AbstractAdipose tissue is crucial for energy storage and release, ensuring energy homeostasis within the body. Disturbances in the physiology of adipose tissue have been associated with various health disorders, such as obesity and diabetes. The reproductive cycle represents a fundamental biological pattern in female physiology.

摘要脂肪组织对于能量的储存和释放至关重要，可以确保体内的能量稳态。脂肪组织生理学的紊乱与各种健康障碍有关，例如肥胖和糖尿病。生殖周期代表了女性生理学的基本生物学模式。

Although previous research has highlighted the substantial regulatory influence of ovarian hormones on adipose tissue, our understanding of the comprehensive changes in adipose tissue throughout the reproductive cycle remains limited. In this study, we examined the transcriptomic profile of female mouse-adipose tissue across their complete estrous cycles.

尽管先前的研究强调了卵巢激素对脂肪组织的实质性调节作用，但我们对整个生殖周期中脂肪组织全面变化的理解仍然有限。在这项研究中，我们检查了雌性小鼠脂肪组织在整个发情周期中的转录组学特征。

The findings provided detailed descriptions of the datasets generated, including information on data collection, processing, and quality control. The study also demonstrated the robustness of these data through various validation steps. These findings serve as crucial resources for investigating the role of estrous cycle rhythmicity in important adipose tissue processes in the future..

这些发现提供了所生成数据集的详细描述，包括有关数据收集，处理和质量控制的信息。该研究还通过各种验证步骤证明了这些数据的稳健性。这些发现为研究动情周期节律性在未来重要脂肪组织过程中的作用提供了重要资源。。

Background & SummaryAdipose tissue plays a pivotal role in human physiology, functioning as an energy reservoir and endocrine tissue1,2. Dysregulation of adipose tissue functions, including adipogenesis, lipolysis, adipocytokines secretion, lipid metabolism, and thermogenesis, can result in a spectrum of pathological conditions such as metabolic disorders, obesity, type 2 diabetes, cardiovascular diseases, lipodystrophy, precocious puberty, among others1,2,3,4,5,6,7,8,9,10.

。脂肪组织功能失调，包括脂肪生成，脂解，脂肪细胞因子分泌，脂质代谢和产热，可导致一系列病理状况，如代谢紊乱，肥胖，2型糖尿病，心血管疾病，脂肪营养不良，性早熟等1,2,3,4,5,6,7,8,9,10。

As the prevalence of these adipose tissue-related disorders continues to increase, there is a growing global interest in adipose tissue research2.The reproductive cycle is a fundamental biological rhythm in female mammals; it is referred to as the menstrual cycle in women and higher-order primates and as the estrous cycle in non-primates11,12,13,14.

随着这些脂肪组织相关疾病的患病率持续增加，全球对脂肪组织研究的兴趣日益浓厚2。生殖周期是雌性哺乳动物的基本生物节律；它被称为女性和高级灵长类动物的月经周期，以及非灵长类动物的发情周期11,12,13,14。

During the reproductive cycle, female hormones like estrogen and progesterone undergo regular fluctuations that are associated with numerous metabolic activities13,15,16,17,18,19. Adipose tissue is widely recognized as a hormonally regulated tissue, and the regulatory effects of numerous individual hormones, such as insulin, estrogen, progesterone, testosterone, and thyroid hormones, on adipose tissue across multiple facets have been studied extensively20,21,22,23,24,25,26,27,28.

在生殖周期中，雌激素和孕激素等女性激素会发生规律性的波动，这与许多代谢活动有关13,15,16,17,18,19。脂肪组织被广泛认为是激素调节的组织，并且已经广泛研究了许多个体激素（例如胰岛素，雌激素，孕酮，睾酮和甲状腺激素）在多个方面对脂肪组织的调节作用20,21,22,23,24,25,26,27,28。

However, our understanding of how adipose tissue responds within the intricate, multi-hormonal physiological dynamics of the entire reproductive cycle remains limited.Transcriptional information is essential for elucidating the phenotype and functionality of adipose tissue. A previous study revealed the transcriptional variances in adipose tissue between estrus and diestrus; however, information regarding the other two stages of the estrus cycle is still lacking29.

然而，我们对脂肪组织在整个生殖周期复杂的多激素生理动力学中如何反应的理解仍然有限。转录信息对于阐明脂肪组织的表型和功能至关重要。先前的一项研究揭示了发情和发情之间脂肪组织的转录差异；然而，关于发情周期其他两个阶段的信息仍然缺乏29。

Therefore, in .

因此，在。

Code availability

代码可用性

This study employed entirely open-source software, and no custom code was developed to analyze the presented data.

这项研究采用了完全开源的软件，没有开发自定义代码来分析所提供的数据。

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Genome Sequence Archive https://ngdc.cncb.ac.cn/gsa/browse/CRA014866 (2024).Gene Expression Omnibus https://identifiers.org/geo:GSE275383 (2024).Download referencesAcknowledgementsThis research was supported by the National Key Research Program of China (Grant No. 2018YFC1004803 and 2020YFC1512704), and the Science and Technology Commission of Shanghai Municipality (Grant No.

基因组序列档案https://ngdc.cncb.ac.cn/gsa/browse/CRA014866（2024年）。基因表达综合https://identifiers.org/geo:GSE275383（2024年）。下载参考文献致谢本研究得到了中国国家重点研究计划（批准号2018YFC1004803和2020YFC1512704）和上海市科学技术委员会（批准号：。

22MC1940300). We extend our appreciation to Chao Fang for providing valuable technical support.Author informationAuthor notesThese authors contributed equally: Hongjie Zheng, Yier BaiThese authors jointly supervised this work: Qing Pei, Min Yao.Authors and AffiliationsDepartment of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Shanghai, 200011, ChinaHongjie Zheng, Yier Bai, Shan Wu, Zhixuan Jiang & Min YaoDepartment of Burn, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Shanghai, 200025, ChinaQing PeiAuthorsHongjie ZhengView author publicationsYou can also search for this author in.

22MC1940300）。我们感谢赵芳提供了宝贵的技术支持。作者信息作者注意到这些作者做出了同样的贡献：郑宏杰，易尔·拜特这些作者共同监督了这项工作：清培，姚敏。作者和附属机构上海交通大学医学院上海市第九人民医院整形外科，上海市芝枣居路639号，200011，中国郑红杰，白一叶，吴山，蒋志轩，姚敏上海交通大学医学院瑞金医院烧伤科，上海市瑞金二路197号，200025，中国青培作者郑红杰观点作者出版物您也可以在中搜索这位作者。

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PubMed Google ScholarContributionsH.Z., Q.P. and M.Y. designed the study. Q.P. and M.Y. supervised the study. H.Z., Y.B., S.W. and Z.J. conducted the experiments. H.Z. and Y.B. performed the data analyses and wrote the original draft. H.Z., Q.P. and M.Y. revised the manuscript, with approval of the final version for publication provided by all authors.Corresponding authorsCorrespondence to.

PubMed谷歌学术贡献。Z、 ，Q.P.和M.Y.设计了这项研究。Q、 P.和M.Y.监督了这项研究。H、 Z.，Y.B.，S.W.和Z.J.进行了实验。H、 Z.和Y.B.进行了数据分析并撰写了原始草案。H、 Z.，Q.P.和M.Y.修订了手稿，并批准了所有作者提供的最终版本。通讯作者通讯。

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清培或敏瑶。道德宣言

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Reprints and permissionsAbout this articleCite this articleZheng, H., Bai, Y., Wu, S. et al. Transcriptome dataset of mouse adipose tissue across estrous cycles.

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