BUSAN, South Korea--(BUSINESS WIRE)--At the 22nd Human Proteome Organization World Congress 2023, Bruker Corporation (Nasdaq: BRKR) today announced advances in instrumental methods, consumables and software for 4D-Proteomics, MALDI HiPLEX-IHC spatial tissue proteomics, 4D-Lipidomics and 4D-Metabolomics..

韩国釜山-（商业线）-在第22届人类蛋白质组组织2023年世界大会上，布鲁克公司（纳斯达克股票代码：BRKR）今天宣布了4D蛋白质组学，MALDI HiPLEX IHC空间的仪器方法，耗材和软件方面的进展组织蛋白质组学，4D脂质组学和4D代谢组学。。

A. Novel scalable AI-powered TIMSquant™ for label-free quantification

A、 新颖的可扩展AI供电TIMSquant™ 无标签量化

Bruker introduces TIMSquant a scalable, CCS-enabled AI-powered label-free quantification algorithm integrated into Bruker ProteoScape™. TIMSquant performs accurate quantification of peptides and proteins present in 100s or 1000s samples by using global retention time and collision cross-section (CCS) prediction models.

布鲁克介绍了TIMSquant一个可扩展的，支持CCS的AI支持的无标记量化算法，集成到布鲁克蛋白质景观中™. TIMSquant通过使用全局保留时间和碰撞横截面（CCS）预测模型对100s或1000s样品中存在的肽和蛋白质进行准确定量。

Accurate, large-scale CCS data further increases specificity and quantification consistency based on previously identified peptides and aligned missing values..

准确的大规模CCS数据进一步提高了基于先前鉴定的肽和比对缺失值的特异性和定量一致性。。

Bruker ProteoScape is Bruker’s comprehensive GPU-powered, TIMS DIA-NN-based proteomics software optimized for CCS-centric informatics, and it now comes with the BPS Novor module for de novo peptide sequencing for immunopeptidomics. BPS Novor was co-developed with Rapid Novor Inc., a Canadian software and CRO company.

Bruker ProteoScape是Bruker全面的GPU功能，基于TIMS DIA NN的蛋白质组学软件，针对以CCS为中心的信息学进行了优化，现在它配备了BPS Novor模块，用于免疫肽组学的从头肽测序。BPS Novor与加拿大软件和CRO公司Rapid Novor Inc.共同开发。

TIMS DIA-NN is a customized version of highly regarded DIA-NN software by Demichev and collaborators..

TIMS DIA-NN是Demichev及其合作者高度重视的DIA-NN软件的定制版本。。

Dr. Kosuke Ogata, Assistant Professor at Kyoto University, commented: 'Ultra-high speed LC-MS from large-scale clinical cohorts harnessing the high duty-cycle of timsTOF technology is a focus of our laboratory. Analysis of non-proteotypic peptides is critical to our research. We have installed the BPS Novor de novo software on Bruker ProteoScape (formerly PaSER) and we are impressed with the real-time speed and high-quality de novo sequences produced.'.

京都大学助理教授Kosuke Ogata博士评论说：“利用timsTOF技术的高工作周期的大规模临床队列的超高速LC-MS是我们实验室的重点。非蛋白型肽的分析对我们的研究至关重要。我们在Bruker ProteoScape（前身为PaSER）上安装了BPS Novor de novo软件，我们对生成的实时速度和高质量的de novo序列印象深刻。

Dr. Hanno Steen, Associate Professor at Harvard Medical School & Director of Proteomics at Boston Children's Hospital, stated: “We focus on the discovery and validation of biomarkers from body fluids, such as plasma, serum, CSF, saliva and urine in high throughput. We leverage the robustness of our timsTOF instruments and real-time Bruker ProteoScape to build large spectral libraries and analyze 1000s of clinical research samples.

哈佛医学院副教授，波士顿儿童医院蛋白质组学主任Hanno Steen博士，他说：“我们专注于高通量发现和验证体液中的生物标志物，如血浆，血清，脑脊液，唾液和尿液。我们利用timsTOF仪器和实时Bruker ProteoScape的强大功能来构建大型光谱库并分析1000个临床研究样本。

We have recently expanded our bioinformatics pipeline to run on ProteoScape, and are truly excited by the realistic potential to apply this for the analysis of 10,000s of LC/MS runs in real time to decipher the pathology of pediatric, immunologic, and neurological diseases.”.

