基于基因组挖掘和HSQC-TOCSY从黄霉菌链霉菌TRM90047中发现抗结核分枝杆菌沙漠霉素-动脉网

Discovery of anti-Mycobacterium tuberculosis desertomycins from Streptomyces flavofungini TRM90047 based on genome mining and HSQC-TOCSY

Nature 等信源发布 2024-07-24 12:32

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AbstractTuberculosis caused by Mycobacterium tuberculosis (M. tb) is a major public health problem with high morbidity and mortality worldwide. In our previous study, we found that a fermentation product of Streptomyces flavofungini TRM90047 exhibited anti-M. tb activity and decreased the expression level of several genes, including rpsL, Rplc and ClpC1.

摘要由结核分枝杆菌（M.tb）引起的结核病是全球高发病率和高死亡率的主要公共卫生问题。在我们之前的研究中，我们发现黄蕈链霉菌TRM90047的发酵产物表现出抗结核分枝杆菌活性，并降低了几个基因的表达水平，包括rpsL，Rplc和ClpC1。

Guided by heteronuclear single quantum correlation-total correlation spectroscopy (HSQC-TOCSY) fingerprints and genome mining, we isolated two new 44-membered macrolides, desertomycin 44-1 (1) and desertomycin 44-2 (2), together with known desertomycin A (3) from S. flavofungini TRM90047. Three desertomycins showed anti-M.

在异核单量子相关全相关光谱（HSQC-TOCSY）指纹图谱和基因组挖掘的指导下，我们分离了两种新的44元大环内酯类，沙漠霉素44-1（1）和沙漠霉素44-2（2），以及已知的沙漠霉素A（3）来自S.flavofungini TRM90047。三种沙漠霉素显示出抗M。

tb activity. The EC50 values of desertomycin A, desertomycin 44-1 and desertomycin 44-2 were 25 µg/mL, 25 µg/mL and 50 µg/mL, respectively. Molecular docking analyses revealed that the isolated desertomycins bound well to the RPSL, RPLC and CLPC1 proteins. In the present study, we describe the discovery of new anti-M.

结核病活动。沙漠霉素A，沙漠霉素44-1和沙漠霉素44-2的EC50值分别为25µg/mL，25µg/mL和50µg/mL。分子对接分析表明，分离的沙漠霉素与RPSL，RPLC和CLPC1蛋白结合良好。在本研究中，我们描述了新的抗M的发现。

tb compounds guided by genome mining, HSQC-TOCSY and anti-M. tb bioassays..

由基因组挖掘，HSQC-TOCSY和抗结核分枝杆菌生物测定指导的结核化合物。。

IntroductionTuberculosis (TB) is a major public health problem worldwide caused by Mycobacterium tuberculosis (M. tb)1. Long-term drug use has led to the emergence of multidrug-resistant (MDR) M. tb and extensively drug-resistant (XDR) M. tb, increasing the difficulty of controlling TB2. Approximately 70% of available antimicrobial agents are derived from microorganisms.

引言结核病（TB）是由结核分枝杆菌（M.TB）1引起的全球主要公共卫生问题。长期使用药物导致耐多药（MDR）结核分枝杆菌和广泛耐药（XDR）结核分枝杆菌的出现，增加了控制TB2的难度。大约70%的可用抗菌剂来自微生物。

It is well known that natural products and some of their derivatives exhibit better inhibitory activity against M. tb3,4,5. Streptomyces is among the most important sources of bioactive antimicrobial metabolites. Streptomycin is a well-known anti-M. tb compound isolated from Streptomyces griseus that targets protein synthesis pathways involving the rpsL gene of M.

