藏绵羊睾丸和附睾端粒细胞的形态特征及分布鉴定-动脉网

Morphological characteristics and distribution identification of telocytes in Tibetan sheep testis and epididymis

Nature 等信源发布 2024-10-01 16:44



可切换为仅中文







AbstractTelocytes (TCs) are a type of stromal cell discovered in the various organs of different animals and have many potential functions, including angiogenesis, signalling, and substance transport. However, the TCs have not been detected in the testis or epididymis of Tibetan sheep. This study investigated the position, characteristics, and distribution of TCs in the testis and epididymis of Tibetan sheep using transmission electron microscopy (TEM), toluidine blue staining, immunohistochemistry, and double immunofluorescence to elucidate their possible functions.

摘要细胞（TC）是在不同动物的各种器官中发现的一种基质细胞，具有许多潜在功能，包括血管生成，信号传导和物质转运。然而，在藏绵羊的睾丸或附睾中尚未检测到TC。本研究使用透射电子显微镜（TEM），甲苯胺蓝染色，免疫组织化学和双重免疫荧光研究了藏绵羊睾丸和附睾中TC的位置，特征和分布，以阐明其可能的功能。

TEM revealed that TCs were often found near basement membranes and capillaries and were characterised by large nuclei, elongated cytoplasmic protrusions, and many secretory vesicles. We also observed via toluidine staining that TCs were present near basement membrane and interstitial capillaries. Immunohistochemistry and double immunofluorescence revealed the positive expression of CD117, vimentin, platelet derived growth factor receptor α(PDGFRα), PDGFRα + CD117, and PDGFRα + vimentin in TCs.

透射电镜显示，TC常在基底膜和毛细血管附近发现，其特征是细胞核大，细胞质突起细长，分泌囊泡多。我们还通过甲苯胺酮染色观察到TC存在于基底膜和间质毛细血管附近。免疫组织化学和双重免疫荧光显示TCs中CD117，波形蛋白，血小板衍生生长因子受体α（PDGFRα），PDGFRα+CD117和PDGFRα+vimentin的阳性表达。

Additionally, we inferred that TCs participates in the formation of the blood–testis and blood–epididymis barriers, as well as in material transport and a stable microenvironment. This study presents the first evidence of the presence of TCs near the basement membrane and blood vessels in the testis and epididymis of Tibetan sheep.

此外，我们推断TCs参与了血液-睾丸和血液-附睾屏障的形成，以及物质运输和稳定的微环境。这项研究首次证明了藏绵羊睾丸和附睾中基底膜和血管附近存在TC。

These findings provide new insights into the function of TCs in the reproductive systems of plateau animals..

这些发现为TC在高原动物生殖系统中的功能提供了新的见解。。

IntroductionTelocytes (TCs) were discovered by Popescu et al. in 2005 using transmission electron microscopy (TEM) and were named interstitial Cajal-like cells because their structure is similar to that of the interstitial cells of Cajal1. In 2010, researchers renamed them “telocytes” based on their morphology using the Greek suffix “telos”2.

引言细胞（TC）由Popescu等人于2005年使用透射电子显微镜（TEM）发现，由于其结构与Cajal1的间质细胞相似，因此被命名为间质Cajal样细胞。2010年，研究人员根据它们的形态，使用希腊语后缀“telos”2将其重命名为“telocytes”。

The TCs have diverse morphologies, such as spindle, pear, and pike3, and unique cell extensions called telopodes (TPs) that distinguish them from other cells4. The TPs extend from the cell body with fine or expanded segments enriched with mitochondria, endoplasmic reticulum, and secretory vesicles that form mesenchymal networks that facilitate intercellular communication2,5.

TC具有多种形态，例如纺锤体，梨和pike3，以及称为端足类（TPs）的独特细胞延伸，将它们与其他细胞区分开4。TPs从细胞体延伸，具有富含线粒体，内质网和分泌囊泡的精细或扩展片段，形成促进细胞间通讯的间充质网络2,5。

It is currently believed that TCs play a role in cellular signal transduction6, maintenance of tissue homeostasis7, and inhibition of cellular senescence8, which are involved in the development of different diseases9.For many years, studies on TCs have been mainly based on the observation of their ultrastructure by TEM; thus, TEM remains the “gold standard” for distinguishing TCs from other interstitial cells10,11.