我们最近扩展了我们的生物信息学管道，以便在ProteoScape上运行，并且真正激发了将其应用于实时分析10000s LC/MS运行以破译儿科，免疫和神经疾病病理学的现实潜力“。

B. Novel dia-PASEF scan modes

B、 新颖的dia PASEF扫描模式

Bruker offers selected upgrades to timsControl acquisition software with the key advances of 300 Hz PASEF and VistaScan™ technology as downloads from the Bruker web page (Reference 3). The 300Hz dda-PASEF acquisition now allows up to 18,000 CCS-enabled MS/MS spectra per minute with very short LC gradients.

随着300hz PASEF和VistaScan的关键进步，布鲁克提供了timsControl采集软件的选定升级™ 技术从Bruker网页下载（参考文献3）。300Hz dda PASEF采集现在允许每分钟多达18000个CCS启用的MS/MS谱图，并具有非常短的LC梯度。

VistaScan allows scanning the quadrupole synchronously with the TIMS ramp during the mobility scan to optimally cover the ion population in the ion-mobility-mass to charge plane. (References 1 & 2)..

VistaScan允许在迁移率扫描期间与TIMS斜坡同步扫描四极杆，以最佳地覆盖离子迁移率质荷比平面中的离子群。（参考文献1和2）。。

VistaScan enables midia-PASEF, exclusively licensed from Johannes Gutenberg University Mainz. Bruker is initiating a limited early-access program for midia-PASEF for immunopeptidomics and post-translational modifications (PTMs) on the ultra-sensitive timsTOF Ultra. These applications benefit from the sensitivity-with-speed of midia-PASEF, as well as the DDA-like precursor traceability that can be extracted from midia-PASEF data..

VistaScan支持midia PASEF，该产品仅由Johannes Gutenberg University Mainz授权。布鲁克正在为midia PASEF启动一个有限的早期访问程序，用于超敏感timsTOF ultra的免疫肽组学和翻译后修饰（PTM）。这些应用受益于midia PASEF速度的灵敏度，以及可从midia PASEF数据中提取的类似DDA的前体可追溯性。。

C. PreOmics ENRICH Kit for affordable, high-throughput ~3,500 PG plasma proteomics

C、 PreOmics ENRICH试剂盒，可负担得起的高通量~3500 PG血浆蛋白质组学

Bruker, together with PreOmics and Biognosys are now offering further improved high-throughput, affordable, medium-depth plasma proteomics kits and CRO services for reliably quantifying ~3,500 protein groups using PreOmics ENRICH-iST microbeads (SP3 technology) from minimal sample amounts and various species without antibodies/aptamers..

布鲁克与PreOmics和Biognosys一起提供进一步改进的高通量，价格合理的中等深度血浆蛋白质组学试剂盒和CRO服务，使用PreOmics ENRICH iST microbeads（SP3技术）从最少的样品量和各种样品中可靠地定量~3500个蛋白质组没有抗体/适体的物种。。

D. PepSep™ ULTRA XL and PepSep™ MAX HT columns

D、 胃蛋白酶™ ULTRA XL和PepSep™ 最大HT列

Bruker announced the launch of the PepSep™ ULTRA XL and PepSep™ MAX HT columns. The PepSep ULTRA XL was designed for long LC gradients, e.g. for DDA-PASEF spectral library generation, which can improve confidence and coverage in peptide and protein identification during short gradients in DIA-PASEF analysis.

布鲁克宣布推出PepSep™ ULTRA XL和PepSep™ 最大HT列。PepSep ULTRA XL专为长LC梯度设计，例如用于DDA-PASEF光谱库生成，可提高DIA-PASEF分析中短梯度期间肽和蛋白质鉴定的置信度和覆盖率。

The PepSep MAX HT is optimized for short-gradients (<15 min run0times) used for high throughput proteomics..

PepSep MAX HT针对用于高通量蛋白质组学的短梯度（<15分钟运行时间）进行了优化。。

Dr. Byoung-Kyu Cho, Assistant Professor at Washington University School of Medicine and Proteomics Lead at Mass Spectrometry Technology Access Center (MTAC) commented: “PepSep MAX HT columns have brought a remarkable transformation to our mass spectrometry endeavors. This compact column stands out with superior throughput, enduring robustness, and an interface that is user-friendly, making it easy to operate.

Byoung-Kyu Cho博士，华盛顿大学医学与蛋白质组学学院质谱技术访问中心（MTAC）负责人助理教授评论说：“PepSep MAX HT色谱柱为我们的质谱研究带来了显着的转变。这种紧凑色谱柱具有卓越的通量，持久的稳健性，以及用户友好的界面，使其易于操作。

To all colleagues in search of top-tier high-throughput data, I endorse the PepSep MAX HT.”.

