草酸钙处理TCMK-1细胞和巨噬细胞通过外泌体相互作用的关键分子和途径的鉴定-动脉网

The identification of key molecules and pathways in the crosstalk of calcium oxalate-treated TCMK-1 cells and macrophage via exosomes

Nature 等信源发布 2024-09-09 06:20

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AbstractThe interplay between crystals and epithelial cells forms the cornerstone of kidney stone development, communication between epithelial cells and macrophages emerging as a pivotal role in this process. We conducted next-generation sequencing on the secreted exosomes of TCMK-1 cells treated with calcium oxalate monohydrate (OX_EXO) or controls (NC_EXO), and on the macrophage cell line RAW264.7 stimulated with OX_EXO or NC_EXO, followed by validation of differentially expressed target proteins and miRNAs through Western blot and PCR.

摘要晶体和上皮细胞之间的相互作用是肾结石发展的基石，上皮细胞和巨噬细胞之间的通讯在这一过程中起着关键作用。我们对用草酸钙一水合物（OX\U-EXO）或对照（NC\U-EXO）处理的TCMK-1细胞的分泌外泌体以及用OX\U-EXO或NC\U-EXO刺激的巨噬细胞系RAW264.7进行了下一代测序，然后通过蛋白质印迹和PCR验证差异表达的靶蛋白和miRNA。

UPSET plots were employed to identify genes co-targeted by exosomal miRNAs. Various bioinformatic analyses were employed to predict potential mechanisms of the dysregulated genes. We integrated sequencing data from the GEO database, and validated findings using clinical patient urine and kidney tissues.

扰乱图用于鉴定外泌体miRNA共靶向的基因。各种生物信息学分析被用来预测失调基因的潜在机制。我们整合了GEO数据库中的测序数据，并使用临床患者尿液和肾脏组织验证了发现。

We identified 665 differentially expressed exosomal miRNAs between OX_EXO and NC_EXO. Among the top 10 down-regulated miRNAs, the most targeted genes were AAK1 and NUFIP2, whereas PLCB1 was significantly targeted among the top 10 up-regulated miRNAs. In clinical specimens, we confirmed the differential expressions of five homologous miRNAs, as well as CNOT3, CNCNA1C, APEX1, and TMEM199.

我们在OX\U EXO和NC\U EXO之间鉴定了665个差异表达的外泌体miRNA。在前10个下调的miRNA中，最靶向的基因是AAK1和NUFIP2，而PLCB1在前10个上调的miRNA中显着靶向。在临床标本中，我们证实了五种同源miRNA以及CNOT3，CNCNA1C，APEX1和TMEM199的差异表达。

In conclusion, treatment of TCMK-1 cells with calcium oxalate significantly alerted the expression profile of exosomal miRNAs, subsequently influencing gene expression in macrophages, thereby modulating the processes of kidney stone formation..

总之，用草酸钙处理TCMK-1细胞可显着提醒外泌体miRNA的表达谱，从而影响巨噬细胞中的基因表达，从而调节肾结石的形成过程。。

IntroductionKidney stones are a common urinary system disorder that is increasingly in prevalence due to dietary, environmental, and lifestyle changes1,2. The development of percutaneous nephrolithotomy and ureteroscopy has significantly enhanced the efficiency and safety of stone treatment and removal3,4.

引言肾结石是一种常见的泌尿系统疾病，由于饮食，环境和生活方式的改变，其患病率越来越高1,2。经皮肾镜取石术和输尿管镜检查的发展显着提高了结石治疗和清除的效率和安全性3,4。

However, kidney stones have a high recurrence rate, with approximately 50% of patients experiencing a recurrent episode within five years5, emphasizing the importance of preventive measures and long-term patient management. Early diagnosis and lifestyle modifications are crucial to prevent calculi formation.Exosomes are extracellular vesicles approximately 30–150 nm in diameter and surrounded by a double lipid membrane.

然而，肾结石的复发率很高，大约50%的患者在五年内复发5，强调了预防措施和长期患者管理的重要性。。外泌体是直径约30-150 nm的细胞外囊泡，被双脂质膜包围。

Exosomes selectively package and deliver a range of bioactive materials such as proteins, RNA, and lipids to target cells6, influencing the behavior and function of the recipient cells. Exosomes are important mediators of intercellular communications and play a key role in a variety of biological processes, such as immune responses and disease progression7.

外泌体选择性地包装并将一系列生物活性物质如蛋白质，RNA和脂质递送至靶细胞6，影响受体细胞的行为和功能。外泌体是细胞间通讯的重要介质，在多种生物过程中发挥关键作用，如免疫反应和疾病进展7。

The production and secretion of exosomes represent important mechanisms of intercellular communication8.Among the types of kidney stones, the primary component of stone is calcium oxalate monohydrate (COM). Stone formation is a complex process, that involves several mechanisms, including crystal supersaturation, crystal-cell adhesion, crystal deposition, growth, and crystal-cell interactions, that contribute to the retention of crystals in the kidney damage9,10.

