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register hereObjective: Subarachnoid hemorrhage (SAH) and tumorigenesis share numerous biological complexities; nevertheless, the specific gene expression profiles and underlying mechanisms remain poorly understood. This study aims to identify differentially expressed genes (DEGs) that could serve as biomarkers for diagnosis and prognosis..

目的：蛛网膜下腔出血（SAH）和肿瘤发生具有许多生物学复杂性；。本研究旨在鉴定可作为诊断和预后生物标志物的差异表达基因（DEG）。。

Methods: Gene expression datasets (GSE122063, GSE13353, GSE161870) were analyzed using machine learning algorithms and logistic regression to identify DEGs associated with both SAH and tumorigenesis. Lasso regression and receiver operating characteristic (ROC) curve analysis were employed to evaluate the classification accuracy of these genes.

方法：使用机器学习算法和逻辑回归分析基因表达数据集（GSE122063，GSE13353，GSE161870），以鉴定与SAH和肿瘤发生相关的DEG。采用套索回归和受试者工作特征（ROC）曲线分析来评估这些基因的分类准确性。

Validation of critical DEGs was performed through pan-cancer analysis and experimental studies, focusing on the role of DOK3 in modulating inflammation and oxidative stress in U251MG glioblastoma and BV2 microglia cells..

通过泛癌分析和实验研究对关键DEG进行了验证，重点研究了DOK3在调节U251MG胶质母细胞瘤和BV2小胶质细胞炎症和氧化应激中的作用。。

Results: Fifteen common DEGs were identified, with DOK3 and PAPOLA highlighted as crucial genes implicated in SAH and neurodegenerative processes. Experimental validation demonstrated that DOK3 overexpression significantly reduced pro-inflammatory cytokine levels and oxidative stress markers while enhancing antioxidant enzyme activity.

结果：鉴定出15种常见的DEG，其中DOK3和丘疹突出显示为与SAH和神经退行性过程有关的关键基因。实验验证表明，DOK3过表达显着降低促炎细胞因子水平和氧化应激标志物，同时增强抗氧化酶活性。

Additionally, DOK3 influenced tumorigenic processes such as apoptosis, cell cycle regulation, and proliferation, effectively mitigating LPS-induced cytotoxicity and inflammation in BV2 microglial cells..

此外，DOK3影响致瘤过程，如细胞凋亡，细胞周期调节和增殖，有效减轻LPS诱导的BV2小胶质细胞的细胞毒性和炎症。。

Conclusions: DOK3 and PAPOLA play critical roles in both SAH and related neurodegeneration, presenting themselves as potential prognostic biomarkers and therapeutic targets. Notably, DOK3 exhibits potential as an antitumor agent with anti-inflammatory and antioxidative properties, offering therapeutic benefits for both cancer and neuroinflammatory conditions..

结论：DOK3和丘疹在SAH和相关神经退行性疾病中起着关键作用，成为潜在的预后生物标志物和治疗靶点。值得注意的是，DOK3具有作为具有抗炎和抗氧化特性的抗肿瘤剂的潜力，为癌症和神经炎症提供治疗益处。。