/PRNewswire/ -- Cellecta, Inc. today announced the launch of the DriverMap™ EXP Human Genome-Wide 19K

/PRNewswire/ -- Cellecta, Inc. 今天宣布推出 DriverMap™ EXP 人类全基因组19K

Dried Blood Microsample Profiling kit, a targeted RNA expression profiling assay which provides a molecular snapshot of all 19,000 human protein-coding genes from as little as 30 microliters of dried whole blood.

干燥血液微样本分析试剂盒，一种靶向RNA表达谱分析方法，可以从低至30微升的干燥全血中提供所有19,000个人类蛋白质编码基因的分子快照。

microsamples: Blood collection and stabilization is carried out using Neoteryx VAMS microsamplers pre-treated with GenTegraRNA-NEO solution. Cellecta's DriverMap™ EXP Human Genome-Wide 19K Dried Blood Microsample Profiling assay is run, followed by NGS to obtain gene expression levels for 19,000 protein-coding genes..

微样本：使用预处理过GenTegraRNA-NEO溶液的Neoteryx VAMS微采样器进行血液采集和稳定化。随后运行Cellecta的DriverMap™ EXP人类全基因组19K干血微样本分析测定，接着通过NGS获取19,000个蛋白质编码基因的表达水平。

The assay combines multiplex RT-PCR and next-generation sequencing (NGS) enabling researchers to generate comprehensive, easy-to-analyze data from remotely collected droplets of blood captured using Trajan Scientific and Medical's Neoteryx

该检测结合了多重RT-PCR和下一代测序（NGS），使研究人员能够从使用Trajan Scientific and Medical的Neoteryx采集的远程收集的血液滴中生成全面且易于分析的数据。

microsample collection kits containing Mitra

含有Mitra的微样本采集工具包

devices based on VAMS

基于VAMS的设备

technology. Until now, it has not been possible to effectively assay gene transcription from remotely collected blood microsamples because of the instability of RNA in conventionally collected blood samples, and the volume required for transcriptome profiling approaches such as RNA-Seq and microarrays.

技术。直到现在，由于常规收集的血液样本中RNA的不稳定性，以及进行转录组分析（如RNA-Seq和微阵列）所需的样本量，无法有效地从远程收集的血液微样本中检测基因转录。

The combination of the Mitra microsampling device with the DriverMap EXP Profiling assay's sensitivity addresses these challenges, allowing for convenient collection, stabilization, and comprehensive profiling from just 30ml of dried, whole blood..

Mitra微采样装置与DriverMap EXP分析方法的敏感性相结合，解决了这些挑战，只需30毫升干燥的全血，便可实现方便的收集、稳定化和全面分析。

'We have shown that using dried blood microsamples as starting material for the DriverMap EXP profiling assay has been as successful as using standard volumes of whole blood in obtaining differential expression-level data of thousands of genes,' said

“我们已经证明，使用干燥血液微样本作为DriverMap EXP分析的起始材料，与使用标准体积的全血一样成功，能够获取数千个基因的差异表达水平数据，”

Alex Chenchik

亚历克斯·陈奇克

, Ph.D., president and chief scientific officer of Cellecta. 'Now, with this convenient, low-volume blood collection option, we foresee the assay's utility extending to many new research applications, such as multi-sample, longitudinal studies for discovery and validation of transcriptome biomarkers for prognostic and diagnostic use.'.

博士，Cellecta公司的总裁兼首席科学官。“现在，有了这种便捷的低量血液采集选项，我们预计该检测的用途将扩展到许多新的研究应用中，例如用于发现和验证转录组生物标志物的多样本纵向研究，以实现预后和诊断用途。”

The DriverMap™ EXP Dried Blood Microsample Profiling kit is highly sensitive and reproducible. This expression profiling assay involves a simple, single-day workflow that includes:

DriverMap™ EXP 干血微量样本分析试剂盒具有高灵敏度和可重复性。该表达谱分析实验包括一个简单、单日的工作流程，其中包括：

Blood collection from a fingertip after lancet puncture by absorption onto VAMS sampling tips (contained within the Mitra device) pre-coated for stabilization of RNA with the GenTegraRNA-NEO™ solution, which confers stability at room temperature for up to 7 days. The Mitra devices and the GenTegraRNA-NEO solution are sold separately by Neoteryx (.

