Fast, streamlined workflows for Illumina®-based DNA and RNA library prep utilize a single protocol for a wide range of inputs

基于Illumina®的DNA和RNA文库制备的快速简化工作流程采用单一协议进行广泛的输入

IPSWICH, Mass., Nov. 3, 2023 /PRNewswire/ -- New England Biolabs (NEB®) today announced the release of the NEBNext UltraExpress DNA and RNA Library Prep Kits for next generation sequencing on the Illumina platform. These new kits are designed to generate high-quality, high-yield libraries with a faster, easier workflow, enabling RNA library prep in a single day, and DNA library prep in under two hours.

马萨诸塞州伊普斯威奇，2023年11月3日/PRNewswire/-新英格兰生物实验室（NEB®）今天宣布发布NEBNext UltraExpress DNA和RNA文库制备试剂盒，用于Illumina平台上的下一代测序。这些新试剂盒旨在通过更快，更轻松的工作流程生成高质量，高产的文库，在一天内完成RNA文库制备，并在两小时内完成DNA文库制备。

The kits are suitable for a wide range of inputs (25 -250 ng total RNA and 10 – 200 ng mechanically sheared DNA) and utilize a single protocol that uses the same adaptor concentration and PCR cycle number for all input amounts. The workflows are automation friendly and minimize consumables waste..

该试剂盒适用于多种输入（25-250 ng总RNA和10-200 ng机械剪切DNA），并采用单一方案，对所有输入量使用相同的接头浓度和PCR循环数。工作流程自动化友好，最大限度地减少耗材浪费。。

'With labs doing sequencing more frequently and at higher throughputs than before, researchers need ways to do sequencing faster, while maintaining high-quality results,' said Fiona Stewart, Associate Director, NEBNext Portfolio Management, at NEB. 'We designed the NEBNext UltraExpress workflows to meet the need for substantially faster and easier library prep of samples within a standard input range.

NEBNext投资组合管理副总裁Fiona Stewart说：“由于实验室比以前更频繁，产量更高，测序速度更快，同时保持高质量的结果，在NEB。”我们设计了NEBNext UltraExpress工作流程，以满足在标准输入范围内显着更快更轻松地准备样品库的需求。

The streamlined workflows, which include a single protocol for all inputs, with the same adaptor concentration and PCR cycle number, save time and eliminate end-user optimization. Additionally, we've incorporated master mix reagents, reduced incubation times, and have fewer cleanup steps so that researchers can complete the DNA workflow in less than two hours and the RNA library prep workflow in three hours.'.

简化的工作流程，包括所有输入的单一协议，具有相同的适配器浓度和PCR循环次数，节省时间并消除最终用户的优化。此外，我们还采用了master mix试剂，缩短了孵育时间，并且清理步骤更少，因此研究人员可以在不到两个小时的时间内完成DNA工作流程，并在三个小时内完成RNA文库制备工作流程。

Early access user Ravi Ranjan, Ph.D., a director at the Genomics Resource Laboratory at the University of Massachusetts Amherst, reported that he was pleased with the single-tube master mixes, reduced bead cleanups, and RNA input amounts, along with the time savings.

马萨诸塞州阿默斯特大学基因组学资源实验室主任Ravi Ranjan博士报告说，他对单管主混合物，减少珠子清理和RNA输入量以及时间节省感到满意。

'I prepared RNA seq libraries from fungi total RNA in conjunction with the NEBNext Poly(A) mRNA Magnetic Isolation Module protocol. The workflow was easy to follow with great time savings, and libraries were of great quality.'

我从真菌总RNA中结合NEBNext Poly（A）mRNA磁分离模块方案制备了RNA-seq文库。工作流程易于遵循，节省了大量时间，图书馆质量很高

The RNA kit creates high-quality directional RNA libraries when paired with poly(A) mRNA enrichment or rRNA depletion kits. The overall plastics usage also decreases significantly. Compared to the NEBNext® Ultra™ II Directional RNA kit, NEBNext UltraExpress RNA uses 21% fewer tubes and 25% fewer tips.

当与poly（A）mRNA富集或rRNA耗尽试剂盒配对时，RNA试剂盒创建高质量的定向RNA文库。塑料的总体使用量也显着下降。与NEBNext®Ultra相比™ II定向RNA试剂盒，NEBNext UltraExpress RNA使用的管减少了21%，尖端减少了25%。

The NEBNext UltraExpress DNA kit also saves on consumables (33% fewer tubes and 32% fewer tips) and is a single-tube workflow. For both kits, flexibility is enabled with simple guidelines for customized protocols, if desired, and automation-friendly transfer volumes..

NEBNext UltraExpress DNA试剂盒还可以节省耗材（管减少33%，尖端减少32%），并且是单管工作流程。对于这两个套件，都可以通过简单的定制协议指南（如果需要）和自动化友好的传输量来实现灵活性。。

Learn more about the NEBNext UltraExpress DNA and RNA Kits.

了解有关NEBNext UltraExpress DNA和RNA试剂盒的更多信息。

About New England Biolabs:Established in the mid 1970's, New England Biolabs, Inc. (NEB) is the industry leader in the discovery and production of enzymes for molecular biology applications and now offers the largest selection of recombinant and native enzymes for genomic research. NEB continues to expand its product offerings into areas related to PCR, gene expression, sample preparation for next generation sequencing, synthetic biology, glycobiology, epigenetics and RNA analysis.

关于新英格兰生物实验室：成立于1970年代中期，新英格兰生物实验室公司（NEB）是分子生物学应用酶的发现和生产行业领导者，现在为基因组研究提供最大的重组和天然酶选择。NEB继续将其产品扩展到与PCR，基因表达，下一代测序样品制备，合成生物学，糖生物学，表观遗传学和RNA分析相关的领域。

Additionally, NEB is focused on strengthening alliances that enable new technologies to reach key market sectors, including molecular diagnostics development. New England Biolabs is a privately held company, headquartered in Ipswich, MA, and has extensive worldwide distribution through a network of exclusive distributors, agents and seven subsidiaries located in Canada, China, France, Germany, Japan, Singapore and the UK.

此外，NEB专注于加强联盟，使新技术能够进入关键市场领域，包括分子诊断学开发。新英格兰生物实验室是一家私营公司，总部设在马萨诸塞州伊普斯威奇，通过位于加拿大，中国，法国，德国，日本，新加坡和英国的独家分销商，代理商和七家子公司网络在全球广泛分销。

For more information about New England Biolabs visit www.neb.com..

有关新英格兰生物实验室的更多信息，请访问www.neb.com。。

NEB®, NEW ENGLAND BIOLABS®, and NEBNext® are registered trademarks of New England Biolabs, Inc.NEBNEXT ULTRAEXPRESS™ and ULTRA™ are trademarks of New England Biolabs, Inc.ILLUMINA® is a registered trademark of Illumina, Inc.

NEB®，NEW ENGLAND BIOLABS®和NEBNext®是NEW ENGLAND BIOLABS，Inc.NEBNext ULTRAEXPRESS的注册商标™ 和超™ 是New England Biolabs，Inc.ILLUMINA®的商标是ILLUMINA，Inc。的注册商标。

SOURCE New England Biolabs

来源新英格兰生物实验室