众所周知，天然产物及其一些衍生物对M.tb3,4,5具有更好的抑制活性。链霉菌是生物活性抗菌代谢物的最重要来源之一。链霉素是从灰链霉菌中分离出的一种众所周知的抗结核分枝杆菌化合物，其靶向涉及M的rpsL基因的蛋白质合成途径。

tb6. Streptomyces D22-7B was determined to be a producer of cyclomarin A, which was previously identified from a marine origin and binds specifically to the ClpC1 N-terminal domain of M. tb7. Kanamycin, a compound produced by Streptomyces kanamyceticus ATCC 12,853, is also used as a second-line drug to treat TB8.

tb6。链霉菌D22-7B被确定为香豆素a的产生者，香豆素a先前是从海洋来源鉴定的，并与M.tb7的ClpC1 N末端结构域特异性结合。卡那霉素是由卡那霉素链霉菌ATCC 12853产生的化合物，也被用作治疗TB8的二线药物。

Desertomycins9 are a series of macrolides with a 42/44-membered ring and are synthesized by polyketide synthase (PKS), desertomycins contain side chains with various components, including carboxyl, amino, guanidinium, hemiacetal and butyrolactone,, and various glycosides/aglycones, leading to structural diversity.

沙漠霉素9是一系列具有42/44元环的大环内酯类化合物，由聚酮合酶（PKS）合成，沙漠霉素含有具有各种成分的侧链，包括羧基，氨基，胍基，半缩醛和丁内酯，以及各种糖苷/糖苷配基，导致结构多样性。

Desertomycins with 42-membered rings have been discovered, including desertomycin A-X10,11,12,13,14,15, desertomycins with 44-membered rings include oasomycin A-E16, among which desertomycin G has shown anti-M. tb activity with a minimal inhibitory concentration (MIC) of 16 µg/mL14.Genome mining is widely used to explore natural products of actinomycetes because researchers can predict biosynthesis gene clusters of secondary.

已经发现了具有42元环的沙漠霉素，包括沙漠霉素A-X10,11,12,13,14,15，具有44元环的沙漠霉素包括oasomycin A-E16，其中沙漠霉素G具有抗结核分枝杆菌活性，最小抑制浓度（MIC）为16µG/mL14。基因组挖掘被广泛用于探索放线菌的天然产物，因为研究人员可以预测次级的生物合成基因簇。

Data availability

数据可用性

All data generated or analysed during this study are included in this published article (and its Supplementary Information files).

本研究期间生成或分析的所有数据均包含在本文（及其补充信息文件）中。

ReferencesBagcchi, S. WHO’s Global Tuberculosis Report 2022. Lancet Microbe 4, e20 (2023).Article

参考Bagcchi，S。WHO《2022年全球结核病报告》。柳叶刀微生物4，e20（2023）。文章

PubMed

Google Scholar

谷歌学者

Huynh, J. & Marais, B. J. Multidrug-resistant tuberculosis infection and disease in children: A review of new and repurposed drugs. Ther. Adv. Infect. Dis. 6, 2049936119864737 (2019).PubMed

Huynh，J。＆Marais，B.J。儿童耐多药结核病感染和疾病：新药和再利用药物的综述。他们。Adv.感染。Dis。62049936119864737（2019）。PubMed出版社

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Luo, X. et al. Antituberculosis compounds from a deep-sea-derived fungus Aspergillus sp. SCSIO Ind09F01. Nat. Prod. Res. 31, 1958–1962 (2017).Article

。《国家生产法》第31号决议，1958年至1962年（2017年）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Igarashi, M., Ishizaki, Y. & Takahashi, Y. New antituberculous drugs derived from natural products: current perspectives and issues in antituberculous drug development. J. Antibiot. 71, 15–25 (2017).Article

Igarashi，M.，Ishizaki，Y。＆Takahashi，Y。天然产物衍生的新型抗结核药物：抗结核药物开发的当前观点和问题。J、抗生素。71,15-25（2017）。文章

Google Scholar

谷歌学者

Elad, N. et al. Structure of Type-I Mycobacterium tuberculosis fatty acid synthase at 3.3 Å resolution. Nat. Commun. 9, 3886 (2018).Article

Elad，N。等人。I型结核分枝杆菌脂肪酸合酶的结构，分辨率为3.3Å。国家公社。93886（2018）。文章

ADS

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Mishra, S. K. et al. Drug development against tuberculosis: Impact of alkaloids. Eur. J. Med. Chem. 137, 504–544 (2017).Article