目前认为，TC在细胞信号转导6，维持组织稳态7和抑制细胞衰老8中发挥作用，这些都参与了不同疾病的发展9。多年来，对TC的研究主要基于通过TEM观察其超微结构；因此，TEM仍然是区分TC与其他间质细胞的“金标准”10,11。

Immunofluorescence (IF) and immunohistochemistry (IHC) are key approaches for TCs identification. The most commonly used markers are stem cell growth factor receptor (C-Kit, also known as CD117), vimentin and platelet derived growth factor receptor α (PDGFRα). The CD117 is a marker of stem cells in the heart12 and is considered an effective marker for TCs13.

免疫荧光（IF）和免疫组织化学（IHC）是鉴定TC的关键方法。最常用的标记物是干细胞生长因子受体（C-Kit，也称为CD117），波形蛋白和血小板衍生生长因子受体α（PDGFRα）。CD117是心脏干细胞的标志物12，被认为是TCs13的有效标志物。

Thus, the CD117 expression by TCs suggests that it may be associated with the haematopoietic system. Vimentin, a marker of interstitial cells, is an important cytoskeletal protein closely associated with tumour cell growth, invasion, and metastasis14. The PDGFRα is a tyrosine kinase receptor, bot.

因此，TCs的CD117表达表明它可能与造血系统有关。波形蛋白是间质细胞的标志物，是一种重要的细胞骨架蛋白，与肿瘤细胞的生长，侵袭和转移密切相关14。PDGFRα是酪氨酸激酶受体bot。

Data availability

数据可用性

The datasets used and analysed during the current study available from the corresponding author on reasonable request.

本研究期间使用和分析的数据集可根据合理要求从通讯作者处获得。

ReferencesPopescu, L. M. et al. Interstitial cells of Cajal in pancreas. J. Cell. Mol. Med.9, 169–190 (2005).Article

参考文献Popescu，L.M。等人胰腺中Cajal的间质细胞。J、细胞。《分子医学》9169-190（2005）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Popescu, L. M. & Faussone-Pellegrini, M. S. TELOCYTES—a case of serendipity: The winding way from Interstitial Cells of Cajal (ICC), via interstitial Cajal-Like cells (ICLC) to TELOCYTES. J. Cell. Mol. Med.14, 729–740 (2010).Article

Popescu，L.M。＆Faussone Pellegrini，M.S。TELOCYTES-一个偶然的案例：从Cajal间质细胞（ICC），通过间质Cajal样细胞（ICLC）到TELOCYTES的曲折之路。J、细胞。Mol.Med.14729–740（2010）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Aleksandrovych, V., Walocha, J. A. & Gil, K. Telocytes in female reproductive system (human and animal). J. Cell. Mol. Med.20, 994–1000 (2016).Article

Aleksandrovych，V.，Walocha，J.A。＆Gil，K。雌性生殖系统（人和动物）中的Telocytes。J、细胞。Mol.Med.20994-1000（2016）。文章

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Varga, I. et al. The functional morphology and role of cardiac telocytes in myocardium regeneration. Can. J. Physiol. Pharmacol.94, 1117–1121 (2016).Article

Varga，I。等人。心脏终末细胞在心肌再生中的功能形态和作用。可以。J、生理学。Pharmacol.941117-1121（2016）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Abdel-Maksoud, F. M., Abd-Elhafeez, H. H. & Soliman, S. A. Morphological changes of telocytes in camel efferent ductules in response to seasonal variations during the reproductive cycle. Sci. Rep.9, 4507 (2019).Article

Abdel Maksoud，F.M.，Abd Elhafeez，H.H。＆Soliman，S.A。骆驼传出小管中端细胞的形态变化，以响应生殖周期中的季节变化。科学。Rep.94507（2019）。文章

ADS

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Song, D. et al. Comparison of chromosome 4 gene expression profile between lung telocytes and other local cell types. J. Cell. Mol. Med.20, 71–80 (2016).Article

Song，D.等人。肺终末细胞和其他局部细胞类型之间4号染色体基因表达谱的比较。J、细胞。Mol.Med.20,71-80（2016）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Zheng, Y. et al. Genetic comparison of mouse lung telocytes with mesenchymal stem cells and fibroblasts. J. Cell. Mol. Med.17, 567–577 (2013).Article

