CRISPR screening libraries for chicken and pig allow the study of gene disruptions in a single experiment to enable improved breeding and moreMOUNTAIN VIEW, Calif., Oct. 9, 2023 /PRNewswire/ -- Cellecta, Inc. today announced the release of new pooled, single-module, CRISPR genome-wide knockout libraries for chicken (Gallus gallus) and pig (Sus scrofa).

用于鸡和猪的CRISPR筛选文库允许在单个实验中研究基因破坏，以改善育种和加利福尼亚州的更多山景城，2023年10月9日/PRNewswire/-Cellecta，Inc。今天宣布发布新的用于鸡（Gallus Gallus）和猪（Sus scrofa）的合并，单模块，CRISPR全基因组敲除文库。

These new CRISPR libraries, with 4 guide RNAs (sgRNA) per gene, include:.

这些新的CRISPR文库，每个基因有4个指导RNA（sgRNA），包括：。

69,000 guides targeting 17,000 chicken genes

69000个指南针对17000个鸡基因

89,000 guides targeting 22,000 pig genes

针对22000头猪基因的89000份指南

And 1,000 non-targeting controls and 100 intron-targeting controls

以及1000个非目标控制和100个内含子目标控制

Continue Reading

继续阅读

Cellecta CRISPR Knockout Genome-Wide Pig Libraries for Genetic Screening

Cellecta CRISPR敲除全基因组猪文库用于遗传筛选

Cellecta CRISPR Knockout Genome-Wide Chicken Libraries for Genetic Screening

Cellecta CRISPR敲除全基因组鸡文库用于遗传筛选

In agricultural and dietary research, and other applications such as 'lab-grown' meat, genetic disruption screens are useful to identify genes responsible for advantageous traits such as pathogen resistance, improved breeding, and nutritional content.

在农业和饮食研究以及其他应用（如“实验室种植”的肉类）中，基因破坏筛选可用于鉴定负责有利性状的基因，如病原体抗性，改良育种和营养成分。

These CRISPR Chicken Genome 69K and Pig Genome 89K Knockout Libraries incorporate Cellecta's more effective HEAT1 sgRNA structure. The HEAT sgRNA structure significantly improves the signal-to-noise ratios in pooled screens by increasing the knockout efficiency of the guide RNA. 'We are pleased to offer one of the few commercially available, genome-wide CRISPR knockout libraries for researchers working with livestock and poultry,' stated Donato Tedesco, Ph.D., Cellecta Director of Research & Development.

这些CRISPR鸡基因组69K和猪基因组89K敲除文库结合了Cellecta更有效的HEAT1 sgRNA结构。热sgRNA结构通过提高指导RNA的敲除效率显着改善了合并筛选中的信噪比Cellecta研究与开发总监Tedesco博士说，我们很高兴为为为研究牲畜和家禽的研究人员提供为数不多的商业上可用的全基因组CRISPR敲除文库之一。

'CRISPR/Cas9 screening technology using single-module libraries provides large-scale data, which could simplify these complex breeding applications and make them more cost-effective and efficient,' he concluded.As with all its custom and pre-made library offerings, Cellecta includes full information and quality control data, including:.

他总结说：“使用单模块库的CRISPR/Cas9筛选技术提供了大规模的数据，可以简化这些复杂的育种应用，使其更具成本效益和效率。”。与所有定制和预制图书馆产品一样，Cellecta包含完整信息和质量控制数据，包括：。

representation levels of each sgRNA construct in the library as determined by next-generation sequencing (NGS)

通过下一代测序（NGS）确定文库中每个sgRNA构建体的表达水平

complete and confirmed sequence information of each sgRNA construct

完成并确认每个sgRNA构建体的序列信息

sgRNA sequence mutation rate as determined by NGS

NGS测定的sgRNA序列突变率

Materials supplied include:

提供的材料包括：

200 µg of plasmid library (enough for packaging of more than 2x109 lentiviral particles)

200μg质粒文库（足以包装超过2x109个慢病毒颗粒）

option to purchase each library as pre-packaged lentiviral particles

选择购买每个图书馆作为预包装慢病毒颗粒

In addition to the off-the-shelf CRISPR Chicken Genome and Pig Genome Knockout Libraries, Cellecta provides a suite of related products such as Cas9 Activity Test kits and NGS Library Prep kits specific to these libraries. Cellecta can also run the CRISPR screen as a service.Cellecta's pooled single-module CRISPR Chicken Genome 69K and Pig Genome 89K Knockout Libraries are available now.

除了现成的CRISPR鸡基因组和猪基因组敲除文库外，Cellecta还提供一套相关产品，如Cas9活性测试试剂盒和这些文库特有的NGS文库制备试剂盒。Cellecta也可以作为服务运行CRISPR屏幕。Cellecta的汇集单模块CRISPR鸡基因组69K和猪基因组89K敲除文库现在可用。

For more information, please visit https://cellecta.com/crispr-livestock-poultry-knockout-libraries1For more information on the improved HEAT design, please visit https://cellecta.com/improved-sgrna-designAbout Cellecta: Cellecta Inc., a trusted provider of genomic products and services, has successfully collaborated with the world's leading pharma, biotech, government, and academic institutions to advance drug target and biomarker discovery since 2006.

欲了解更多信息，请访问https://cellecta.com/crispr-livestock-poultry-knockout-libraries1For有关改进热设计的更多信息，请访问https://cellecta.com/improved-sgrna-designAboutCellecta:Cellecta Inc.是一家受信任的基因组产品和服务提供商，自2006年以来已成功与全球领先的制药，生物技术，政府和学术机构合作，推动药物靶标和生物标志物的发现。

Our recognized expertise in viral vector production, functional screening, cell engineering and multiplex qRT-PCR has given rise to a portfolio of offerings useful for loss-of-function and gain-of-function phenotypic screening, cell barcoding, cell engineering, targeted RNA-Seq expression & adaptive immune receptor profiling, and much more.Cellecta, Inc.

我们在病毒载体生产，功能筛选，细胞工程和多重qRT-PCR方面的公认专业知识为功能丧失和功能获得表型筛选，细胞条形码，细胞工程，靶向RNA-Seq表达和适应性免疫受体分析等等。Cellecta公司。

is headquartered in Mountain View, California. Further information about the company and its genomic products and services may be found online at www.cellecta.comCellecta, Inc.Paul Diehl, 650-938-4050 [email protected]orMedia: Ruth Mercado, 650-938-4080[email protected]SOURCE Cellecta, Inc..

总部设在加利福尼亚州山景城。有关公司及其基因组产品和服务的更多信息，请访问www.cellecta.comCellecta，Inc。Paul Diehl，650-938-4050[电子邮件保护]orMedia:Ruth Mercado，650-938-4080[电子邮件保护]SOURCE cellecta，Inc。。