Mishra，S.K.等人，《抗结核药物开发：生物碱的影响》。欧洲医学化学杂志。137504-544（2017）。文章

ADS

CAS

中科院

PubMed

Google Scholar

谷歌学者

Waters, B. et al. Identifying protein kinase inhibitors using an assay based on inhibition of aerial hyphae formation in Streptomyces. J. Antibiot. (Tokyo) 55, 407–416 (2002).Article

Waters，B.等人使用基于抑制链霉菌空中菌丝形成的测定法鉴定蛋白激酶抑制剂。J、抗生素。（东京）55407-416（2002）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Alangaden, G. J. et al. Mechanism of resistance to amikacin and kanamycin in Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 42, 1295–1297 (1998).Article

Alangaden，G.J.等人。结核分枝杆菌对阿米卡星和卡那霉素的耐药机制。抗菌剂。化学试剂。421295-1297（1998）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Bax, A., Aszalos, A., Dinya, Z. & Sudo, K. Structure elucidation of the antibiotic desertomycin through the use of new two-dimensional NMR techniques. J. Am. Chem. Soc 108, 8056–8063 (1986).Article

Bax，A.，Aszalos，A.，Dinya，Z。＆Sudo，K。通过使用新的二维NMR技术对抗生素沙漠霉素进行结构阐明。J、美国化学。Soc 1088056–8063（1986）。文章

CAS

中科院

Google Scholar

谷歌学者

Uri, J., Bognar, R., Bekesi, I. & Varga, B. Desertomycin, a new crystalline antibiotic with antibacterial and cytostatic action. Nature 182, 401 (1958).Article

Uri，J.，Bognar，R.，Bekesi，I。＆Varga，B。沙漠霉素，一种具有抗菌和细胞抑制作用的新型结晶抗生素。自然182401（1958）。文章

ADS

CAS

中科院

PubMed

Google Scholar

谷歌学者

Ivanova, V. New macrolactone of the desertomycin family from Streptomyces spectabilis. Prep. Biochem. Biotechnol. 27, 19–38 (1997).Article

Ivanova，V。来自Streptomyces spectabilis的沙漠霉素家族的新大环内酯。生物化学制剂。生物技术。27，19-38（1997）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Dinya, Z., Sztaricskai, F., Horváth, E., Schaág, J. B. & Varró, K. Studies of the components of crude desertomycin complex by means of electrospray and matrix-assisted laser desorption/ionization mass spectrometric techniques. Rapid Commun. Mass Spectrom. 10, 1439–1448 (2015).Article .

Dinya，Z.，Sztaricskai，F.，Horváth，E.，Schaág，J.B。＆Varró，K。通过电喷雾和基质辅助激光解吸/电离质谱技术研究粗沙漠霉素复合物的成分。快速通讯。质谱。。文章。

ADS

Google Scholar

谷歌学者

Mohamed, O. G. et al. Identification of a new antimicrobial, Desertomycin H, utilizing a modified crowded plate technique. Mar. Drugs 19, 424 (2021).Article

Mohamed，O.G.等人利用改进的拥挤平板技术鉴定了一种新的抗菌剂沙漠霉素H。3月药物19424（2021）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Braña, A. F. et al. Desertomycin G, a new antibiotic with activity against Mycobacterium tuberculosis and human breast tumor cell lines produced by Streptomyces althioticus MSM3, Isolated from the Cantabrian Sea Intertidal macroalgae Ulva sp.. Mar. Drugs 17, 114 (2019).Article

Braña，a.F.等人。沙漠霉素G，一种新的抗生素，具有抗结核分枝杆菌和人类乳腺肿瘤细胞系的活性，由链霉菌（Streptomyces althioticus）MSM3产生，分离自坎塔布海潮间带大型藻类Ulva sp。，Mar.Drugs 17114（2019）。文章