郑，Y。等。小鼠肺终末细胞与间充质干细胞和成纤维细胞的遗传比较。J、细胞。Mol.Med.17567-577（2013）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Zheng, Y. et al. Protein profiling of human lung telocytes and microvascular endothelial cells using iTRAQ quantitative proteomics. J. Cell. Mol. Med.18, 1035–1059 (2014).Article

Zheng，Y。等人。使用iTRAQ定量蛋白质组学对人肺终末细胞和微血管内皮细胞进行蛋白质分析。J、细胞。Mol.Med.181035-1059（2014）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Varga, I. et al. Recently discovered interstitial cell Population of telocytes: Distinguishing facts from Fiction regarding their role in the pathogenesis of diverse diseases called telocytopathies. Med. (Kaunas)55 (2019).Yang, D. et al. Morphological and histochemical identification of telocytes in adult yak epididymis.

Varga，I。等人最近发现了间质细胞群的端细胞：将事实与虚构区分开来，以了解它们在称为端细胞病的多种疾病的发病机理中的作用。医学（考纳斯）55（2019）。Yang，D。等人。成年牦牛附睾中端粒细胞的形态学和组织化学鉴定。

Sci. Rep.13, 5295 (2023).Article .

科学。Rep.135295（2023）。文章。

ADS

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Yang, J. et al. Ultrastructure damage of oviduct telocytes in rat model of acute salpingitis. J. Cell. Mol. Med.19, 1720–1728 (2015).Article

Yang，J。等人。急性输卵管炎大鼠模型中输卵管末端细胞的超微结构损伤。J、细胞。Mol.Med.191720–1728（2015）。文章

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Janicki, J. S., Brower, G. L. & Levick, S. P. The emerging prominence of the cardiac mast cell as a potent mediator of adverse myocardial remodeling. Methods Mol. Biol.1220, 121–139 (2015).Article

Janicki，J.S.，Brower，G.L。和Levick，S.P。心脏肥大细胞作为不良心肌重塑的有效介质的新兴突出。方法Mol.Biol.1220121-139（2015）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Ravalli, S. et al. Morphological evidence of telocytes in skeletal muscle interstitium of exercised and sedentary rodents. Biomedicines. 9, 807 (2021).Article

Ravalli，S.等人。运动和久坐啮齿动物骨骼肌间质中端粒细胞的形态学证据。生物医学。9807（2021）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Tadokoro, A. et al. Vimentin regulates invasiveness and is a poor prognostic marker in non-small cell Lung Cancer. Anticancer Res.36, 1545–1551 (2016).CAS

Tadokoro，A。等人波形蛋白调节侵袭性，是非小细胞肺癌预后不良的标志物。抗癌研究361545-1551（2016）。中科院

PubMed

Google Scholar

谷歌学者

Kondo, A. & Kaestner, K. H. Emerging diverse roles of telocytes. Development146 (2019).Liu, Y. et al. Identification and characterization of telocytes in rat testis. Aging (Albany NY)11, 5757–5768 (2019).Article

Kondo，A。＆Kaestner，K.H。新兴的端粒细胞的多种作用。发展146（2019）。Liu，Y。等人。大鼠睾丸中端粒细胞的鉴定和表征。老龄化（纽约州奥尔巴尼）115757-5768（2019）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Pawlicki, P. et al. Telocytes in the mouse testicular interstitium: Implications of G-protein-coupled estrogen receptor (GPER) and estrogen-related receptor (ERR) in the regulation of mouse testicular interstitial cells. Protoplasma. 256, 393–408 (2019).Article

Pawlicki，P。等人。小鼠睾丸间质中的终末细胞：G蛋白偶联雌激素受体（GPER）和雌激素相关受体（ERR）在小鼠睾丸间质细胞调节中的意义。。256393-408（2019）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Sha, Y. et al. Multi-omics revealed rumen microbiota metabolism and host immune regulation in tibetan sheep of different ages. Front. Microbiol.15, 1339889 (2024).Article

Sha，Y。等人。多组学揭示了不同年龄藏绵羊瘤胃微生物群代谢和宿主免疫调节。正面。Microbiol.151339889（2024）。文章

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Rosa, I. et al. Immunohistochemical and ultrastructural identification of telocytes in the lamina propria of human vaginal mucosa. Acta Histochem.125, 152094 (2023).Article

Rosa，I.等人。人阴道粘膜固有层中端细胞的免疫组织化学和超微结构鉴定。组织化学学报125152094（2023）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Etcharren, V., Mouguelar, H. & Aguilar Valenciano, J. J. Identification of telocytes in the oviduct of the mare. Theriogenology205, 18–26 (2023).Article

