBuy nowPrices may be subject to local taxes which are calculated during checkout.

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Fig. 1: Click editing enzymes and mechanism.

图1：点击编辑酶和机制。

ReferencesHalperin, S. O. et al. CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window. Nature 550, 248–252 (2018). This manuscript describes the fusion of an error-prone polymerase to CRISPR–Cas9 to mutagenize specified genomic regions.Article

参考文献Halperin，S.O。等人。CRISPR引导的DNA聚合酶能够在可调窗口中使所有核苷酸多样化。自然550248-252（2018）。该手稿描述了易错聚合酶与CRISPR-Cas9的融合，以诱变特定的基因组区域。文章

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Anzalone，A.V.等人在没有双链断裂或供体DNA的情况下搜索和替换基因组编辑。自然576149-157（2019）。本文报道了prime编辑的发展，这是一种精确的基因组编辑方法，使用逆转录酶将特定编辑写入基因组。文章。

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Download referencesAdditional informationPublisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.This is a summary of: Ferreira da Silva, J. et al. Click editing enables programmable genome writing using DNA polymerases and HUH endonucleases.

下载参考其他信息出版商的注释Springer Nature对于已发布地图和机构隶属关系中的管辖权主张保持中立。这是一个总结：Ferreira da Silva，J。等人。点击编辑可以使用DNA聚合酶和HUH核酸内切酶进行可编程基因组写入。

Nat. Biotechnol. https://doi.org/10.1038/s41587-024-02324-x (2024).Rights and permissionsReprints and permissionsAbout this articleCite this article Precise and versatile genome editing with click editors..

美国国家生物技术公司。https://doi.org/10.1038/s41587-024-02324-x（2024年）。权利和许可打印和许可关于本文引用本文使用click Editor进行精确而通用的基因组编辑。。

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