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Schneider, O. et al. N-Succinyltransferase encoded by a cryptic siderophore biosynthesis gene cluster in Streptomyces modifies structurally distinct antibiotics. mBio 13, e0178922 (2022).Article

由链霉菌中隐秘的铁载体生物合成基因簇编码的N-琥珀酰转移酶修饰结构不同的抗生素。mBio 13，e0178922（2022）。文章

PubMed

Google Scholar

谷歌学者

Grabley, S. Oasomycins, new macrolactones of the desertomycin family. Liebigs Ann. Chem. 1993, 573–579 (1993).Article

Grabley，S.Oasomycins，沙漠霉素家族的新大环内酯。Liebigs Ann。化学。1993年，573-579（1993）。文章

Google Scholar

谷歌学者

Bhushan, A., Egli, P. J., Peters, E. E., Freeman, M. F. & Piel, J. Genome mining- and synthetic biology-enabled production of hypermodified peptides. Nat. Chem. 11, 931–939 (2019).Article

Bhushan，A.，Egli，P.J.，Peters，E.E.，Freeman，M.F。＆Piel，J。基因组挖掘和合成生物学使超修饰肽的生产成为可能。自然化学。11931-939（2019）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Buedenbender, L. et al. HSQC-TOCSY fingerprinting-directed discovery of antiplasmodial polyketides from the marine ascidian-derived Streptomyces sp. (USC-16018). Mar. Drugs 16, 189 (2018).Article

Buedenbender，L。等人。HSQC-TOCSY指纹图谱指导从海洋海鞘衍生的链霉菌属（USC-16018）中发现抗疟原虫聚酮化合物。3月药物16189（2018）。文章

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Huang, H. N. et al. rpsL gene analysis associated with Streptomycin resistance in Mycobacterium tuberculosis. Yi chuan xue bao = Acta genetica Sinica 30, 376–381 (2003).Beckert, P. et al. rplC T460C identified as a dominant mutation in linezolid-resistant Mycobacterium tuberculosis strains.

Huang，H.N.等人。与结核分枝杆菌链霉素耐药性相关的rpsL基因分析。易传学报=遗传学报30376-381（2003）。Beckert，P。等人，rplC T460C被鉴定为耐利奈唑胺结核分枝杆菌菌株的显性突变。

Antimicrob. Agents Chemother. 56, 2743–2745 (2012).Article .

抗菌剂。化学试剂。562743-2745（2012）。文章。

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Kar, N. P., Sikriwal, D., Rath, P., Choudhary, R. K. & Batra, J. K. Mycobacterium tuberculosis ClpC1: Characterization and role of the N-terminal domain in its function. Febs J 275, 6149–6158 (2008).Article

Kar，N.P.，Sikriwal，D.，Rath，P.，Choudhary，R.K。＆Batra，J.K。结核分枝杆菌ClpC1:N末端结构域在其功能中的表征和作用。Febs J 2756149–6158（2008）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Reheman, A. et al. Screening of microbial fermentation products for anti-M. tuberculosis activity. Anim. Open Access J. MDPI 12, 1947 (2022).

Reheman，A。等人。筛选抗结核分枝杆菌活性的微生物发酵产物。动画。开放获取J.MDPI 121947（2022）。

Google Scholar

谷歌学者

Hashimoto, T. et al. Identification, cloning and heterologous expression of biosynthetic gene cluster for desertomycin. J Antibiot (Tokyo) 73, 650–654 (2020).Article

Hashimoto，T。等人。沙漠霉素生物合成基因簇的鉴定，克隆和异源表达。J Antibiot（东京）73650-654（2020）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Kaweewan, I. et al. Isolation and structure determination of a new cytotoxic peptide, curacozole, from Streptomyces curacoi based on genome mining. J. Antibiot. (Tokyo) 72, 1–7 (2019).Article