Etcharren，V.，Mouguelar，H。和Aguilar Valenciano，J。J。鉴定母马输卵管中的端细胞。Theriogenology205,18-26（2023）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Qi, G. et al. Telocytes in the human kidney cortex. J. Cell. Mol. Med.16, 3116–3122 (2012).Article

Qi，G。等人。人肾皮质中的终末细胞。J、细胞。Mol.Med.163116–3122（2012）。文章

CAS

中科院

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Manole, C. G. & Simionescu, O. The cutaneous telocytes. Adv. Exp. Med. Biol.913, 303–323 (2016).Article

Manole，C.G。和Simionescu，O。皮肤终端细胞。Adv.Exp.Med.Biol.913303–323（2016）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Ma, Y., Ma, Q. W., Sun, Y. & Chen, X. F. The emerging role of extracellular vesicles in the testis. Hum. Reprod.38, 334–351 (2023).Article

Ma，Y.，Ma，Q.W.，Sun，Y。＆Chen，X.F。细胞外囊泡在睾丸中的新兴作用。哼，《报告》38334-351（2023）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Chen, Q. & Holt, W. V. Extracellular vesicles in the male reproductive tract of the softshell turtle. Reprod. Fertil. Dev.33, 519–529 (2021).Article

Chen，Q。＆Holt，W.V。软壳龟雄性生殖道中的细胞外囊泡。重新编程。费尔蒂尔。Dev.33519-529（2021）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Arafat, E. A. Ultrastructural and immunohistochemical characteristics of telocytes in the skin and skeletal muscle of newborn rats. Acta Histochem.118, 574–580 (2016).Article

Arafat，E.A。新生大鼠皮肤和骨骼肌中端粒细胞的超微结构和免疫组织化学特征。组织化学学报118574-580（2016）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Rosa, I., Marini, M., Guasti, D., Ibba-Manneschi, L. & Manetti, M. Morphological evidence of telocytes in human synovium. Sci. Rep.8, 3581 (2018).Article

Rosa，I.，Marini，M.，Guasti，D.，Ibba Manneschi，L。＆Manetti，M。人类滑膜中端细胞的形态学证据。科学。Rep.83581（2018）。文章

ADS

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Suciu, L. et al. Telocytes in human term placenta: Morphology and phenotype. Cells Tissues Organs.192, 325–339 (2010).Article

Suciu，L。等人。人足月胎盘中的端细胞：形态和表型。细胞组织器官.192325-339（2010）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Cretoiu, D., Radu, B. M., Banciu, A., Banciu, D. D. & Cretoiu, S. M. Telocytes heterogeneity: From cellular morphology to functional evidence. Semin. Cell. Dev. Biol.64, 26–39 (2017).Article

Cretoiu，D.，Radu，B.M.，Banciu，A.，Banciu，D.D。和Cretoiu，S.M。端细胞异质性：从细胞形态学到功能证据。塞米。细胞。Dev.Biol.64,26-39（2017）。文章

PubMed

Google Scholar

谷歌学者

Marini, M. et al. Reappraising the microscopic anatomy of human testis: Identification of telocyte networks in the peritubular and intertubular stromal space. Sci. Rep.8, 14780 (2018).Article

Marini，M.等人。重新评估人类睾丸的显微解剖：鉴定管周和管间基质空间中的端细胞网络。科学。Rep.814780（2018）。文章

ADS

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Awad, M. & Ghanem, M. E. Localization of telocytes in rabbits testis: Histological and immunohistochemical approach. Microsc. Res. Tech.81, 1268–1274 (2018).Article

Awad，M。＆Ghanem，M.E。兔睾丸中端粒细胞的定位：组织学和免疫组织化学方法。微型计算机。Res.Tech.811268–1274（2018）。文章

PubMed

Google Scholar

谷歌学者

Hussein, M. T. & Abdel-Maksoud, F. M. Structural investigation of epididymal microvasculature and its relation to telocytes and immune cells in camel. Microsc. Microanal.26, 1024–1034 (2020).Article

Hussein，M.T。＆Abdel Maksoud，F.M。骆驼附睾微血管的结构研究及其与终末细胞和免疫细胞的关系。微型计算机。Microanal.261024-1034（2020）。文章