外泌体的产生和分泌代表了细胞间通讯的重要机制8。在肾结石的类型中，结石的主要成分是草酸钙一水合物（COM）。结石形成是一个复杂的过程，涉及多种机制，包括晶体过饱和，晶体细胞粘附，晶体沉积，生长和晶体细胞相互作用，这些机制有助于晶体在肾脏损伤中的保留9,10。

These crystals subsequently induce cytotoxicity and dysfunction in renal tubular epithelial cells (TECs), which in turn increases crystal formation and adhesion11. MicroRNAs (miRNAs) are short, non-coding RNAs comprised of 20–25 nucleotides .

这些晶体随后在肾小管上皮细胞（TECs）中诱导细胞毒性和功能障碍，进而增加晶体形成和粘附11。微小RNA（miRNA）是由20-25个核苷酸组成的短的非编码RNA。

Data availability

数据可用性

Data is provided within the manuscript or supplementary information files. The datasets used and analyzed in this study are available from the corresponding author on reasonable request.

数据在手稿或补充信息文件中提供。本研究中使用和分析的数据集可根据合理要求从通讯作者处获得。

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Download referencesAcknowledgementsWe thank Bullet Edits Limited for the linguistic editing and proofreading of the manuscript.FundingThis work was supported by National Natural Science Foundation of China (82170775, and 82100806).Author informationAuthor notesThese authors contributed equally: Yushi Sun, Bojun Li and Xiangjun Zhou.Authors and AffiliationsDepartment of Urology, Renmin Hospital of Wuhan University, No.238 Jiefang Road, Wuchang District, Wuhan, 430060, Hubei, People’s Republic of ChinaYushi Sun, Bojun Li, Xiangjun Zhou, Ting Rao & Fan ChengAuthorsYushi SunView author publicationsYou can also search for this author in.

下载参考文献致谢我们感谢Bullet Edits Limited对稿件进行语言编辑和校对。资助这项工作得到了国家自然科学基金（82170775和82100806）的支持。作者信息作者注意到这些作者做出了同样的贡献：孙玉石，李伯军和周向军。。

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PubMed Google ScholarContributionsConceptualization, Yushi Sun and Bojun Li; Formal analysis, Yushi Sun and Bojun Li; Funding acquisition, Fan Cheng; Methodology, Bojun Li; Supervision, Rao Ting and Fan Cheng; Validation, Yushi Sun, Bojun Li and Xiangjun Zhou; Visualization, Yushi Sun; Writing—original draft, Yushi Sun, Bojun Li and Fan Cheng; Writing—review & editing, Yushi Sun, Bojun Li, Xiangjun Zhou and Fan Cheng.Corresponding authorsCorrespondence to.

PubMed谷歌学术贡献概念化，孙玉石和李伯军；；资金收购，范成；方法论，李伯军；监督，饶婷和范成；验证，孙玉石，李伯军和周向军；可视化，孙玉石；撰写原稿，孙玉石，李伯军和范成；写作评论与编辑，孙玉石，李伯军，周向军，范成。通讯作者通讯。

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Competing interests

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The authors declare no competing interests.

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Ethical approval

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The clinical specimen collection (approval number: WDRY2021-KS047) was approved by the Ethics Committee of the Renmin Hospital of Wuhan University. Confirms that all experiments were performed in accordance with relevant named guidelines and regulations. Confirms that informed consent was obtained from all participants and/or their legal guardians..

临床标本采集（批准号：WDRY2021-KS047）经武汉大学人民医院伦理委员会批准。确认所有实验均按照相关命名指南和法规进行。确认已获得所有参与者和/或其法定监护人的知情同意。。

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Reprints and permissionsAbout this articleCite this articleSun, Y., Li, B., Zhou, X. et al. The identification of key molecules and pathways in the crosstalk of calcium oxalate-treated TCMK-1 cells and macrophage via exosomes.

转载和许可本文引用本文Sun，Y.，Li，B.，Zhou，X。等人通过外泌体鉴定草酸钙处理的TCMK-1细胞和巨噬细胞串扰中的关键分子和途径。

Sci Rep 14, 20949 (2024). https://doi.org/10.1038/s41598-024-71755-yDownload citationReceived: 17 May 2024Accepted: 30 August 2024Published: 09 September 2024DOI: https://doi.org/10.1038/s41598-024-71755-yShare this articleAnyone you share the following link with will be able to read this content:Get shareable linkSorry, a shareable link is not currently available for this article.Copy to clipboard.

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KeywordsExosomesMiRNAMacrophageBiomarkers in kidney stonesTranscriptomics of kidney stones

关键词肾结石中的sosomesmiRNamacrophagebiomarkers肾结石的描述组学

Subjects

主题

BiomarkersImmunologyMolecular medicineNephrologySequencingUrology

生物标志物免疫分子医学肾脏测序生物学

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