Kaweewan，I。等人。基于基因组挖掘从库拉索酸链霉菌中分离和结构测定一种新的细胞毒性肽库拉索唑。J、抗生素。（东京）72,1-7（2019）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Morgan, K. D. et al. Incarnatapeptins A and B, nonribosomal peptides discovered using genome mining and (1)H/(15)N HSQC-TOCSY. Org. Lett. 22, 4053–4057 (2020).Article

Morgan，K.D.等人，使用基因组挖掘和（1）H/（15）N HSQC-TOCSY发现的非核糖体肽A和B。组织Lett。224053-4057（2020）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Thibault, P. Rifampicine, new antitubercular drug. La Presse Med. 75, 2816 (1967).CAS

Thibault，P。利福平，新型抗结核药物。《新闻医学》752816（1967）。中科院

Google Scholar

谷歌学者

Steenken, W. & Wolinsky, E. Isoniazid in experimental tuberculosis. Trans. Ann. Meet. Natl. Tuberc. Assoc. 48, 425–430 (1952).

Steenken，W。＆Wolinsky，E。异烟肼在实验性结核病中的作用。事务处理。安，见见。纳特尔。结核。协会48425-430（1952）。

Google Scholar

谷歌学者

Hinshaw, H. C., Pyle, M. M. & Feldman, W. H. Streptomycin in tuberculosis. Am. J. Med. 2, 429–435 (1947).Article

Hinshaw，H.C.，Pyle，M.M。和Feldman，W.H。链霉素在结核病中的作用。《美国医学杂志》2429-435（1947）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Cynamon, M. H., Klemens, S. P., Sharpe, C. A. & Chase, S. Activities of several novel oxazolidinones against Mycobacterium tuberculosis in a murine model. Antimicrob. Agents Chemother. 43, 1189–1191 (1999).Article

Cynamon，M.H.，Klemens，S.P.，Sharpe，C.A。＆Chase，S。几种新型恶唑烷酮在小鼠模型中对结核分枝杆菌的活性。抗菌剂。化学试剂。431189-1191（1999）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Lester, W. Jr., Salomon, A., Reimann, A. F., Shulruff, E. & Berg, G. S. Cycloserine therapy in tuberculosis in humans. Am. Rev. Tuberc. 74, 121–127 (1956).PubMed

。Am.Rev.Tubercum。74121-127（1956）。PubMed出版社

Google Scholar

谷歌学者

Weiss, W., Eisenberg, G. M. & Flippin, H. F. Kanamycin in pulmonary tuberculosis. Antibiot. Ann. 6, 711–712 (1958).PubMed

Weiss，W.，Eisenberg，G.M。和Flippin，H.F。卡那霉素在肺结核中的作用。抗生素。Ann.6711-712（1958）。PubMed出版社

Google Scholar

谷歌学者

Andries, K. et al. A diarylquinoline drug active on the ATP synthase of Mycobacterium tuberculosis. Science 307, 223–227 (2005).Article

Andries，K。等人。一种对结核分枝杆菌ATP合酶有活性的二芳基喹啉药物。科学307223-227（2005）。文章

ADS

CAS

中科院

PubMed

Google Scholar

谷歌学者

Gler, M. T. et al. Delamanid for multidrug-resistant pulmonary tuberculosis. New Engl. J. Med. 366, 2151–2160 (2012).Article

Gler，M.T.等人，Delamanid治疗耐多药肺结核。新英语。J、医学3662151-2160（2012）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Doggrell, S. A. New drugs being developed for the treatment of tuberculosis. Expert Opin. Investig. Drugs 14, 917–920 (2005).Article

。专家意见。调查。药物14917-920（2005）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Blin, K. et al. antiSMASH 70: New and improved predictions for detection, regulation, chemical structures and visualisation. Nucleic Acids Res. 51, W46-w50 (2023).Article

Blin，K.等人，《antiSMASH 70：检测、调节、化学结构和可视化的新预测和改进预测》。核酸研究51，W46-w50（2023）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Kumar, S., Stecher, G. & Tamura, K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 33, 1870–1874 (2016).Article