ADS

CAS

中科院

PubMed

Google Scholar

谷歌学者

Hoch, R. V. & Soriano, P. Roles of PDGF in animal development. Development130, 4769–4784 (2003).Article

Hoch，R.V。和Soriano，P。PDGF在动物发育中的作用。发展1304769-4784（2003）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Qian, C. et al. Stage specific requirement of platelet-derived growth factor receptor-α in embryonic development. PLoS One12, e0184473 (2017).Article

钱，C。等。胚胎发育中血小板衍生生长因子受体-α的阶段特异性要求。PLoS One12，e0184473（2017）。文章

PubMed

PubMed Central

公共医学中心

Google Scholar

谷歌学者

Basciani, S., Mariani, S., Spera, G. & Gnessi, L. Role of platelet-derived growth factors in the testis. Endocr. Rev.31, 916–939 (2010).Article

Basciani，S.，Mariani，S.，Spera，G。＆Gnessi，L。血小板衍生生长因子在睾丸中的作用。内分泌。Rev.31916–939（2010）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Bokemeyer, C. et al. Expression of stem-cell factor and its receptor c-kit protein in normal testicular tissue and malignant germ-cell tumours. J. Cancer Res. Clin. Oncol.122, 301–306 (1996).Article

Bokemeyer，C。等人。干细胞因子及其受体C-kit蛋白在正常睾丸组织和恶性生殖细胞肿瘤中的表达。J、癌症研究临床。Oncol.122301-306（1996）。文章

CAS

中科院

PubMed

Google Scholar

谷歌学者

Nurmio, M. et al. Inhibition of tyrosine kinases PDGFR and C-Kit by imatinib mesylate interferes with postnatal testicular development in the rat. Int. J. Androl.30, 366–376 (2007). discussion 376.Article

Nurmio，M。等人。甲磺酸伊马替尼抑制酪氨酸激酶PDGFR和C-Kit干扰大鼠出生后睾丸的发育。Int.J.Androl.30366–376（2007）。讨论376第条

CAS

中科院

PubMed

Google Scholar

谷歌学者

Terriac, E. et al. Vimentin levels and serine 71 phosphorylation in the control of cell-matrix adhesions, migration speed, and shape of transformed human fibroblasts. Cells6 (2017).Aumüller, G., Steinbrück, M., Krause, W. & Wagner, H. J. Distribution of vimentin-type intermediate filaments in sertoli cells of the human testis, normal and pathologic.

Terriac，E。等人。波形蛋白水平和丝氨酸71磷酸化在控制细胞基质粘附，迁移速度和转化的人成纤维细胞形状中的作用。细胞6（2017）。Aumüller，G.，Steinbrück，M.，Krause，W。＆Wagner，H.J。波形蛋白型中间丝在人睾丸支持细胞中的分布，正常和病理。

Anat. Embryol. (Berl)178, 129–136 (1988).Article .

阿纳特。胚胎。（贝尔）178129–136（1988）。第[UNK]条。

PubMed

Google Scholar

谷歌学者

Download referencesAcknowledgementsThe authors thank the Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, for their technical assistance in TEM.FundingThis study supported by the Natural Science Foundation of Gansu Province, China (Grant No. 23JRRA1420), National Nature Science Foundation of China (Grant No.

下载参考文献致谢作者感谢中国农业科学院兰州兽医研究所在TEM方面的技术援助。资助本研究由中国甘肃省自然科学基金（批准号23JRRA1420），国家自然科学基金（批准号：。

31160488) and The fund of Animal Clinical Practice Project of Gansu Agricultural University (NO. GSAU-JSFW-2023-07; NO. GSAU-JSFW-2023-08).Author informationAuthors and AffiliationsCollege of Veterinary Medicine, Gansu Agricultural University, Lanzhou, 730070, ChinaLong Ma, Ligang Yuan, Yumei Qi, Jianlin Zeng, Jinhan Lv & Xiaolong QieKey Laboratory of Animal Reproductive Physiology and Reproductive Regulation of Gansu Province, Lanzhou, 730070, ChinaLigang YuanAuthorsLong MaView author publicationsYou can also search for this author in.

