Reproducibility and Rigour in Molecular Oncology

分子肿瘤学的可重复性和严谨性

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There is accumulating evidence indicating that ASS1 is closely related to tumors. No pan-cancer analysis of ASS1 was available. Here we explored the gene expression and survival analysis of ASS1 across thirty-three tumors based on the datasets of the TCGA (Cancer Genome Atlas), the GEO (Gene Expression Omnibus), and the GEPIA2 (Gene Expression Profiling Interactive Analysis, version 2).

越来越多的证据表明ASS1与肿瘤密切相关。没有可用的ASS1的泛癌分析。在这里，我们基于TCGA（癌症基因组图谱），GEO（基因表达综合）和GEPIA2（基因表达谱分析交互分析，版本）的数据集探索了ASS1在33个肿瘤中的基因表达和存活分析。2）。

ASS1 is highly expressed in most normal tissues and is related to the progression of some tumors. We also report ASS1 genetic alteration and their association with tumor prognosis and report differences in ASS1 phosphorylation sites between tumors and control normal tissues. ASS1 expression was associated with the infiltration of cancer-associated fibroblasts (CAFs) for the TCGA tumors of BRCA (Breast invasive carcinoma), CESC (Cervical squamous cell carcinoma and endocervical adenocarcinoma), COAD (Colon adenocarcinoma), ESCA (Esophageal carcinoma), SKCM (Skin cutaneous melanoma), SKCM-Metastasis, TGCT (Testicular germ cell tumors), and endothelial cell for the tumors of BRCA, BRCA-Basal, CESC, ESCA, KIRC (Kidney renal clear cell carcinoma), LUAD (Lung adenocarcinoma), LUSC (Lung squamous cell carcinoma), SKCM, SKCM-Metastasis, SKCM-Primary, STAD (Stomach adenocarcinoma), and TGCT.

ASS1在大多数正常组织中高度表达，并且与一些肿瘤的进展有关。我们还报告了ASS1基因改变及其与肿瘤预后的关系，并报告了肿瘤与对照正常组织之间ASS1磷酸化位点的差异。ASS1表达与BRCA（乳腺浸润癌），CESC（宫颈鳞状细胞癌和宫颈腺癌），COAD（结肠腺癌），ESCA（食管癌）的TCGA肿瘤的癌相关成纤维细胞（CAF）浸润有关。，SKCM（皮肤皮肤黑色素瘤），SKCM转移，TGCT（睾丸生殖细胞肿瘤），和内皮细胞用于BRCA，BRCA-Basal，CESC，ESCA，KIRC（肾肾透明细胞癌），LUAD（肺腺癌），LUSC（肺鳞状细胞癌），SKCM，SKCM转移，SKCM-原发性肿瘤，STAD（胃腺癌）和TGCT。

The KEGG and GO analysis were used to analyze ASS1-related signaling pathways. Finally, we used Huh7 cell line to verify the function of ASS1 in vitro. After ASS1 knockdown using small interfering RNA (siRNA), the proliferation and invasion of Huh7 were enhanced, cyclin D1 was up-regulated, and anti-apoptotic protein bax was downregulated, suggesting that ASS1 is a tumor suppressor gene in hepatocellular carcinoma.Our first pan-cancer study offers a relatively comprehensive understanding of the ro.

KEGG和GO分析用于分析ASS1相关的信号传导途径。最后，我们使用Huh7细胞系在体外验证ASS1的功能。在使用小干扰RNA（siRNA）敲低ASS1后，Huh7的增殖和侵袭增强，细胞周期蛋白D1上调，抗凋亡蛋白bax下调，表明ASS1是肝细胞癌中的肿瘤抑制基因。我们的第一个泛癌症研究提供了对ro的相对全面的了解。