对于所有寻找顶级高通量数据的同事，我认可PepSep MAX HT“。

E. TwinScape digital twin functionality in Bruker ProteoScape software

To ensure optimal run conditions for every sample, Bruker introduces TwinScape™, a digital twin for the timsTOF platform, using Bruker ProteoScape software and the Biognosys iRT kit. TwinScape continuously monitors key parameters to ensure that complex assays and large studies are run under optimal conditions, supporting real-time QC with the iRT kit, which contains eleven non-naturally occurring synthetic peptides.

为了确保每个样品的最佳运行条件，布鲁克引入了TwinScape™, 使用Bruker ProteoScape软件和Biognosys iRT套件为timsTOF平台提供数字双胞胎。TwinScape持续监控关键参数，以确保在最佳条件下运行复杂的测定和大型研究，并使用iRT试剂盒支持实时QC，该试剂盒包含11种非天然合成肽。

The iRT kit peptides have been optimized for stability, sensitivity, rt spacing and peptide CCS..

iRT试剂盒肽已针对稳定性，灵敏度，rt间隔和肽CCS进行了优化。。

Dr. Roman Fischer, Associate Professor at University of Oxford, commented: “At the Centre for Translational Proteomics (CTP), we provide services for clients interested in deep proteomes from small sample amounts. Clinical proteomics and spatial proteomics both of require characterization of large sample cohorts at high throughput.

牛津大学副教授Roman Fischer博士评论说：“在转化蛋白质组学中心（CTP），我们为小样本量的深度蛋白质组感兴趣的客户提供服务。临床蛋白质组学和空间蛋白质组学都需要表征高通量的大样本队列。

Bruker ProteoScape monitors the sample and data quality in real-time, exploiting the outstanding robustness of the timsTOF platforms for high-throughput deep proteomics.”.

布鲁克ProteoScape实时监控样品和数据质量，利用timsTOF平台对高通量深度蛋白质组学的出色稳健性“。

F. Biological knowledge generation from 4D-Proteomics with Mass Dynamics

F、 具有质量动力学的4D蛋白质组学产生生物学知识

Bruker’s collaboration with Prof. Andrew Webb at WEHI, co-founder of Mass Dynamics Pty Ltd, has led to the integration of data from Bruker ProteoScape™ and now Spectronaut® with Mass Dynamics interactive visualization tools for knowledge extraction to allow scientists to answer more biological questions..

布鲁克与Mass Dynamics Pty Ltd联合创始人WEHI的Andrew Webb教授的合作促成了布鲁克ProteoScape数据的整合™ 现在Spectronaut®具有用于知识提取的质量动力学交互式可视化工具，可让科学家回答更多生物学问题。。

G. Novel SpatialOMx workflows enhancing 4D-Proteomics

G、 新型SpatialOMx工作流程增强4D蛋白质组学

In collaboration with AmberGen, Bruker further advances the novel MALDI HiPLEX-IHC workflow for spatial proteomics based on antibody-based localization of target proteins with a significantly enhanced probe selection and robust workflows similar to IHC protocols, adapting HiPLEX-IHC to a variety of areas from immunooncology to neuropathology..

Bruker与AmberGen合作，进一步推进了基于抗体的靶蛋白定位的空间蛋白质组学新型MALDI-HiPLEX IHC工作流程，具有显着增强的探针选择和类似于IHC协议的强大工作流程，使HiPLEX IHC适用于各种领域从免疫组学到神经病理学。。

Dr. Bernd Bodenmiller, Professor University of Zuerich and ETH Zuerich, and Director of the Department of Quantitative Biomedicine, noted: “Recent research shows that spatial proteomics provides biological context about tissue neighborhoods by providing spatially defined biomarkers. The cellular arrangements and location of proteins in the tissue microenvironment elucidates biological mechanisms in disease or homeostasis, supporting novel precision medicine applications.

Bernd Bodenmiller博士，Zuerich大学教授和ETH Zuerich教授，定量生物医学系主任，指出：“最近的研究表明，空间蛋白质组学通过提供空间定义的生物标志物提供了有关组织邻域的生物学背景。组织微环境中蛋白质的细胞排列和位置阐明了疾病或体内平衡的生物学机制，支持新型精密医学应用。

Using HiPLEX-IHC tissue imaging on our timsTOF fleX enhances our multiomic spatial view of tissue ecosystems.”.