使用含VAMS采样头的Mitra设备（预涂有GenTegraRNA-NEO™溶液以稳定RNA）从指尖采集血液样本，该溶液可在室温下提供长达7天的稳定性。Mitra设备和GenTegraRNA-NEO溶液由Neoteryx公司分别销售。

RNA extraction from the VAMS tips using a specialized buffer solution included in the DriverMap EXP Dried Blood kit, followed by the expression profiling assay—hybridization of mRNA, cDNA synthesis, primer extension, and subsequent PCR steps--for next-generation sequencing (NGS) library preparation, and sequencing on an Illumina instrument..

使用DriverMap EXP干血试剂盒中的专用缓冲液从VAMS尖端提取RNA，随后进行表达谱分析——mRNA杂交、cDNA合成、引物延伸及后续PCR步骤——用于下一代测序（NGS）文库制备，并在Illumina仪器上进行测序。

Data processing of FASTQ files and analysis using open-source Salmon software, or Cellecta Bioinformatics services to obtain the gene counts and study the differential gene expression levels of the samples.

使用开源的 Salmon 软件对 FASTQ 文件进行数据处理和分析，或利用 Cellecta 生物信息学服务获取基因计数并研究样品的差异基因表达水平。

The DriverMap™ EXP Dried Blood kit includes a complete set of gene-specific and PCR-NGS primers, positive control RNAs, and all other reagents required to profile blood microsamples and prepare them for digital expression profiling on an Illumina sequencer.

DriverMap™ EXP 干血试剂盒包含全套基因特异性和PCR-NGS引物、阳性对照RNA以及在Illumina测序仪上进行数字化表达谱分析所需的全部其他试剂。

The DriverMap™ EXP Human Genome-Wide

DriverMap™ EXP 人类全基因组

19K

19K

Dried Blood Microsample Profiling kit is available now in two configurations, for 24 or 96 samples. For more information, please visit Cellecta.com/drivermap

干血微样本分析试剂盒现已推出两种配置，适用于24或96个样本。欲了解更多信息，请访问Cellecta.com/drivermap。

About Cellecta:

关于Cellecta：

Cellecta, Inc. is a trusted provider of genomic products and services that advance research for drug target and biomarker discovery. Since 2006, we have collaborated with the world's leading pharma, biotech, government, and academic institutions, applying our expertise in viral vector production, functional screening, cell engineering and multiplex RT-qPCR to help researchers find the genes that matter for drug target and biomarker discovery and validation..

Cellecta, Inc. 是一家值得信赖的基因组产品和服务提供商，致力于推动药物靶点和生物标志物发现的研究。自2006年以来，我们与全球领先的制药、生物技术、政府和学术机构合作，运用我们在病毒载体生产、功能筛选、细胞工程和多重RT-qPCR方面的专业知识，帮助研究人员找到对药物靶点和生物标志物的发现及验证至关重要的基因。

Mitra® devices are CE-IVD (IVDR) devices intended as a specimen collector, and for the storage and transport of blood and other biological fluids for analytical and diagnostic analyses. They are available as registered IVD Devices in the European Union and

Mitra® 设备是CE-IVD（IVDR）设备，用作样本收集器，用于血液和其他生物液体的存储和运输，以进行分析和诊断分析。它们在欧盟作为注册的IVD设备提供，并且

, Mitra devices are supplied as a research use only (RUO) product to assist in method development, other research-related and non-diagnostic activities. End-users and laboratories must validate the use of the Mitra devices for the particular diagnostic testing intended.

Mitra设备作为仅供研究使用（RUO）的产品提供，用于协助方法开发、其他与研究相关的和非诊断活动。最终用户和实验室必须验证Mitra设备在特定诊断测试中的使用。