具有抗菌和再矿化双重功能的新型纳米材料在漱口水中的应用-动脉网

Application of novel nanomaterials with dual functions of antimicrobial and remineralization in mouthwashes

Nature 等信源发布 2024-11-23 18:54

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AbstractThe study aims to improve the antimicrobial and remineralization-promoting properties of mouthwash by synthesizing novel Ag/ZnO/Oyster Shells nanocomposites and evaluating their anti-caries properties and biosafety in vitro and in vivo, so as to reduce the incidence of caries. The antimicrobial properties of the synthesized Ag/ZnO/Oyster Shell nanocomposites were examined by bacterial inhibition zone, minimum inhibitory concentration, minimum bactericidal concentration, fluorescence staining and scanning electron microscopy.

摘要本研究旨在通过合成新型Ag/ZnO/牡蛎壳纳米复合材料，并在体内外评估其防龋性能和生物安全性，以提高漱口水的抗菌和促进再矿化性能，从而降低龋齿的发生率。通过细菌抑制区，最小抑制浓度，最小杀菌浓度，荧光染色和扫描电子显微镜检查合成的Ag/ZnO/牡蛎壳纳米复合材料的抗菌性能。

The potential of the materials to promote remineralization of demineralized enamel was detected by scanning electron microscopy, surface microhardness and depth of hard tissue defects, and laser confocal electron microscopy analysis. The synthesized materials were then incorporated into mouthwash, and their effects on antimicrobial properties, remineralization-promoting properties were evaluated.

通过扫描电子显微镜，表面显微硬度和硬组织缺陷深度以及激光共聚焦电子显微镜分析检测材料促进脱矿牙釉质再矿化的潜力。然后将合成的材料掺入漱口水中，并评估它们对抗菌性能，促进再矿化性能的影响。

Furthermore, an oral mucosal contact model was established to assess local irritation and systemic effects. The results showed that the novel Ag/ZnO/Oyster Shell nanocomposites possessed strong antimicrobial activity, remineralization-promoting ability and good biosafety, and the mouthwash containing Ag/ZnO/Oyster Shell possessed strong antimicrobial performance and remineralization-promoting ability, and showed no obvious abnormalities in local mucosal tissues, blood indices, and histopathology of the liver and kidneys in the oral exposure model of the SD rats.

此外，建立了口腔粘膜接触模型以评估局部刺激和全身效应。结果表明，新型Ag/ZnO/牡蛎壳纳米复合材料具有很强的抗菌活性，促进再矿化能力和良好的生物安全性，含有Ag/ZnO/牡蛎壳的漱口水具有很强的抗菌性能和促进再矿化能力，在SD大鼠口服暴露模型中，局部粘膜组织，血液指标以及肝脏和肾脏的组织病理学没有明显异常。

These findings indicate that Ag/ZnO/Oyster shell incorporated into mouthwash has strong antimicrobial activity, good remineralization-promoting properties and good biosafety in vivo. It is therefore expected to be used in clinical applications..

这些发现表明，掺入漱口水中的Ag/ZnO/牡蛎壳具有很强的抗菌活性，良好的再矿化促进特性和良好的体内生物安全性。因此，预计它将用于临床应用。。

IntroductionAccording to the recently published Global Burden Disease (GBD), dental caries is the most common disease worldwide, affecting nearly 2.3 billion people1,2. The Fourth National Oral Health Epidemiology Survey3published in August 2018 showed that the prevalence of caries among Chinese residents was on the rise, with 71.9% in the 5-year-old group, 38.5% in the 12-year-old group, 89.0% in the 35–44-year-old group, and 98.0% in the 65–74-year-old group.

引言根据最近发表的全球负担疾病（GBD），龋齿是全球最常见的疾病，影响近23亿人1,2。2018年8月发布的第四次全国口腔健康流行病学调查3显示，中国居民的龋齿患病率呈上升趋势，其中5岁组为71.9%，12岁组为38.5%，35-44岁组为89.0%，65-74岁组为98.0%。

The caries process is dynamic, with alternating demineralization and remineralization of tooth structure, and caries can be reversed or arrested even if the loss of minerals in the lesion is sufficient to manifest clinically as a white spot on the tooth surface4,5. Therefore, the key to caries control lies in prevention and routine maintenance, including preventing plaque formation, reducing enamel demineralization, and promoting remineralization of lesions6.Zinc oxide nanoparticles are potent antimicrobial agents because of their ability to accumulate on bacterial cell membranes by electrostatic attraction.