31160488）和甘肃农业大学动物临床实践项目基金（编号GSAU-JSFW-2023-07；编号GSAU-JSFW-2023-08）。作者信息作者和附属机构甘肃农业大学兽医学院，兰州，730070，马龙，袁立刚，齐玉梅，曾建林，吕金汉和邱小龙甘肃省动物生殖生理学和生殖调控实验室，兰州，730070，袁立刚作者长MaView作者出版物您也可以在中搜索作者。

PubMed Google ScholarLigang YuanView author publicationsYou can also search for this author in

PubMed Google ScholarYumei QiView author publicationsYou can also search for this author in

PubMed Google ScholarYumei QiView作者出版物您也可以在

PubMed Google ScholarJianlin ZengView author publicationsYou can also search for this author in

PubMed Google ScholarJianlin ZengView作者出版物您也可以在

PubMed Google ScholarJinhan LvView author publicationsYou can also search for this author in

PubMed Google ScholarJinhan LvView作者出版物您也可以在

PubMed Google ScholarXiaolong QieView author publicationsYou can also search for this author in

PubMed谷歌学者肖龙QieView作者出版物您也可以在

PubMed Google ScholarContributionsL.M. designed the study, wrote the paper and analyzed the data. L.Y. critically reviewed the manuscript and modified the format. Y.Q. and J.Z. arranged the images. J.L. and X.Q. collected samples through surgery. All authors have read and approved the final version of the manuscript.Corresponding authorCorrespondence to.

PubMed谷歌学术贡献l。M、设计研究，撰写论文并分析数据。五十、 Y.批判性地审查了手稿并修改了格式。Y、 Q.和J.Z.安排了这些图像。J、 L.和X.Q.通过手术收集样本。所有作者都阅读并批准了稿件的最终版本。对应作者对应。

Ligang Yuan.Ethics declarations

袁立刚。道德宣言

Competing interests

相互竞争的利益

The authors declare no competing interests.

作者声明没有利益冲突。

Institutional review board statement

机构审查委员会声明

The study is reported in accordance with ARRIVE guidelines. And all experimental animals were approved by the Animal Care and Use Committee of the Veterinary College of Gansu Agricultural University (Ratification number: GSAU-Eth-VMC-2022-017).

。所有实验动物均经甘肃农业大学兽医学院动物护理与使用委员会批准（批准号：GSAU-Eth-VMC-2022-017）。

Additional informationPublisher’s noteSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.Rights and permissions

Additional informationPublisher的noteSpringer Nature在已发布地图和机构隶属关系中的管辖权主张方面保持中立。权限和权限

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material.

开放获取本文是根据知识共享署名非商业性NoDerivatives 4.0国际许可证授权的，该许可证允许以任何媒介或格式进行任何非商业性使用，共享，分发和复制，只要您对原始作者和来源给予适当的信任，提供知识共享许可证的链接，并指出您是否修改了许可材料。

You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.

To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/..

要查看此许可证的副本，请访问http://creativecommons.org/licenses/by-nc-nd/4.0/..

Reprints and permissionsAbout this articleCite this articleMa, L., Yuan, L., Qi, Y. et al. Morphological characteristics and distribution identification of telocytes in Tibetan sheep testis and epididymis.

转载和许可本文引用本文Ma，L.，Yuan，L.，Qi，Y。等人。藏绵羊睾丸和附睾中端粒细胞的形态特征和分布鉴定。

Sci Rep 14, 22783 (2024). https://doi.org/10.1038/s41598-024-73432-6Download citationReceived: 24 May 2024Accepted: 17 September 2024Published: 01 October 2024DOI: https://doi.org/10.1038/s41598-024-73432-6Share this articleAnyone you share the following link with will be able to read this content:Get shareable linkSorry, a shareable link is not currently available for this article.Copy to clipboard.

科学报告1422783（2024）。https://doi.org/10.1038/s41598-024-73432-6Download引文接收日期：2024年5月24日接受日期：2024年9月17日发布日期：2024年10月1日OI：https://doi.org/10.1038/s41598-024-73432-6Share本文与您共享以下链接的任何人都可以阅读此内容：获取可共享链接对不起，本文目前没有可共享的链接。复制到剪贴板。

Provided by the Springer Nature SharedIt content-sharing initiative

由Springer Nature SharedIt内容共享计划提供

CommentsBy submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

评论通过提交评论，您同意遵守我们的条款和社区指南。如果您发现有虐待行为或不符合我们的条款或准则，请将其标记为不合适。

全球产业链接平台

重庆市渝北区金星科技大厦A区5楼512室

联系电话：023-67139735（重庆）

关于我们

产品服务