3D X射线显微镜是颅面成像中准确有效的病理诊断设备-动脉网

3D X-ray microscope acts as an accurate and effective equipment of pathological diagnosis in craniofacial imaging

Nature 等信源发布 2024-10-07 15:05

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AbstractCraniofacial structure and dental hard tissue used to be researched on by traditional imaging tools such as light microscope, electron microscope and micro-CT. Due to the limitations of imaging principle, resolution and 3D rendering reconstruction technique, traditional imaging tools are constrained for presenting fine structure and precise measurements.

传统的成像工具如光学显微镜、电子显微镜和显微CT对颅面结构和牙齿硬组织进行研究。由于成像原理、分辨率和三维渲染重建技术的局限性，传统的成像工具在显示精细结构和精确测量方面受到限制。

Here a brand-new imaging equipment–3D X-ray microscope is introduced to realize a more efficient scanning by demonstrating the comparison of the craniofacial structures and dental hard tissue of diabetes and normal DBA mouse. To explore a higher resolution, more efficient imaging measurement and 3D reconstruction method on craniofacial structure and dental hard tissue.

这里介绍了一种全新的成像设备–3D X射线显微镜，通过演示糖尿病和正常DBA小鼠的颅面结构和牙齿硬组织的比较，实现了更有效的扫描。探索一种更高分辨率、更有效的颅面结构和牙齿硬组织的成像测量和三维重建方法。

The study included 12 DBA mice which were divided into two groups (control group and diabetes group). The heads were separated and scanned by 3D X-ray microscope, after which regions of interest were selected, followed by measurement and 3D reconstruction based on microscope attached software Dragonfly pro©.

该研究包括12只DBA小鼠，分为两组（对照组和糖尿病组）。将头部分离并通过3D X射线显微镜扫描，然后选择感兴趣的区域，然后基于显微镜附带的软件Dragonfly pro©进行测量和3D重建。

Hemi-mandibles were collected for enamel mineral density assessment supported by QRM-MicroCT-HA phantom. Data was submitted to paired t-tests at a 95% confidence level. The automatic assessed enamel thickness of diabetes mice decreased on average, whereas the rest of manual measurements and automatic assessed density showed no statistical difference.

收集半下颌骨用于由QRM MicroCT HA体模支持的牙釉质矿物质密度评估。数据以95%的置信水平提交给配对t检验。糖尿病小鼠的自动评估牙釉质厚度平均下降，而其余的手动测量和自动评估密度没有统计学差异。

We constructed HA phantom assisted enamel density procedure in Dragonfly software. Craniofacial structure and dental hard tissue were well-presented both in 2D slide and 3D reconstruction viewport by 3D X-ray microscope which can be routinely used as craniofacial structure and dental hard tissue imaging tool..

我们在Dragonfly软件中构建了HA幻影辅助的牙釉质密度程序。3D X射线显微镜在2D幻灯片和3D重建视口中均能很好地显示颅面结构和牙齿硬组织，可常规用作颅面结构和牙齿硬组织成像工具。。

IntroductionThe craniofacial structure are three-dimensionally arranged through 14 facial and 8 cranial bones that provide essential support and projection for the overlying soft tissues and organs, forming the cranium, sensory organs, mandible, temporomandibular joint (TMJ), palate, and other structures1.

引言颅面结构通过14个面部和8个颅骨三维排列，为上覆的软组织和器官提供必要的支撑和投影，形成颅骨，感觉器官，下颌骨，颞下颌关节（TMJ），腭和其他结构1。

The complexity of craniofacial structure was firstly proposed as “morphologie” by Johann Wolfgang von Goethe in 18th century2. Reconstructing and measuring this three-dimensional(3D) complex are one of the greatest challenges, especially for diagnosis and bioengineering of the craniofacial defects both in clinic and lab3.

颅面结构的复杂性在18世纪由约翰·沃尔夫冈·冯·歌德首次提出为“形态学”。重建和测量这种三维（3D）复合体是最大的挑战之一，特别是对于临床和lab3中颅面缺陷的诊断和生物工程而言。

Craniofacial phenotypic study has correlated genetic and epigenetic factors to improve our understanding of growth and development in health and disease, in which 3D imaging has played a key role in advancing craniofacial phenomics by facilitating highly sensitive and specific characterizations of craniofacial and dental morphology4.Teeth are complicated structures composed of several different mineralized tissues, namely enamel, dentine, and cementum, which enveloped a soft tissue, the dental pulp.

颅面表型研究将遗传和表观遗传因素联系起来，以提高我们对健康和疾病生长发育的理解，其中3D成像通过促进颅面和牙齿形态的高度敏感和特异性表征，在推进颅面表型方面发挥了关键作用。牙齿是由几种不同矿化组织组成的复杂结构，即牙釉质，牙本质和牙骨质，它们包裹着软组织牙髓。

The dental root anchors to the surrounding alveolar bone via short, tendon-like fibers called the periodontal ligament, which insert both into root cementum and bone5. Mature enamel is the hardest, most mineralized tissue in the human body, comprising > 95% by weight crystals of substituted calcium hydroxyapatite (HA; Ca10[PO4]6[OH]2)6, which thickness is 2–2.5 mm in average7.