Kumar，S.，Stecher，G。和Tamura，K。MEGA7：用于较大数据集的分子进化遗传学分析7.0版。分子生物学。进化。331870–1874（2016）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Elbayaa, R. Y., Badr, M. H., Khalil, A. A. & Abdelhadi, M. Design, synthesis and evaluation of some novel pyrazoline derivatives as potential anti-inflammatory and antitumor agents. Drug Res. 63, 271–281 (2013).Article

Elbayaa，R.Y.，Badr，M.H.，Khalil，A.A。＆Abdelhadi，M。设计，合成和评估一些新型吡唑啉衍生物作为潜在的抗炎和抗肿瘤剂。。文章

CAS

中科院

Google Scholar

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Download referencesAcknowledgementsThis study was supported by the National Natural Science Foundation of China (Grant No. 32060023), Autonomous Region Graduate Research Innovation Project (Project Number: XJ2021G291) and The Third Xinjiang Scientific Expedition Program (Project Number: 2022xjkk0200).Author informationAuthors and AffiliationsState Key Laboratory Breeding Base for The Protection and Utilization of Biological Resources in TarimBasin Co-Funded By Xinjiang Production & Construction Corps and The Ministry of Science & Technology / College of Life Science and Technology, Tarim University, Alar, 843300, ChinaLei Wang & Chuanxing WanCollege of Animal Science and Technology, Tarim University, Alar, 843300, ChinaAikebaier RehemanAuthorsLei WangView author publicationsYou can also search for this author in.

下载参考文献致谢本研究得到了国家自然科学基金（批准号32060023），自治区研究生研究创新项目（项目编号：XJ2021G291）和第三次新疆科学考察项目（项目编号：2022XJK0200）的支持。作者信息作者和所属单位新疆生产建设兵团和科技部/塔里木大学生命科学与技术学院共同资助的塔里巴斯生物资源保护与利用国家重点实验室育种基地，阿拉尔，843300，中国雷王和川兴万塔里木大学动物科学与技术学院，阿拉尔，843300，中国艾克拜尔·雷赫曼作者雷王观点作者出版物您也可以在中搜索这位作者。

PubMed Google ScholarAikebaier RehemanView author publicationsYou can also search for this author in

PubMed Google ScholarAikebaier RehemanView作者出版物您也可以在

PubMed Google ScholarChuanxing WanView author publicationsYou can also search for this author in

PubMed Google ScholarChuanxing WanView作者出版物您也可以在

PubMed Google ScholarContributionsAll authors participated equally to data analysis, authoring, and revising the article. The find version of the manuscript has been reviewed and approved by all authors.Corresponding authorCorrespondence to

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Reprints and permissionsAbout this articleCite this articleWang, L., Reheman, A. & Wan, C. Discovery of anti-Mycobacterium tuberculosis desertomycins from Streptomyces flavofungini TRM90047 based on genome mining and HSQC-TOCSY.

转载和许可本文引用本文Wang，L.，Reheman，A。＆Wan，C。基于基因组挖掘和HSQC-TOCSY从黄霉菌TRM90047中发现抗结核分枝杆菌沙漠霉素。

Sci Rep 14, 17006 (2024). https://doi.org/10.1038/s41598-024-65702-0Download citationReceived: 01 March 2024Accepted: 24 June 2024Published: 24 July 2024DOI: https://doi.org/10.1038/s41598-024-65702-0Share this articleAnyone you share the following link with will be able to read this content:Get shareable linkSorry, a shareable link is not currently available for this article.Copy to clipboard.

科学报告1417006（2024）。https://doi.org/10.1038/s41598-024-65702-0Download引文接收日期：2024年3月1日接受日期：2024年6月24日发布日期：2024年7月24日OI：https://doi.org/10.1038/s41598-024-65702-0Share本文与您共享以下链接的任何人都可以阅读此内容：获取可共享链接对不起，本文目前没有可共享的链接。复制到剪贴板。

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KeywordsDesertomycinAnti-M. tb

关键词Desertomycinanti-M.tb

Genome miningHSQC-TOCSY

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