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register hereBackground: Echinococcus granulosus is a widespread zoonotic parasitic disease, significantly impacting human health and livestock development; however, no vaccine is currently available for humans. Our preliminary studies indicate that recombinant antigen P29 (rEg.P29) is a promising candidate for vaccine.

背景：细粒棘球蚴是一种广泛存在的人畜共患寄生虫病，对人类健康和牲畜发展产生重大影响；然而，目前还没有针对人类的疫苗。我们的初步研究表明，重组抗原P29（rEg。P29）是疫苗的有希望的候选者。

Methods: Sheep were immunized with rEg.P29, and venous blood was collected at various time points. Serum was isolated, and the presence of specific antibodies was detected using ELISA. We designed and synthesized a total of 45 B cell monopeptides covering rEg.P29 using the overlap method. ELISA was employed to assess the serum antibodies of the immunized sheep for recognition of these overlapping peptides, leading to the preliminary identification of B cell epitopes.

方法：用rEg.P29免疫绵羊，在不同时间点采集静脉血。分离血清，并使用ELISA检测特异性抗体的存在。我们使用重叠方法设计并合成了总共45个覆盖rEg.P29的B细胞单肽。ELISA用于评估免疫绵羊的血清抗体以识别这些重叠肽，从而初步鉴定B细胞表位。

Utilizing these identified epitopes, new single peptides were designed, synthesized, and used to optimize and confirm B-cell epitopes. Results: rEg.P29 effectively induces a sustained antibody response in sheep, particularly characterized by high and stable levels of IgG. Eight B-cell epitopes of were identified, which were mainly distributed in three regions of rEg.P29.

利用这些鉴定出的表位，设计，合成了新的单肽，并用于优化和确认B细胞表位。结果：rEg.P29有效诱导绵羊持续的抗体反应，特别是IgG水平高且稳定。鉴定出八个B细胞表位，主要分布在rEg.P29的三个区域。

Finally, three B cell epitopes were identified and optimized: rEg.P2971-90, rEg.P29151-175, and rEg.P29211-235. These optimized epitopes were well recognized by antibodies in sheep and mice, and the efficacy of these three epitopes significantly.

最后，鉴定并优化了三个B细胞表位：rEg.P2971-90，rEg.P29151-175和rEg.P29211-235。这些优化的表位在绵羊和小鼠中被抗体很好地识别，并且这三个表位的功效显着。