龋齿过程是动态的，牙齿结构交替脱矿和再矿化，即使病变中矿物质的损失足以在临床上表现为牙齿表面的白色斑点，龋齿也可以逆转或阻止4,5。因此，龋齿控制的关键在于预防和日常维护，包括防止斑块形成，减少牙釉质脱矿，促进病变的再矿化6。氧化锌纳米粒子是有效的抗菌剂，因为它们能够通过静电吸引在细菌细胞膜上积累。

In addition, it has osteogenic, anticancer and remineralization-promoting properties7,8,9. Mouthwashes containing zinc oxide nanoparticles have been shown to have high in vitro activity against cariogenic bacteria10and positively impact dentin by impeding its demineralization11. Meanwhile, silver nanoparticles have attracted broad attention owing to their low cost, nontoxicity, and high antimicrobial activity12,13,14,15.

此外，它具有成骨，抗癌和再矿化促进特性7,8,9。含有氧化锌纳米颗粒的漱口水已被证明对致龋细菌具有很高的体外活性10，并通过阻止其脱矿作用对牙本质产生积极影响11。同时，银纳米粒子由于其低成本，无毒性和高抗菌活性而受到广泛关注12,13,14,15。

According to previous studies, silver nanoparticles were found to not only improve the properties of zinc oxide nanoparticles, but also reduce the amount of ingredients16,17,18,19. In addition, it has also been shown that the combination of antimicrobial materials with calcium compounds (e.g..

根据以前的研究，发现银纳米粒子不仅可以改善氧化锌纳米粒子的性能，还可以减少元素的含量16,17,18,19。此外，还表明抗菌材料与钙化合物的组合（例如）。。

Data availability

数据可用性

Data is provided within the manuscript or supplementary information files.

数据在手稿或补充信息文件中提供。

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Download referencesAcknowledgementsNot applicable.FundingThe research project was supported by Natural Sciences Foundation of Fujian Province (grant number 2021J01801 and 2022J01767);Finance Department of Fujian Province (grant number 2021CZ01); the Open Project of Fujian Provincial Engineering Research Center of Oral Biomaterial (grant number 2021KQ01).Author informationAuthors and AffiliationsDepartment of Rende Road, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, Fujian Province, People’s Republic of ChinaLin ChangDepartment of Preventive Dentistry, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, Fujian Province, People’s Republic of ChinaRuihuan Gan, Xiaoyu Huang, Youguang Lu & Yan FengFujian Key Laboratory of Oral Diseases, School of Stomatology, Fujian Medical University, Fuzhou, Fujian Province, People’s Republic of ChinaLin Chang, Ruihuan Gan, Xiaoyu Huang, Dali Zheng, Chen Su, Youguang Lu & Yan FengAuthorsLin ChangView author publicationsYou can also search for this author in.

下载参考确认不适用。资助该研究项目由福建省自然科学基金（批准号2021J01801和2022J01767）资助；福建省财政厅（批准号2021CZ01）；。作者信息作者和所属单位福建医科大学口腔学院和医院仁德路教研室，福建省福州市福建医科大学口腔学院和医院预防牙科系，福建省福州市福建医科大学口腔学院和医院，福建省福州市，中华人民共和国福建医科大学口腔学院口腔疾病重点实验室，福建省福州市在。

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PubMed Google ScholarContributionsChang conducted the study and wrote the main manuscript; Gan and Huang reviewed and revised the manuscript; Su collected and processed the data; Zheng led the planning of the research activities; Feng and Lu managed and coordinated the execution of the research activities.

PubMed谷歌学术贡献Schang进行了这项研究并撰写了主要手稿；甘和黄审查并修改了手稿；苏收集并处理了数据；郑领导了研究活动的规划；冯和陆管理并协调了研究活动的执行。

All authors reviewed the manuscript.Corresponding authorsCorrespondence to.

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Reprints and permissionsAbout this articleCite this articleChang, L., Gan, R., Huang, X. et al. Application of novel nanomaterials with dual functions of antimicrobial and remineralization in mouthwashes.

转载和许可本文引用本文Chang，L.，Gan，R.，Huang，X。等人在漱口水中应用具有抗菌和再矿化双重功能的新型纳米材料。

Sci Rep 14, 29027 (2024). https://doi.org/10.1038/s41598-024-80703-9Download citationReceived: 03 July 2024Accepted: 21 November 2024Published: 23 November 2024DOI: https://doi.org/10.1038/s41598-024-80703-9Share this articleAnyone you share the following link with will be able to read this content:Get shareable linkSorry, a shareable link is not currently available for this article.Copy to clipboard.

Sci Rep 1429027（2024）。https://doi.org/10.1038/s41598-024-80703-9Download引文接收日期：2024年7月3日接受日期：2024年11月21日发布日期：2024年11月23日OI：https://doi.org/10.1038/s41598-024-80703-9Share本文与您共享以下链接的任何人都可以阅读此内容：获取可共享链接对不起，本文目前没有可共享的链接。。

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KeywordsNanocompositesMouthwashAntibacterialRemineralization propertiesBiosafety

关键词SnaNoCompositesMouthWash抗菌再矿化特性生物安全

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