在我们的timsTOF fleX上使用HiPLEX IHC组织成像增强了我们对组织生态系统的多组学空间视图“。

H. Novel MetaboScape software for 4D multiomic research

H、 用于4D多组学研究的新型MetaboScape软件

In the new release of MetaboScape®, CCS-enabled 4D-Lipidomics is complemented by integrating semi-quantitative analysis utilizing stable isotopically labeled lipid standards.

在MetaboScape®的新版本中，CCS启用的4D脂质组学通过利用稳定的同位素标记脂质标准整合半定量分析得到补充。

Dr. Heiko Hayen, Institute of Inorganic and Analytical Chemistry, University of Münster, Germany, said: “The new CCS-enabled workflow with T-ReX 4D in MetaboScape allows us to accurately normalize our lipid class concentrations with labelled internal standards. By adopting this solution, we observed a noticeable improvement in standard deviations among covered lipids, enhancing reproducibility in our lipidomics research.”.

德国明斯特大学无机与分析化学研究所Heiko Hayen博士，他说：“在MetaboScape中使用T-ReX 4D的新CCS启用工作流程使我们能够使用标记的内标准确标准化我们的脂质类别浓度。通过采用此解决方案，我们观察到覆盖脂质的标准偏差显着改善，提高了重复性我们的脂质组学研究“。

Dr. Viswa Colluru, CEO and Founder of Enveda Biosciences, said: “The open software ecosystem provided by Bruker is at the foundation of our discovery platform. Their CCS-enabled workflows give us an edge in discerning compounds from nature with better resolution. Bruker’s commitment to innovation and customer support makes them a great partner and helps achieve our mission of translating nature into medicine.'.

Enveda Biosciences的首席执行官兼创始人Viswa Colluru博士，他说：“布鲁克提供的开放软件生态系统是我们发现平台的基础，他们的CCS工作流程使我们在以更好的分辨率辨别自然界中的化合物方面具有优势。布鲁克对创新和客户支持的承诺使他们成为一个伟大的合作伙伴，并帮助实现我们将自然转化为医学的使命。”。

References:

参考文献：

Distler et al., bioRxiv 2023.01.30.526204, https://doi.org/10.1101/2023.01.30.526204

Distler等人，bioRxiv 2023.01.30.526204，https://doi.org/10.1101/2023.01.30.526204

Skowronek et al., Molecular & Cellular Proteomics, Feb 2023; 22(2):100489-100489, https://doi.org/10.1016/j.mcpro.2022.100489

Skowronek等，分子与细胞蛋白质组学，2023年2月；22(2):100489-100489, https://doi.org/10.1016/j.mcpro.2022.100489

https://www.bruker.com/protected/de/services/software-downloads/mass-spectrometry/acquisition-software.html

https://www.bruker.com/protected/de/services/software-downloads/mass-spectrometry/acquisition-software.html

About Bruker Corporation (Nasdaq: BRKR)

关于布鲁克公司（纳斯达克股票代码：BRKR）

Bruker is enabling scientists to make breakthrough discoveries and develop new applications that improve the quality of human life. Bruker’s high performance scientific instruments and high value analytical and diagnostic solutions enable scientists to explore life and materials at molecular, cellular, and microscopic levels.

布鲁克正在使科学家们能够取得突破性的发现并开发能够提高人类生活质量的新应用。布鲁克的高性能科学仪器和高价值的分析和诊断解决方案使科学家能够在分子，细胞和微观层面探索生命和材料。

In close cooperation with our customers, Bruker is enabling innovation, improved productivity, and customer success in life science molecular and cell biology research, in applied and pharma applications, in microscopy and nanoanalysis, as well as in industrial applications. Bruker offers differentiated, high-value life science and diagnostics systems and solutions in preclinical imaging, clinical phenomics research, proteomics and multiomics, spatial and single-cell biology, functional structural and condensate biology, as well as in clinical microbiology and molecular diagnostics.

与客户密切合作，布鲁克正在生命科学分子和细胞生物学研究，应用和制药应用，显微镜和纳米分析以及工业应用方面实现创新，提高生产率和客户成功。布鲁克公司在临床前成像，临床表型研究，蛋白质组学和多组学，空间和单细胞生物学，功能结构和凝聚生物学以及临床微生物学和分子诊断学方面提供差异化，高价值的生命科学和诊断系统和解决方案。

Please visit www.bruker.com..

请访问www.bruker.com。。