牙根通过称为牙周膜的短腱样纤维锚定在周围的牙槽骨上，牙周膜插入牙根牙骨质和骨骼5。成熟的牙釉质是人体内最坚硬，矿化程度最高的组织，由取代的羟基磷灰石钙（HA；Ca10[PO4]6[OH]2）6的重量结晶组成，平均厚度为2-2.5毫米7。

The phenotype of enamel, like thickness and density can be affected by nature versus nurture factors. Enamel lacks any capacity for cellular repair and once formed, must function over a lifetime8. Thus, dental hard tissue measurements are vital for the observation of etiology and pa.

牙釉质的表型，如厚度和密度，可能受到先天因素和后天因素的影响。牙釉质缺乏任何细胞修复能力，一旦形成，必须在一生中发挥作用8。因此，牙齿硬组织测量对于观察病因和pa至关重要。

Data availability

数据可用性

Data is provided within the manuscript and the datasets used during the current study available from the first author on reasonable request.

数据在手稿中提供，当前研究中使用的数据集可根据第一作者的合理要求获得。

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Download referencesAcknowledgementsThanks to Dr. Zhaoyun Zhang, from Department of Endocrinology, Huashan Hospital Affiliated Fudan University, and Dr. Shengmei Qin from Department of Cardiology, Zhongshan Hospital Affiliated Fudan University, for providing rodents model. We are grateful to those who volunteered to participate in this study.

下载参考文献致谢感谢复旦大学附属华山医院内分泌科的张兆云博士和复旦大学附属中山医院心内科的秦盛美博士提供啮齿动物模型。我们感谢那些自愿参加这项研究的人。

The research, authorship, and/or publication of this article was supported by the grant of Open Fund Project of Shanghai Jiaotong University and Pinghu Intelligent Photoelectric Research Institute (2022SPIOE105), Science and Technology Commission of Shanghai (20ZR1431300), and Cross-disciplinary Research Fund of Shanghai Ninth People’s Hospital, Shanghai JiaoTong university School of Medicine (JYJC202311).Author informationAuthor notesThese authors contributed equally: Qianhui Ren and Zhao Yang.Authors and AffiliationsDepartment of Orthodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, 200011, People’s Republic of ChinaQianhui Ren, Jing Pan, Yangyang Li, Ying Guo, Mengning Bi, Yucong Zhou, Huiquan Yang & Fang JiSouth China Center of Craniofacial Stem Cell Research Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-Sen University, Guangzhou, 510080, People’s Republic of ChinaQianhui RenShanghai Jing’an Dental Clinic, Shanghai, 200040, People’s Republic of ChinaZhao YangSJTU-Pinghu Institute of Intelligent Optoelectronics, Pinghu, 314200, ChinaYilei Lu & Linjie ZhouState Key Laboratory of Advanced Optical Communication Systems and Networks, Shanghai Key La.

本文的研究，作者和/或发表得到了上海交通大学和平湖智能光电研究所开放基金项目（2022SPIOE105），上海市科学技术委员会（20ZR1431300）和上海市第九人民医院跨学科研究基金的资助。上海交通大学医学院（JYJC202311）。作者信息作者注意到这些作者做出了同样的贡献：任千辉和赵阳。作者和单位上海交通大学医学院上海市第九人民医院口腔正畸科，上海交通大学口腔医学院，国家口腔医学中心，国家口腔疾病临床研究中心，上海市口腔医学重点实验室，上海，200011，中华人民共和国任千辉，潘晶，李阳阳，郭莹，毕蒙宁，周玉聪，杨惠泉，方济华南口腔颅面干细胞研究中心，中山大学广东省口腔医学重点实验室，广州，510080，中华人民共和国上海市任千辉中华人民共和国上海市安牙科诊所，200040赵杨SJTU平湖智能光电子研究所，平湖，314200，中国易磊路和周林杰先进光通信系统与网络国家重点实验室，上海重点实验室。

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PubMed Google ScholarContributionsQianhui Ren and Zhao Yang wrote the main manuscript text; Yilei Lu prepared figures. All authors reviewed the manuscript.Corresponding authorsCorrespondence to

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Reprints and permissionsAbout this articleCite this articleRen, Q., Yang, Z., Lu, Y. et al. 3D X-ray microscope acts as an accurate and effective equipment of pathological diagnosis in craniofacial imaging.

转载和许可本文引用本文Ren，Q.，Yang，Z.，Lu，Y。等人。3D X射线显微镜是颅面成像中准确有效的病理诊断设备。

Sci Rep 14, 23275 (2024). https://doi.org/10.1038/s41598-024-74139-4Download citationReceived: 19 May 2024Accepted: 24 September 2024Published: 07 October 2024DOI: https://doi.org/10.1038/s41598-024-74139-4Share this articleAnyone you share the following link with will be able to read this content:Get shareable linkSorry, a shareable link is not currently available for this article.Copy to clipboard.

科学报告1423275（2024）。https://doi.org/10.1038/s41598-024-74139-4Download引文接收日期：2024年5月19日接受日期：2024年9月24日发布日期：2024年10月7日OI：https://doi.org/10.1038/s41598-024-74139-4Share。复制到剪贴板。

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Keywords3D X-ray microscope3D rendering reconstructionDental hard tissueCraniofacial structureEnamel measurementEnamel density

关键词3D X射线显微镜3D渲染重建牙齿硬组织颅面结构牙釉质测量纳米密度

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