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register hereMore affordable and effective vaccines against bacterial meningitis caused by Neisseria meningitidis serogroup B are still required for global prevention. We have previously shown that modified outer membrane vesicles (mOMVs) from commensal Neisseria cinerea can be used as a platform to induce immune responses against meningococcal antigens.

在这里注册全球预防仍然需要更实惠和有效的疫苗来预防由脑膜炎奈瑟菌B血清群引起的细菌性脑膜炎。我们之前已经表明，来自共生的灰葡萄球菌的修饰的外膜囊泡（MOMV）可以用作诱导针对脑膜炎球菌抗原的免疫应答的平台。

The aim of the present study was to use a combination of two genetically engineered mOMVs to express multiple antigens from N. meningitidis known to be involved in protective immunity to meningococcal meningitis (different variants of factor H binding protein (fHbp), Neisseria Heparin Binding Antigen (NHBA) and Neisseria Adhesin A (NadA)).

本研究的目的是使用两种基因工程MOMV的组合来表达已知参与脑膜炎球菌脑膜炎保护性免疫的脑膜炎奈瑟菌的多种抗原（H因子结合蛋白（fHbp），奈瑟菌肝素结合抗原（NHBA）的不同变体）和奈瑟菌粘附素a（NadA））。

Antigen expression in the mOMVs was confirmed by Western blotting; detoxification of the lipooligosaccharide (LOS) was confirmed by measuring human toll like receptor 4 (hTLR4) activation using in vitro cell assays. Mice immunised with a combination of two mOMVs expressing fHbp, NHBA and NadA produced antibodies to all the antigens.

通过蛋白质印迹证实了MOMV中的抗原表达；通过使用体外细胞测定法测量人toll样受体4（hTLR4）的活化，证实了脂寡糖（LOS）的解毒作用。用表达fHbp，NHBA和NadA的两种MOMV的组合免疫的小鼠产生针对所有抗原的抗体。

Furthermore, serum bactericidal activity (SBA) was induced by the immunisation, with mOMVs expressing NadA displaying high SBA titres against a nadA + MenB strain. The work highlights the potential of mOMVs from N. cinerea to induce functional immune responses against multiple antigens involved in the protective immune response to meningococcal disease..

此外，通过免疫诱导血清杀菌活性（SBA），表达NadA的MOMV显示出针对NadA+MenB菌株的高SBA滴度。这项工作突出了来自灰葡萄球菌的MOMV诱导针对涉及脑膜炎球菌疾病保护性免疫应答的多种抗原的功能性免疫应答的潜力。。