Mobile genetic element fluorescence in situ hybridization (MGE-FISH) creates spatial maps of target genes in microbiomes. We combine MGE-FISH with high phylogenetic resolution FISH (HiPR-FISH) to simultaneously map bacterial taxa and mobile genetic elements such as plasmids and phage, identifying their host taxa and revealing mobile genetic element spatial distribution..

移动遗传元件荧光原位杂交（MGE-FISH）创建微生物组中靶基因的空间图谱。我们将MGE-FISH与高系统发育分辨率FISH（HiPR-FISH）相结合，同时绘制细菌分类群和质粒和噬菌体等移动遗传元件，鉴定其宿主分类群并揭示移动遗传元件的空间分布。。

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Fig. 1: Mapping a phage gene found in a metagenome-assembled genome.

图1：绘制在宏基因组组装基因组中发现的噬菌体基因。

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Download referencesAdditional informationPublisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.This is a summary of: Grodner, B. et al. Spatial mapping of mobile genetic elements and their bacterial hosts in complex microbiomes.

下载参考其他信息出版商的注释Springer Nature对于已发布地图和机构隶属关系中的管辖权主张保持中立。这是Grodner，B。等人的总结。复杂微生物组中移动遗传元件及其细菌宿主的空间定位。

Nat. Microbiol. https://doi.org/10.1038/s41564-024-01735-5 (2024).Rights and permissionsReprints and permissionsAbout this articleCite this article Microscopy methods map mobile genetic elements and their bacterial hosts..

自然微生物。https://doi.org/10.1038/s41564-024-01735-5（2024年）。权利和许可打印和许可本文引用本文显微镜方法绘制移动遗传元素及其细菌宿主。。

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